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Quantification of in vivo colonic short chain fatty acid production from inulin

(2015) NUTRIENTS. 7(11). p.8916-8929
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Abstract
Short chain fatty acids ( SCFA), including acetate, propionate, and butyrate, are produced during bacterial fermentation of undigested carbohydrates in the human colon. In this study, we applied a stable-isotope dilution method to quantify the in vivo colonic production of SCFA in healthy humans after consumption of inulin. Twelve healthy subjects performed a test day during which a primed continuous intravenous infusion with [ 1- C-13] acetate, [ 1- C-13] propionate and [ 1- C-13] butyrate ( 12, 1.2 and 0.6 mu mol center dot kg - 1 center dot min - 1, respectively) was applied. They consumed 15 g of inulin with a standard breakfast. Breath and blood samples were collected at regular times during the day over a 12 h period. The endogenous rate of appearance of acetate, propionate, and butyrate was 13.3 +/- 4.8, 0.27 +/- 0.09, and 0.28 +/- 0.12 mu mol center dot kg - 1 center dot min (-1), respectively. Colonic inulin fermentation was estimated to be 137 +/- 75 mmol acetate, 11 +/- 9 mmol propionate, and 20 +/- 17 mmol butyrate over 12 h, assuming that 40%, 10%, and 5% of colonic derived acetate, propionate, and butyrate enter the systemic circulation. In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate. Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.
Keywords
short chain fatty acids, colonic fermentation, inulin, HUMAN LARGE-INTESTINE, DISTINCT FERMENTATION PATTERNS, BUTYRATE-PRODUCING BACTERIA, HUMAN FECAL MICROFLORA, VITRO FERMENTATION, GUT MICROBIOTA, WHEAT DEXTRIN, PROPIONATE, HUMANS, ACETATE

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Chicago
Boets, Eef, Lise Deroover, Els Houben, Karen Vermeulen, Sara Gomand, Jan Delcour, and Kristin Verbeke. 2015. “Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.” Nutrients 7 (11): 8916–8929.
APA
Boets, E., Deroover, L., Houben, E., Vermeulen, K., Gomand, S., Delcour, J., & Verbeke, K. (2015). Quantification of in vivo colonic short chain fatty acid production from inulin. NUTRIENTS, 7(11), 8916–8929.
Vancouver
1.
Boets E, Deroover L, Houben E, Vermeulen K, Gomand S, Delcour J, et al. Quantification of in vivo colonic short chain fatty acid production from inulin. NUTRIENTS. 2015;7(11):8916–29.
MLA
Boets, Eef, Lise Deroover, Els Houben, et al. “Quantification of in Vivo Colonic Short Chain Fatty Acid Production from Inulin.” NUTRIENTS 7.11 (2015): 8916–8929. Print.
@article{8506948,
  abstract     = {Short chain fatty acids ( SCFA), including acetate, propionate, and butyrate, are produced during bacterial fermentation of undigested carbohydrates in the human colon. In this study, we applied a stable-isotope dilution method to quantify the in vivo colonic production of SCFA in healthy humans after consumption of inulin. Twelve healthy subjects performed a test day during which a primed continuous intravenous infusion with [ 1- C-13] acetate, [ 1- C-13] propionate and [ 1- C-13] butyrate ( 12, 1.2 and 0.6 mu mol center dot kg - 1 center dot min - 1, respectively) was applied. They consumed 15 g of inulin with a standard breakfast. Breath and blood samples were collected at regular times during the day over a 12 h period. The endogenous rate of appearance of acetate, propionate, and butyrate was 13.3 +/- 4.8, 0.27 +/- 0.09, and 0.28 +/- 0.12 mu mol center dot kg - 1 center dot min (-1), respectively. Colonic inulin fermentation was estimated to be 137 +/- 75 mmol acetate, 11 +/- 9 mmol propionate, and 20 +/- 17 mmol butyrate over 12 h, assuming that 40\%, 10\%, and 5\% of colonic derived acetate, propionate, and butyrate enter the systemic circulation. In conclusion, inulin is mainly fermented into acetate and, to lesser extents, into butyrate and propionate. Stable isotope technology allows quantifying the production of the three main SCFA in vivo and proved to be a practical tool to investigate the extent and pattern of SCFA production.},
  author       = {Boets, Eef and Deroover, Lise and Houben, Els and Vermeulen, Karen and Gomand, Sara and Delcour, Jan and Verbeke, Kristin},
  issn         = {2072-6643},
  journal      = {NUTRIENTS},
  language     = {eng},
  number       = {11},
  pages        = {8916--8929},
  title        = {Quantification of in vivo colonic short chain fatty acid production from inulin},
  url          = {http://dx.doi.org/10.3390/nu7115440},
  volume       = {7},
  year         = {2015},
}

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