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RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)

Author
Organization
Project
RENEB (Realizing the European Network in Biodosimetry)
Abstract
Purpose: Two quality controlled inter-laboratory exercises were organized within the EU project ‘Realizing the European Network of Biodosimetry (RENEB)’ to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network. Materials and methods: The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95% confidence intervals were submitted by the participants. Results: The shipment of blood samples to the partners in the European Community (EU) were performed successfully. Outside the EU unacceptable delays occurred. The results of the dose estimation demonstrate a very successful classification of the blood samples in medically relevant groups. In comparison to the 1st exercise the 2nd intercomparison showed an improvement in the accuracy of dose estimations especially for the high dose point. Conclusions: In case of a large-scale radiological incident, the pooling of ressources by networks can enhance the rapid classification of individuals in medically relevant treatment groups based on the DCA. The performance of the RENEB network as a whole has clearly benefited from harmonization processes and specific training activities for the network partners.
Keywords
Biological dosimetry, dicentric chromosomes, intercomparison, network, RENEB, RADIATION BIODOSIMETRY, BIOLOGICAL DOSIMETRY, EUROPEAN NETWORK, TRIAGE, CASUALTIES, MODE

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Citation

Please use this url to cite or link to this publication:

Chicago
Oestreicher, Ursula, Daniel Samaga, Elizabeth Ainsbury, Ana Catarina Antunes, Ans Baeyens, Leonardo Barrios, Christina Beinke, et al. 2017. “RENEB Intercomparisons Applying the Conventional Dicentric Chromosome Assay (DCA).” International Journal of Radiation Biology 93 (1): 20–29.
APA
Oestreicher, U., Samaga, D., Ainsbury, E., Antunes, A. C., Baeyens, A., Barrios, L., Beinke, C., et al. (2017). RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 93(1), 20–29.
Vancouver
1.
Oestreicher U, Samaga D, Ainsbury E, Antunes AC, Baeyens A, Barrios L, et al. RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2017;93(1):20–9.
MLA
Oestreicher, Ursula, Daniel Samaga, Elizabeth Ainsbury, et al. “RENEB Intercomparisons Applying the Conventional Dicentric Chromosome Assay (DCA).” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 93.1 (2017): 20–29. Print.
@article{8507326,
  abstract     = {Purpose: Two quality controlled inter-laboratory exercises were organized within the EU project {\textquoteleft}Realizing the European Network of Biodosimetry (RENEB){\textquoteright} to further optimize the dicentric chromosome assay (DCA) and to identify needs for training and harmonization activities within the RENEB network.
Materials and methods: The general study design included blood shipment, sample processing, analysis of chromosome aberrations and radiation dose assessment. After manual scoring of dicentric chromosomes in different cell numbers dose estimations and corresponding 95\% confidence intervals were submitted by the participants.
Results: The shipment of blood samples to the partners in the European Community (EU) were performed successfully. Outside the EU unacceptable delays occurred. The results of the dose estimation demonstrate a very successful classification of the blood samples in medically relevant groups. In comparison to the 1st exercise the 2nd intercomparison showed an improvement in the accuracy of dose estimations especially for the high dose point.
Conclusions: In case of a large-scale radiological incident, the pooling of ressources by networks can enhance the rapid classification of individuals in medically relevant treatment groups based on the DCA. The performance of the RENEB network as a whole has clearly benefited from harmonization processes and specific training activities for the network partners.},
  author       = {Oestreicher, Ursula and Samaga, Daniel and Ainsbury, Elizabeth and Antunes, Ana Catarina and Baeyens, Ans and Barrios, Leonardo and Beinke, Christina and Beukes, Philip and Blakely, William F. and Cucu, Alexandra and De Amicis, Andrea and Depuydt, Julie and De Sanctis, Stefania and Di Giorgio, Marina and Dobos, Katalin and Dominguez, Inmaculada and Duy, Pham Ngoc and Espinoza, Marco E. and Flegal, Farrah N. and Figel, Markus and Garcia, Omar and Monteiro Gil, Oct{\'a}via and Gregoire, Eric and Guerrero-Carbajal, C. and G{\"u}\c{c}l{\"u}, \.{I}nci and Hadjidekova, Valeria and Hande, Prakash and Kulka, Ulrike and Lemon, Jennifer and Lindholm, Carita and Lista, Florigio and Lumniczky, Katalin and Martinez-Lopez, Wilner and Maznyk, Nataliya and Meschini, Roberta and M{\textquoteright}kacher, Radia and Montoro, Alegria and Moquet, Jayne and Moreno, Mercedes and Noditi, Mihaela and Pajic, Jelena and Radl, Anal{\'i}a and Ricoul, Michelle and Romm, Horst and Roy, Laurence and Sabatier, Laure and Sebasti{\`a}, Natividad and Slabbert, Jacobus and Sommer, Sylwester and Stuck Oliveira, Monica and Subramanian, Uma and Suto, Yumiko and Que, Tran and Testa, Antonella and Terzoudi, Georgia and Vral, Anne and Wilkins, Ruth and Yanti, LusiYanti and Zafiropoulos, Demetre and Wojcik, Andrzej},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {20--29},
  title        = {RENEB intercomparisons applying the conventional Dicentric Chromosome Assay (DCA)},
  url          = {http://dx.doi.org/10.1080/09553002.2016.1233370},
  volume       = {93},
  year         = {2017},
}

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