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Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy

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Abstract
The porcine pathogen Chlamydia suis is widespread in pig farming. Isolation of Chlamydia suis in cell culture is crucial for the generation and characterization of new isolates. However, isolation of Chlamydia suis strains from field samples is fastidious. Therefore, we exploited high-content microscopy to quantify the growth of Chlamydia suis strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation. We conclude that adaptation of the propagation procedure to the origin of the putative field isolate is highly recommended to improve the recovery rate.
Keywords
Chlamydia, cell culture, high-content microscopy, isolation, swine, GNOTOBIOTIC PIGS, REPRODUCTIVE FAILURE, IN-VITRO, INFECTION, SWINE, CONJUNCTIVITIS, PREVALENCE, PSITTACI, PIGLETS, STRAIN

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Chicago
De Puysseleyr, Leentje, Kristien De Puysseleyr, Daisy Vanrompay, and Winnok De Vos. 2017. “Quantifying the Growth of Chlamydia Suis in Cell Culture Using High-content Microscopy.” Microscopy Research and Technique 80 (4): 350–356.
APA
De Puysseleyr, L., De Puysseleyr, K., Vanrompay, D., & De Vos, W. (2017). Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy. MICROSCOPY RESEARCH AND TECHNIQUE, 80(4), 350–356.
Vancouver
1.
De Puysseleyr L, De Puysseleyr K, Vanrompay D, De Vos W. Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy. MICROSCOPY RESEARCH AND TECHNIQUE. 2017;80(4):350–6.
MLA
De Puysseleyr, Leentje, Kristien De Puysseleyr, Daisy Vanrompay, et al. “Quantifying the Growth of Chlamydia Suis in Cell Culture Using High-content Microscopy.” MICROSCOPY RESEARCH AND TECHNIQUE 80.4 (2017): 350–356. Print.
@article{8506862,
  abstract     = {The porcine pathogen Chlamydia suis is widespread in pig farming. Isolation of Chlamydia suis in cell culture is crucial for the generation and characterization of new isolates. However, isolation of Chlamydia suis strains from field samples is fastidious. Therefore, we exploited high-content microscopy to quantify the growth of Chlamydia suis strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation. We conclude that adaptation of the propagation procedure to the origin of the putative field isolate is highly recommended to improve the recovery rate.},
  author       = {De Puysseleyr, Leentje and De Puysseleyr, Kristien and Vanrompay, Daisy and De Vos, Winnok},
  issn         = {1059-910X},
  journal      = {MICROSCOPY RESEARCH AND TECHNIQUE},
  keyword      = {Chlamydia,cell culture,high-content microscopy,isolation,swine,GNOTOBIOTIC PIGS,REPRODUCTIVE FAILURE,IN-VITRO,INFECTION,SWINE,CONJUNCTIVITIS,PREVALENCE,PSITTACI,PIGLETS,STRAIN},
  language     = {eng},
  number       = {4},
  pages        = {350--356},
  title        = {Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy},
  url          = {http://dx.doi.org/10.1002/jemt.22799},
  volume       = {80},
  year         = {2017},
}

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