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GATA1-deficient dendritic cells display impaired CCL21-dependent migration toward lymph nodes due to reduced levels of polysialic acid

Maaike R Scheenstra, Iris M De Cuyper, Filipe Branco Madeira UGent, Pieter De Bleser UGent, Mirjam Kool, Marjolein Meinders, Mark Hoogenboezem, Erik Mu, Monika C Wolkers, Fiamma Salerno, et al. (2016) JOURNAL OF IMMUNOLOGY. 197(11). p.4312-4324
abstract
Dendritic cells (DCs) play a pivotal role in the regulation of the immune response. DC development and activation is finely orchestrated through transcriptional programs. GATA1 transcription factor is required for murine DC development, and data suggest that it might be involved in the fine-tuning of the life span and function of activated DCs. We generated DC-specific Gatal knockout mice (Gatal-KODc), which presented a 20% reduction of splenic DCs, partially explained by enhanced apoptosis. RNA sequencing analysis revealed a number of deregulated genes involved in cell survival, migration, and function. DC migration toward peripheral lymph nodes was impaired in Gatal-KODc mice. Migration assays performed in vitro showed that this defect was selective for CCL21, but not CCL19. Interestingly, we show that Gatal-KODc DCs have reduced polysialic acid levels on their surface, which is a known determinant for the proper migration of DCs toward CCL21.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SUBSETS IN-VIVO, TRANSCRIPTION FACTOR, STEADY-STATE, REGULATORY, FACTOR-4, GENE-EXPRESSION, MICE DEFICIENT, ADULT MICE, MOUSE, DIFFERENTIATION, CCL21
journal title
JOURNAL OF IMMUNOLOGY
J. Immunol.
volume
197
issue
11
pages
4312 - 4324
Web of Science type
Article
Web of Science id
000389635300018
JCR category
IMMUNOLOGY
JCR impact factor
4.856 (2016)
JCR rank
33/150 (2016)
JCR quartile
1 (2016)
ISSN
0022-1767
1550-6606
DOI
10.4049/jimmunol.1600103
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8507527
handle
http://hdl.handle.net/1854/LU-8507527
date created
2017-02-03 16:10:22
date last changed
2017-02-28 11:16:39
@article{8507527,
  abstract     = {Dendritic cells (DCs) play a pivotal role in the regulation of the immune response. DC development and activation is finely orchestrated through transcriptional programs. GATA1 transcription factor is required for murine DC development, and data suggest that it might be involved in the fine-tuning of the life span and function of activated DCs. We generated DC-specific Gatal knockout mice (Gatal-KODc), which presented a 20\% reduction of splenic DCs, partially explained by enhanced apoptosis. RNA sequencing analysis revealed a number of deregulated genes involved in cell survival, migration, and function. DC migration toward peripheral lymph nodes was impaired in Gatal-KODc mice. Migration assays performed in vitro showed that this defect was selective for CCL21, but not CCL19. Interestingly, we show that Gatal-KODc DCs have reduced polysialic acid levels on their surface, which is a known determinant for the proper migration of DCs toward CCL21.},
  author       = {Scheenstra, Maaike R and De Cuyper, Iris M and Branco Madeira, Filipe and De Bleser, Pieter and Kool, Mirjam and Meinders, Marjolein and Hoogenboezem, Mark and Mu, Erik and Wolkers, Monika C and Salerno, Fiamma and Nota, Benjamin and Saeys, Yvan and Klarenbeek, Sjoerd and van IJcken, Wilfred FJ and Hammad, Hamida and Philipsen, Sjaak and van den Berg, Timo K and Kuijpers, Taco W and Lambrecht, Bart and Gutierrez, Laura},
  issn         = {0022-1767},
  journal      = {JOURNAL OF IMMUNOLOGY},
  keyword      = {SUBSETS IN-VIVO,TRANSCRIPTION FACTOR,STEADY-STATE,REGULATORY,FACTOR-4,GENE-EXPRESSION,MICE DEFICIENT,ADULT MICE,MOUSE,DIFFERENTIATION,CCL21},
  language     = {eng},
  number       = {11},
  pages        = {4312--4324},
  title        = {GATA1-deficient dendritic cells display impaired CCL21-dependent migration toward lymph nodes due to reduced levels of polysialic acid},
  url          = {http://dx.doi.org/10.4049/jimmunol.1600103},
  volume       = {197},
  year         = {2016},
}

Chicago
Scheenstra, Maaike R, Iris M De Cuyper, Filipe Branco Madeira, Pieter De Bleser, Mirjam Kool, Marjolein Meinders, Mark Hoogenboezem, et al. 2016. “GATA1-deficient Dendritic Cells Display Impaired CCL21-dependent Migration Toward Lymph Nodes Due to Reduced Levels of Polysialic Acid.” Journal of Immunology 197 (11): 4312–4324.
APA
Scheenstra, M. R., De Cuyper, I. M., Branco Madeira, F., De Bleser, P., Kool, M., Meinders, M., Hoogenboezem, M., et al. (2016). GATA1-deficient dendritic cells display impaired CCL21-dependent migration toward lymph nodes due to reduced levels of polysialic acid. JOURNAL OF IMMUNOLOGY, 197(11), 4312–4324.
Vancouver
1.
Scheenstra MR, De Cuyper IM, Branco Madeira F, De Bleser P, Kool M, Meinders M, et al. GATA1-deficient dendritic cells display impaired CCL21-dependent migration toward lymph nodes due to reduced levels of polysialic acid. JOURNAL OF IMMUNOLOGY. 2016;197(11):4312–24.
MLA
Scheenstra, Maaike R, Iris M De Cuyper, Filipe Branco Madeira, et al. “GATA1-deficient Dendritic Cells Display Impaired CCL21-dependent Migration Toward Lymph Nodes Due to Reduced Levels of Polysialic Acid.” JOURNAL OF IMMUNOLOGY 197.11 (2016): 4312–4324. Print.