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Microfluidic flow chambers using reconstituted blood to model hemostasis and platelet transfusion in vitro

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Abstract
Blood platelets prepared for transfusion gradually lose hemostatic function during storage. Platelet function can be investigated using a variety of (indirect) in vitro experiments, but none of these is as comprehensive as microfluidic flow chambers. In this protocol, the reconstitution of thrombocytopenic fresh blood with stored blood bank platelets is used to simulate platelet transfusion. Next, the reconstituted sample is perfused in microfluidic flow chambers which mimic hemostasis on exposed subendothelial matrix proteins. Effects of blood donation, transport, component separation, storage and pathogen inactivation can be measured in paired experimental designs. This allows reliable comparison of the impact every manipulation in blood component preparation has on hemostasis. Our results demonstrate the impact of temperature cycling, shear rates, platelet concentration and storage duration on platelet function. In conclusion, this protocol analyzes the function of blood bank platelets and this ultimately aids in optimization of the processing chain including phlebotomy, transport, component preparation, storage and transfusion.
Keywords
Bioengineering, Issue 109, Platelets, microfluidic flow chamber, shear stress, transfusion, blood reconstitution, standardization, MEASURE THROMBUS FORMATION, VON-WILLEBRAND-FACTOR, GLOBAL ASSESSMENT, STANDARDIZATION, ASSAYS, AGGREGATION, ADHESION

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Citation

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Chicago
Van Aelst, Britt, Hendrik Feys, Rosalie Devloo, Philippe Vandekerckhove, and Veerle Compernolle. 2016. “Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion in Vitro.” Jove-journal of Visualized Experiments (109).
APA
Van Aelst, Britt, Feys, H., Devloo, R., Vandekerckhove, P., & Compernolle, V. (2016). Microfluidic flow chambers using reconstituted blood to model hemostasis and platelet transfusion in vitro. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (109).
Vancouver
1.
Van Aelst B, Feys H, Devloo R, Vandekerckhove P, Compernolle V. Microfluidic flow chambers using reconstituted blood to model hemostasis and platelet transfusion in vitro. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS. 2016;(109).
MLA
Van Aelst, Britt, Hendrik Feys, Rosalie Devloo, et al. “Microfluidic Flow Chambers Using Reconstituted Blood to Model Hemostasis and Platelet Transfusion in Vitro.” JOVE-JOURNAL OF VISUALIZED EXPERIMENTS 109 (2016): n. pag. Print.
@article{8507092,
  abstract     = {Blood platelets prepared for transfusion gradually lose hemostatic function during storage. Platelet function can be investigated using a variety of (indirect) in vitro experiments, but none of these is as comprehensive as microfluidic flow chambers. In this protocol, the reconstitution of thrombocytopenic fresh blood with stored blood bank platelets is used to simulate platelet transfusion. Next, the reconstituted sample is perfused in microfluidic flow chambers which mimic hemostasis on exposed subendothelial matrix proteins. Effects of blood donation, transport, component separation, storage and pathogen inactivation can be measured in paired experimental designs. This allows reliable comparison of the impact every manipulation in blood component preparation has on hemostasis. Our results demonstrate the impact of temperature cycling, shear rates, platelet concentration and storage duration on platelet function. In conclusion, this protocol analyzes the function of blood bank platelets and this ultimately aids in optimization of the processing chain including phlebotomy, transport, component preparation, storage and transfusion.},
  articleno    = {e53823},
  author       = {Van Aelst, Britt and Feys, Hendrik and Devloo, Rosalie and Vandekerckhove, Philippe and Compernolle, Veerle},
  issn         = {1940-087X},
  journal      = {JOVE-JOURNAL OF VISUALIZED EXPERIMENTS},
  language     = {eng},
  number       = {109},
  pages        = {10},
  title        = {Microfluidic flow chambers using reconstituted blood to model hemostasis and platelet transfusion in vitro},
  url          = {http://dx.doi.org/10.3791/53823},
  year         = {2016},
}

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