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Development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies

(2016) ANTIBODIES. 5(2).
Author
Organization
Abstract
Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn+) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermore, sialic acid-binding of red blood cells (RBC) could be inhibited with mAbs recognizing different epitopes and all mAb showed internalization of Sn. The newly developed mAbs can be used as novel tools for Sn research and further analysis of Sn internalization in different species.
Keywords
sialoadhesin, monoclonal antibody, internalization, RESPIRATORY SYNDROME VIRUS, ACID-BINDING-RECEPTOR, PORCINE ARTERIVIRUS INFECTION, I-TYPE LECTINS, SIALIC-ACID, ALVEOLAR MACROPHAGES, DENDRITIC CELLS, IMMUNE-SYSTEM, LYMPH-NODES, B-CELLS

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Citation

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MLA
De Schryver, Marjorie, Hanne Van Gorp, Inge Hoebeke, et al. “Development and Characterization of New Species Cross-reactive Anti-sialoadhesin Monoclonal Antibodies.” ANTIBODIES 5.2 (2016): n. pag. Print.
APA
De Schryver, Marjorie, Van Gorp, H., Hoebeke, I., De Maeyer, B., Ooms, K., Pintelon, I., Maes, L. J., et al. (2016). Development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies. ANTIBODIES, 5(2).
Chicago author-date
De Schryver, Marjorie, Hanne Van Gorp, Inge Hoebeke, Bauke De Maeyer, Karen Ooms, Isabel Pintelon, Louis J Maes, Paul Cos, Hans Nauwynck, and Peter L Delputte. 2016. “Development and Characterization of New Species Cross-reactive Anti-sialoadhesin Monoclonal Antibodies.” Antibodies 5 (2).
Chicago author-date (all authors)
De Schryver, Marjorie, Hanne Van Gorp, Inge Hoebeke, Bauke De Maeyer, Karen Ooms, Isabel Pintelon, Louis J Maes, Paul Cos, Hans Nauwynck, and Peter L Delputte. 2016. “Development and Characterization of New Species Cross-reactive Anti-sialoadhesin Monoclonal Antibodies.” Antibodies 5 (2).
Vancouver
1.
De Schryver M, Van Gorp H, Hoebeke I, De Maeyer B, Ooms K, Pintelon I, et al. Development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies. ANTIBODIES. 2016;5(2).
IEEE
[1]
M. De Schryver et al., “Development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies,” ANTIBODIES, vol. 5, no. 2, 2016.
@article{8506068,
  abstract     = {Sialoadhesin (Sn) is a surface receptor expressed on a subset of macrophages in steady state conditions. During inflammation and diseases, Sn is highly upregulated on macrophages and blood monocytes. Therefore, therapies using monoclonal antibodies (mAbs) to target Sn-positive (Sn+) cells are a potential strategy for targeted treatment. It has been shown that Sn internalizes after binding with a mAb, though it is not clear whether this is species-specific. In this study, new Sn-specific mAbs were developed and analyzed for cross-reactivity between species. In addition, the newly developed mAbs were compared to mAbs used in previous research for their epitope recognition and other Sn-specific characteristics. Both species-specific and cross-reactive antibodies could be identified. Furthermore, sialic acid-binding of red blood cells (RBC) could be inhibited with mAbs recognizing different epitopes and all mAb showed internalization of Sn. The newly developed mAbs can be used as novel tools for Sn research and further analysis of Sn internalization in different species.},
  articleno    = {7},
  author       = {De Schryver, Marjorie and Van Gorp, Hanne and Hoebeke, Inge and De Maeyer, Bauke and Ooms, Karen and Pintelon, Isabel and Maes, Louis J and Cos, Paul and Nauwynck, Hans and Delputte, Peter L},
  issn         = {2073-4468},
  journal      = {ANTIBODIES},
  keywords     = {sialoadhesin,monoclonal antibody,internalization,RESPIRATORY SYNDROME VIRUS,ACID-BINDING-RECEPTOR,PORCINE ARTERIVIRUS INFECTION,I-TYPE LECTINS,SIALIC-ACID,ALVEOLAR MACROPHAGES,DENDRITIC CELLS,IMMUNE-SYSTEM,LYMPH-NODES,B-CELLS},
  language     = {eng},
  number       = {2},
  pages        = {18},
  title        = {Development and characterization of new species cross-reactive anti-sialoadhesin monoclonal antibodies},
  url          = {http://dx.doi.org/10.3390/antib5020007},
  volume       = {5},
  year         = {2016},
}

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