Laser-assisted photoporation : fundamentals, technological advances and applications
- Author
- Ranhua Xiong (UGent) , Sangram Keshari Samal (UGent) , Jo Demeester (UGent) , Andre Skirtach (UGent) , Stefaan De Smedt (UGent) and Kevin Braeckmans (UGent)
- Organization
- Project
- Abstract
- Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-of-the-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticle-mediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future.
- Keywords
- Photoporation, laser, intracellular delivery, gold nanoparticles, carbon nanomaterials, RED-BLOOD-CELLS, FEMTOSECOND LASER, GOLD NANOPARTICLES, MAMMALIAN-CELLS, IN-VIVO, GENE-TRANSFER, LIVE CELLS, INTRACELLULAR DELIVERY, PLASMONIC NANOBUBBLES, OPTICAL-INJECTION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8506681
- MLA
- Xiong, Ranhua, et al. “Laser-Assisted Photoporation : Fundamentals, Technological Advances and Applications.” ADVANCES IN PHYSICS-X, vol. 1, no. 4, 2016, pp. 596–620, doi:10.1080/23746149.2016.1228476.
- APA
- Xiong, R., Samal, S. K., Demeester, J., Skirtach, A., De Smedt, S., & Braeckmans, K. (2016). Laser-assisted photoporation : fundamentals, technological advances and applications. ADVANCES IN PHYSICS-X, 1(4), 596–620. https://doi.org/10.1080/23746149.2016.1228476
- Chicago author-date
- Xiong, Ranhua, Sangram Keshari Samal, Jo Demeester, Andre Skirtach, Stefaan De Smedt, and Kevin Braeckmans. 2016. “Laser-Assisted Photoporation : Fundamentals, Technological Advances and Applications.” ADVANCES IN PHYSICS-X 1 (4): 596–620. https://doi.org/10.1080/23746149.2016.1228476.
- Chicago author-date (all authors)
- Xiong, Ranhua, Sangram Keshari Samal, Jo Demeester, Andre Skirtach, Stefaan De Smedt, and Kevin Braeckmans. 2016. “Laser-Assisted Photoporation : Fundamentals, Technological Advances and Applications.” ADVANCES IN PHYSICS-X 1 (4): 596–620. doi:10.1080/23746149.2016.1228476.
- Vancouver
- 1.Xiong R, Samal SK, Demeester J, Skirtach A, De Smedt S, Braeckmans K. Laser-assisted photoporation : fundamentals, technological advances and applications. ADVANCES IN PHYSICS-X. 2016;1(4):596–620.
- IEEE
- [1]R. Xiong, S. K. Samal, J. Demeester, A. Skirtach, S. De Smedt, and K. Braeckmans, “Laser-assisted photoporation : fundamentals, technological advances and applications,” ADVANCES IN PHYSICS-X, vol. 1, no. 4, pp. 596–620, 2016.
@article{8506681,
abstract = {{Laser-assisted photoporation is a promising technique that is receiving increasing attention for the delivery of membrane impermeable nanoscopic substances into living cells. Photoporation is based on the generation of localized transient pores in the cell membrane using continuous or pulsed laser light. Increased membrane permeability can be achieved directly by focused laser light or in combination with sensitizing nanoparticles for higher throughput. Here, we provide a detailed account on the history and current state-of-the-art of photoporation as a physical nanomaterial delivery technique. We first introduce with a detailed explanation of the mechanisms responsible for cell membrane pore formation, following an overview of experimental procedures for realizing direct laser photoporation. Next, we review the second and most recent method of photoporation that combines laser light with sensitizing NPs. The different mechanisms of pore formation are discussed and an overview is given of the various types of sensitizing nanomaterials. Typical experimental setups to achieve nanoparticle-mediated photoporation are discussed as well. Finally, we discuss the biological and therapeutic applications enabled by photoporation and give our current view on this expanding research field and the challenges and opportunities that remain for the near future.}},
author = {{Xiong, Ranhua and Samal, Sangram Keshari and Demeester, Jo and Skirtach, Andre and De Smedt, Stefaan and Braeckmans, Kevin}},
issn = {{2374-6149}},
journal = {{ADVANCES IN PHYSICS-X}},
keywords = {{Photoporation,laser,intracellular delivery,gold nanoparticles,carbon nanomaterials,RED-BLOOD-CELLS,FEMTOSECOND LASER,GOLD NANOPARTICLES,MAMMALIAN-CELLS,IN-VIVO,GENE-TRANSFER,LIVE CELLS,INTRACELLULAR DELIVERY,PLASMONIC NANOBUBBLES,OPTICAL-INJECTION}},
language = {{eng}},
number = {{4}},
pages = {{596--620}},
title = {{Laser-assisted photoporation : fundamentals, technological advances and applications}},
url = {{http://doi.org/10.1080/23746149.2016.1228476}},
volume = {{1}},
year = {{2016}},
}
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