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Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy

Leentje De Puysseleyr, Kristien De Puysseleyr, Daisy Vanrompay UGent and Winnok De Vos UGent (2017) MICROSCOPY RESEARCH AND TECHNIQUE. 80(4). p.350-356
abstract
The porcine pathogen Chlamydia suis is widespread in pig farming. Isolation of Chlamydia suis in cell culture is crucial for the generation and characterization of new isolates. However, isolation of Chlamydia suis strains from field samples is fastidious. Therefore, we exploited high-content microscopy to quantify the growth of Chlamydia suis strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation. We conclude that adaptation of the propagation procedure to the origin of the putative field isolate is highly recommended to improve the recovery rate.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Chlamydia, cell culture, high-content microscopy, isolation, swine, GNOTOBIOTIC PIGS, REPRODUCTIVE FAILURE, IN-VITRO, INFECTION, SWINE, CONJUNCTIVITIS, PREVALENCE, PSITTACI, PIGLETS, STRAIN
journal title
MICROSCOPY RESEARCH AND TECHNIQUE
Microsc. Res. Tech.
volume
80
issue
4
pages
350 - 356
Web of Science type
Article
Web of Science id
000397853400004
ISSN
1059-910X
DOI
10.1002/jemt.22799
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8506862
handle
http://hdl.handle.net/1854/LU-8506862
date created
2017-02-02 10:05:07
date last changed
2017-09-19 11:42:04
@article{8506862,
  abstract     = {The porcine pathogen Chlamydia suis is widespread in pig farming. Isolation of Chlamydia suis in cell culture is crucial for the generation and characterization of new isolates. However, isolation of Chlamydia suis strains from field samples is fastidious. Therefore, we exploited high-content microscopy to quantify the growth of Chlamydia suis strains in different cell lines. We found that the cell line yielding optimal propagation of Chlamydia suis differed among isolates, and we identified cell lines outperforming those routinely used for chlamydial isolation. We conclude that adaptation of the propagation procedure to the origin of the putative field isolate is highly recommended to improve the recovery rate.},
  author       = {De Puysseleyr, Leentje and De Puysseleyr, Kristien and Vanrompay, Daisy and De Vos, Winnok},
  issn         = {1059-910X},
  journal      = {MICROSCOPY RESEARCH AND TECHNIQUE},
  keyword      = {Chlamydia,cell culture,high-content microscopy,isolation,swine,GNOTOBIOTIC PIGS,REPRODUCTIVE FAILURE,IN-VITRO,INFECTION,SWINE,CONJUNCTIVITIS,PREVALENCE,PSITTACI,PIGLETS,STRAIN},
  language     = {eng},
  number       = {4},
  pages        = {350--356},
  title        = {Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy},
  url          = {http://dx.doi.org/10.1002/jemt.22799},
  volume       = {80},
  year         = {2017},
}

Chicago
De Puysseleyr, Leentje, Kristien De Puysseleyr, Daisy Vanrompay, and Winnok De Vos. 2017. “Quantifying the Growth of Chlamydia Suis in Cell Culture Using High-content Microscopy.” Microscopy Research and Technique 80 (4): 350–356.
APA
De Puysseleyr, L., De Puysseleyr, K., Vanrompay, D., & De Vos, W. (2017). Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy. MICROSCOPY RESEARCH AND TECHNIQUE, 80(4), 350–356.
Vancouver
1.
De Puysseleyr L, De Puysseleyr K, Vanrompay D, De Vos W. Quantifying the growth of Chlamydia suis in cell culture using high-content microscopy. MICROSCOPY RESEARCH AND TECHNIQUE. 2017;80(4):350–6.
MLA
De Puysseleyr, Leentje, Kristien De Puysseleyr, Daisy Vanrompay, et al. “Quantifying the Growth of Chlamydia Suis in Cell Culture Using High-content Microscopy.” MICROSCOPY RESEARCH AND TECHNIQUE 80.4 (2017): 350–356. Print.