Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum
- Author
- Tomasz Walski (UGent) , Els Van Damme (UGent) , Nicolas Smargiasso, Olivier Christiaens (UGent) , Edwin De Pauw and Guy Smagghe (UGent)
- Organization
- Abstract
- In holometabolous insects the transition from larva to adult requires a complete body reorganization and relies on N-glycosylated proteins. N-glycosylation is an important posttranslational modification that influences protein activity but its impact on the metamorphosis has not been studied yet. Here we used the red flour beetle, Tribolium castaneum, to perform a first comprehensive study on the involvement of the protein N-glycosylation pathway in metamorphosis. The transcript levels for genes encoding N-glycan processing enzymes increased during later developmental stages and, in turn, transition from larva to adult coincided with an enrichment of more extensively modified paucimannose glycans, including fucosylated ones. Blockage of N-glycan attachment resulted in larval mortality, while RNAi of alpha-glucosidases involved in early N-glycan trimming and quality control disrupted the larva to pupa transition. Additionally, simultaneous knockdown of multiple genes responsible for N-glycan processing towards paucimannose structures revealed their novel roles in pupal appendage formation and adult eclosion. Our findings revealed that, next to hormonal control, insect post-embryonic development and metamorphosis depend on protein N-glycan attachment and efficient N-glycan processing. Consequently, disruption of these processes could be an effective new approach for insect control.
- Keywords
- RED FLOUR BEETLE, DROSOPHILA-MELANOGASTER, CAENORHABDITIS-ELEGANS, SUBSTRATE-SPECIFICITY, INSECT CELLS, ENDOPLASMIC-RETICULUM, FUNCTIONAL-ANALYSIS, MASS-SPECTROMETRY, ALPHA-MANNOSIDASE, JUVENILE-HORMONE
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8503132
- MLA
- Walski, Tomasz, et al. “Protein N-Glycosylation and N-Glycan Trimming Are Required for Postembryonic Development of the Pest Beetle Tribolium Castaneum.” SCIENTIFIC REPORTS, vol. 6, 2016, doi:10.1038/srep35151.
- APA
- Walski, T., Van Damme, E., Smargiasso, N., Christiaens, O., De Pauw, E., & Smagghe, G. (2016). Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum. SCIENTIFIC REPORTS, 6. https://doi.org/10.1038/srep35151
- Chicago author-date
- Walski, Tomasz, Els Van Damme, Nicolas Smargiasso, Olivier Christiaens, Edwin De Pauw, and Guy Smagghe. 2016. “Protein N-Glycosylation and N-Glycan Trimming Are Required for Postembryonic Development of the Pest Beetle Tribolium Castaneum.” SCIENTIFIC REPORTS 6. https://doi.org/10.1038/srep35151.
- Chicago author-date (all authors)
- Walski, Tomasz, Els Van Damme, Nicolas Smargiasso, Olivier Christiaens, Edwin De Pauw, and Guy Smagghe. 2016. “Protein N-Glycosylation and N-Glycan Trimming Are Required for Postembryonic Development of the Pest Beetle Tribolium Castaneum.” SCIENTIFIC REPORTS 6. doi:10.1038/srep35151.
- Vancouver
- 1.Walski T, Van Damme E, Smargiasso N, Christiaens O, De Pauw E, Smagghe G. Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum. SCIENTIFIC REPORTS. 2016;6.
- IEEE
- [1]T. Walski, E. Van Damme, N. Smargiasso, O. Christiaens, E. De Pauw, and G. Smagghe, “Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum,” SCIENTIFIC REPORTS, vol. 6, 2016.
@article{8503132,
abstract = {{In holometabolous insects the transition from larva to adult requires a complete body reorganization and relies on N-glycosylated proteins. N-glycosylation is an important posttranslational modification that influences protein activity but its impact on the metamorphosis has not been studied yet. Here we used the red flour beetle, Tribolium castaneum, to perform a first comprehensive study on the involvement of the protein N-glycosylation pathway in metamorphosis. The transcript levels for genes encoding N-glycan processing enzymes increased during later developmental stages and, in turn, transition from larva to adult coincided with an enrichment of more extensively modified paucimannose glycans, including fucosylated ones. Blockage of N-glycan attachment resulted in larval mortality, while RNAi of alpha-glucosidases involved in early N-glycan trimming and quality control disrupted the larva to pupa transition. Additionally, simultaneous knockdown of multiple genes responsible for N-glycan processing towards paucimannose structures revealed their novel roles in pupal appendage formation and adult eclosion. Our findings revealed that, next to hormonal control, insect post-embryonic development and metamorphosis depend on protein N-glycan attachment and efficient N-glycan processing. Consequently, disruption of these processes could be an effective new approach for insect control.}},
articleno = {{35151}},
author = {{Walski, Tomasz and Van Damme, Els and Smargiasso, Nicolas and Christiaens, Olivier and De Pauw, Edwin and Smagghe, Guy}},
issn = {{2045-2322}},
journal = {{SCIENTIFIC REPORTS}},
keywords = {{RED FLOUR BEETLE,DROSOPHILA-MELANOGASTER,CAENORHABDITIS-ELEGANS,SUBSTRATE-SPECIFICITY,INSECT CELLS,ENDOPLASMIC-RETICULUM,FUNCTIONAL-ANALYSIS,MASS-SPECTROMETRY,ALPHA-MANNOSIDASE,JUVENILE-HORMONE}},
language = {{eng}},
pages = {{15}},
title = {{Protein N-glycosylation and N-glycan trimming are required for postembryonic development of the pest beetle Tribolium castaneum}},
url = {{http://dx.doi.org/10.1038/srep35151}},
volume = {{6}},
year = {{2016}},
}
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