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Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis

Pavel Kerchev, Cezary Waszczak UGent, Aleksandra Lewandowska UGent, Patrick Willems UGent, Alexey Shapiguzov, Zhen Li UGent, Saleh Alseekh, Per Mühlenbock UGent, Frank Hoeberichts UGent, Jingjing Huang, et al. (2016) PLANT PHYSIOLOGY. 171(3). p.1704-1719
abstract
The genes coding for the core metabolic enzymes of the photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered in genetic screens aimed to isolate mutants that are unviable under ambient air. As an exception, glycolate oxidase (GOX) mutants with a photorespiratory phenotype have not been described yet in C3 species. Using Arabidopsis (Arabidopsis thaliana) mutants lacking the peroxisomal CATALASE2 (cat2-2) that display stunted growth and cell death lesions under ambient air, we isolated a second-site loss-of-function mutation in GLYCOLATE OXIDASE1 (GOX1) that attenuated the photorespiratory phenotype of cat2-2. Interestingly, knocking out the nearly identical GOX2 in the cat2-2 background did not affect the photorespiratory phenotype, indicating that GOX1 and GOX2 play distinct metabolic roles. We further investigated their individual functions in single gox1-1 and gox2-1 mutants and revealed that their phenotypes can be modulated by environmental conditions that increase the metabolic flux through the photorespiratory pathway. High light negatively affected the photosynthetic performance and growth of both gox1-1 and gox2-1 mutants, but the negative consequences of severe photorespiration were more pronounced in the absence of GOX1, which was accompanied with lesser ability to process glycolate. Taken together, our results point toward divergent functions of the two photorespiratory GOX isoforms in Arabidopsis and contribute to a better understanding of the photorespiratory pathway.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
S-NITROSYLATED PROTEINS, OXYGEN GENE NETWORK, ASCORBATE PEROXIDASE, CELL-DEATH, MAXIMUM-LIKELIHOOD, OXIDATIVE STRESS, CLIMATE-CHANGE, PLANT, METABOLISM, PROTECTION
journal title
PLANT PHYSIOLOGY
Plant Physiol.
volume
171
issue
3
pages
1704 - 1719
Web of Science type
Article
Web of Science id
000381303300017
JCR category
PLANT SCIENCES
JCR impact factor
6.456 (2016)
JCR rank
11/211 (2016)
JCR quartile
1 (2016)
ISSN
0032-0889
DOI
10.1104/pp.16.00359
project
Bioinformatics: from nucleotids to networks (N2N)
project
Biotechnology for a sustainable economy (Bio-Economy)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8504161
handle
http://hdl.handle.net/1854/LU-8504161
date created
2017-01-24 14:45:01
date last changed
2017-02-24 12:55:04
@article{8504161,
  abstract     = {The genes coding for the core metabolic enzymes of the photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered in genetic screens aimed to isolate mutants that are unviable under ambient air. As an exception, glycolate oxidase (GOX) mutants with a photorespiratory phenotype have not been described yet in C3 species. Using Arabidopsis (Arabidopsis thaliana) mutants lacking the peroxisomal CATALASE2 (cat2-2) that display stunted growth and cell death lesions under ambient air, we isolated a second-site loss-of-function mutation in GLYCOLATE OXIDASE1 (GOX1) that attenuated the photorespiratory phenotype of cat2-2. Interestingly, knocking out the nearly identical GOX2 in the cat2-2 background did not affect the photorespiratory phenotype, indicating that GOX1 and GOX2 play distinct metabolic roles. We further investigated their individual functions in single gox1-1 and gox2-1 mutants and revealed that their phenotypes can be modulated by environmental conditions that increase the metabolic flux through the photorespiratory pathway. High light negatively affected the photosynthetic performance and growth of both gox1-1 and gox2-1 mutants, but the negative consequences of severe photorespiration were more pronounced in the absence of GOX1, which was accompanied with lesser ability to process glycolate. Taken together, our results point toward divergent functions of the two photorespiratory GOX isoforms in Arabidopsis and contribute to a better understanding of the photorespiratory pathway.},
  author       = {Kerchev, Pavel and Waszczak, Cezary and Lewandowska, Aleksandra and Willems, Patrick and Shapiguzov, Alexey and Li, Zhen and Alseekh, Saleh and M{\"u}hlenbock, Per and Hoeberichts, Frank and Huang, Jingjing and Van Der Kelen, Katrien and Kangasjarvi, Jaakko and Fernie, Alisdair R and De Smet, Riet and Van de Peer, Yves and Messens, Joris and Van Breusegem, Frank},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {S-NITROSYLATED PROTEINS,OXYGEN GENE NETWORK,ASCORBATE PEROXIDASE,CELL-DEATH,MAXIMUM-LIKELIHOOD,OXIDATIVE STRESS,CLIMATE-CHANGE,PLANT,METABOLISM,PROTECTION},
  language     = {eng},
  number       = {3},
  pages        = {1704--1719},
  title        = {Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis},
  url          = {http://dx.doi.org/10.1104/pp.16.00359},
  volume       = {171},
  year         = {2016},
}

Chicago
Kerchev, Pavel, Cezary Waszczak, Aleksandra Lewandowska, Patrick Willems, Alexey Shapiguzov, Zhen Li, Saleh Alseekh, et al. 2016. “Lack of GLYCOLATE OXIDASE1, but Not GLYCOLATE OXIDASE2, Attenuates the Photorespiratory Phenotype of CATALASE2-deficient Arabidopsis.” Plant Physiology 171 (3): 1704–1719.
APA
Kerchev, P., Waszczak, C., Lewandowska, A., Willems, P., Shapiguzov, A., Li, Z., Alseekh, S., et al. (2016). Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis. PLANT PHYSIOLOGY, 171(3), 1704–1719.
Vancouver
1.
Kerchev P, Waszczak C, Lewandowska A, Willems P, Shapiguzov A, Li Z, et al. Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis. PLANT PHYSIOLOGY. 2016;171(3):1704–19.
MLA
Kerchev, Pavel, Cezary Waszczak, Aleksandra Lewandowska, et al. “Lack of GLYCOLATE OXIDASE1, but Not GLYCOLATE OXIDASE2, Attenuates the Photorespiratory Phenotype of CATALASE2-deficient Arabidopsis.” PLANT PHYSIOLOGY 171.3 (2016): 1704–1719. Print.