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Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis

Pavel Kerchev (UGent) , Cezary Waszczak (UGent) , Aleksandra Lewandowska (UGent) , Patrick Willems (UGent) , Alexey Shapiguzov, Zhen Li (UGent) , Saleh Alseekh, Per Mühlenbock (UGent) , Frank Hoeberichts (UGent) , Jingjing Huang, et al.
(2016) PLANT PHYSIOLOGY. 171(3). p.1704-1719
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Abstract
The genes coding for the core metabolic enzymes of the photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered in genetic screens aimed to isolate mutants that are unviable under ambient air. As an exception, glycolate oxidase (GOX) mutants with a photorespiratory phenotype have not been described yet in C3 species. Using Arabidopsis (Arabidopsis thaliana) mutants lacking the peroxisomal CATALASE2 (cat2-2) that display stunted growth and cell death lesions under ambient air, we isolated a second-site loss-of-function mutation in GLYCOLATE OXIDASE1 (GOX1) that attenuated the photorespiratory phenotype of cat2-2. Interestingly, knocking out the nearly identical GOX2 in the cat2-2 background did not affect the photorespiratory phenotype, indicating that GOX1 and GOX2 play distinct metabolic roles. We further investigated their individual functions in single gox1-1 and gox2-1 mutants and revealed that their phenotypes can be modulated by environmental conditions that increase the metabolic flux through the photorespiratory pathway. High light negatively affected the photosynthetic performance and growth of both gox1-1 and gox2-1 mutants, but the negative consequences of severe photorespiration were more pronounced in the absence of GOX1, which was accompanied with lesser ability to process glycolate. Taken together, our results point toward divergent functions of the two photorespiratory GOX isoforms in Arabidopsis and contribute to a better understanding of the photorespiratory pathway.
Keywords
S-NITROSYLATED PROTEINS, OXYGEN GENE NETWORK, ASCORBATE PEROXIDASE, CELL-DEATH, MAXIMUM-LIKELIHOOD, OXIDATIVE STRESS, CLIMATE-CHANGE, PLANT, METABOLISM, PROTECTION

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MLA
Kerchev, Pavel, et al. “Lack of GLYCOLATE OXIDASE1, but Not GLYCOLATE OXIDASE2, Attenuates the Photorespiratory Phenotype of CATALASE2-Deficient Arabidopsis.” PLANT PHYSIOLOGY, vol. 171, no. 3, 2016, pp. 1704–19, doi:10.1104/pp.16.00359.
APA
Kerchev, P., Waszczak, C., Lewandowska, A., Willems, P., Shapiguzov, A., Li, Z., … Van Breusegem, F. (2016). Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis. PLANT PHYSIOLOGY, 171(3), 1704–1719. https://doi.org/10.1104/pp.16.00359
Chicago author-date
Kerchev, Pavel, Cezary Waszczak, Aleksandra Lewandowska, Patrick Willems, Alexey Shapiguzov, Zhen Li, Saleh Alseekh, et al. 2016. “Lack of GLYCOLATE OXIDASE1, but Not GLYCOLATE OXIDASE2, Attenuates the Photorespiratory Phenotype of CATALASE2-Deficient Arabidopsis.” PLANT PHYSIOLOGY 171 (3): 1704–19. https://doi.org/10.1104/pp.16.00359.
Chicago author-date (all authors)
Kerchev, Pavel, Cezary Waszczak, Aleksandra Lewandowska, Patrick Willems, Alexey Shapiguzov, Zhen Li, Saleh Alseekh, Per Mühlenbock, Frank Hoeberichts, Jingjing Huang, Katrien Van Der Kelen, Jaakko Kangasjarvi, Alisdair R Fernie, Riet De Smet, Yves Van de Peer, Joris Messens, and Frank Van Breusegem. 2016. “Lack of GLYCOLATE OXIDASE1, but Not GLYCOLATE OXIDASE2, Attenuates the Photorespiratory Phenotype of CATALASE2-Deficient Arabidopsis.” PLANT PHYSIOLOGY 171 (3): 1704–1719. doi:10.1104/pp.16.00359.
Vancouver
1.
Kerchev P, Waszczak C, Lewandowska A, Willems P, Shapiguzov A, Li Z, et al. Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis. PLANT PHYSIOLOGY. 2016;171(3):1704–19.
IEEE
[1]
P. Kerchev et al., “Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis,” PLANT PHYSIOLOGY, vol. 171, no. 3, pp. 1704–1719, 2016.
@article{8504161,
  abstract     = {{The genes coding for the core metabolic enzymes of the photorespiratory pathway that allows plants with C3-type photosynthesis to survive in an oxygen-rich atmosphere, have been largely discovered in genetic screens aimed to isolate mutants that are unviable under ambient air. As an exception, glycolate oxidase (GOX) mutants with a photorespiratory phenotype have not been described yet in C3 species. Using Arabidopsis (Arabidopsis thaliana) mutants lacking the peroxisomal CATALASE2 (cat2-2) that display stunted growth and cell death lesions under ambient air, we isolated a second-site loss-of-function mutation in GLYCOLATE OXIDASE1 (GOX1) that attenuated the photorespiratory phenotype of cat2-2. Interestingly, knocking out the nearly identical GOX2 in the cat2-2 background did not affect the photorespiratory phenotype, indicating that GOX1 and GOX2 play distinct metabolic roles. We further investigated their individual functions in single gox1-1 and gox2-1 mutants and revealed that their phenotypes can be modulated by environmental conditions that increase the metabolic flux through the photorespiratory pathway. High light negatively affected the photosynthetic performance and growth of both gox1-1 and gox2-1 mutants, but the negative consequences of severe photorespiration were more pronounced in the absence of GOX1, which was accompanied with lesser ability to process glycolate. Taken together, our results point toward divergent functions of the two photorespiratory GOX isoforms in Arabidopsis and contribute to a better understanding of the photorespiratory pathway.}},
  author       = {{Kerchev, Pavel and Waszczak, Cezary and Lewandowska, Aleksandra and Willems, Patrick and Shapiguzov, Alexey and Li, Zhen and Alseekh, Saleh and Mühlenbock, Per and Hoeberichts, Frank and Huang, Jingjing and Van Der Kelen, Katrien and Kangasjarvi, Jaakko and Fernie, Alisdair R and De Smet, Riet and Van de Peer, Yves and Messens, Joris and Van Breusegem, Frank}},
  issn         = {{0032-0889}},
  journal      = {{PLANT PHYSIOLOGY}},
  keywords     = {{S-NITROSYLATED PROTEINS,OXYGEN GENE NETWORK,ASCORBATE PEROXIDASE,CELL-DEATH,MAXIMUM-LIKELIHOOD,OXIDATIVE STRESS,CLIMATE-CHANGE,PLANT,METABOLISM,PROTECTION}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{1704--1719}},
  title        = {{Lack of GLYCOLATE OXIDASE1, but not GLYCOLATE OXIDASE2, attenuates the photorespiratory phenotype of CATALASE2-deficient Arabidopsis}},
  url          = {{http://doi.org/10.1104/pp.16.00359}},
  volume       = {{171}},
  year         = {{2016}},
}
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