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Whole-genome sequence analysis of Bombella intestini LMG 28161T, a novel acetic acid bacterium isolated from the crop of a red-tailed bumble bee, Bombus lapidarius

Leilei Li, Koen Illeghems, Simon Van Kerrebroeck, Wim Borremans, Ilse Cleenwerck UGent, Guy Smagghe UGent, Luc De Vuyst and Peter Vandamme UGent (2016) PLOS ONE. 11(11).
abstract
The whole-genome sequence of Bombella intestini LMG 28161(T), an endosymbiotic acetic acid bacterium (AAB) occurring in bumble bees, was determined to investigate the molecular mechanisms underlying its metabolic capabilities. The draft genome sequence of B. intestini LMG 28161(T) was 2.02 Mb. Metabolic carbohydrate pathways were in agreement with the metabolite analyses of fermentation experiments and revealed its oxidative capacity towards sucrose, D-glucose, D-fructose and D-mannitol, but not ethanol and glycerol. The results of the fermentation experiments also demonstrated that the lack of effective aeration in small-scale carbohydrate consumption experiments may be responsible for the lack of reproducibility of such results in taxonomic studies of AAB. Finally, compared to the genome sequences of its nearest phylogenetic neighbor and of three other insect associated AAB strains, the B. intestini LMG 28161(T) genome lost 69 orthologs and included 89 unique genes. Although many of the latter were hypothetical they also included several type IV secretion system proteins, amino acid transporter/permeases and membrane proteins which might play a role in the interaction with the bumble bee host.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
COCOA BEAN FERMENTATION, IV SECRETION SYSTEM, ACETOBACTER-PASTEURIANUS, HONEY-BEE, SP-NOV., GLUCONOBACTER-OXYDANS, GEN. NOV., IDENTIFICATION, SYMBIONTS, EVOLUTION
journal title
PLOS ONE
PLoS One
volume
11
issue
11
article number
e0165611
Web of Science type
Article
Web of Science id
000387909300022
JCR category
MULTIDISCIPLINARY SCIENCES
JCR impact factor
2.806 (2016)
JCR rank
15/64 (2016)
JCR quartile
1 (2016)
ISSN
1932-6203
DOI
10.1371/journal.pone.0165611
language
English
UGent publication?
yes
classification
A1
copyright statement
I have retained and own the full copyright for this publication
id
8503130
handle
http://hdl.handle.net/1854/LU-8503130
date created
2017-01-20 10:54:11
date last changed
2017-02-10 11:05:14
@article{8503130,
  abstract     = {The whole-genome sequence of Bombella intestini LMG 28161(T), an endosymbiotic acetic acid bacterium (AAB) occurring in bumble bees, was determined to investigate the molecular mechanisms underlying its metabolic capabilities. The draft genome sequence of B. intestini LMG 28161(T) was 2.02 Mb. Metabolic carbohydrate pathways were in agreement with the metabolite analyses of fermentation experiments and revealed its oxidative capacity towards sucrose, D-glucose, D-fructose and D-mannitol, but not ethanol and glycerol. The results of the fermentation experiments also demonstrated that the lack of effective aeration in small-scale carbohydrate consumption experiments may be responsible for the lack of reproducibility of such results in taxonomic studies of AAB. Finally, compared to the genome sequences of its nearest phylogenetic neighbor and of three other insect associated AAB strains, the B. intestini LMG 28161(T) genome lost 69 orthologs and included 89 unique genes. Although many of the latter were hypothetical they also included several type IV secretion system proteins, amino acid transporter/permeases and membrane proteins which might play a role in the interaction with the bumble bee host.},
  articleno    = {e0165611},
  author       = {Li, Leilei and Illeghems, Koen and Van Kerrebroeck, Simon and Borremans, Wim and Cleenwerck, Ilse and Smagghe, Guy and De Vuyst, Luc and Vandamme, Peter},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keyword      = {COCOA BEAN FERMENTATION,IV SECRETION SYSTEM,ACETOBACTER-PASTEURIANUS,HONEY-BEE,SP-NOV.,GLUCONOBACTER-OXYDANS,GEN. NOV.,IDENTIFICATION,SYMBIONTS,EVOLUTION},
  language     = {eng},
  number       = {11},
  title        = {Whole-genome sequence analysis of Bombella intestini LMG 28161T, a novel acetic acid bacterium isolated from the crop of a red-tailed bumble bee, Bombus lapidarius},
  url          = {http://dx.doi.org/10.1371/journal.pone.0165611},
  volume       = {11},
  year         = {2016},
}

Chicago
Li, Leilei, Koen Illeghems, Simon Van Kerrebroeck, Wim Borremans, Ilse Cleenwerck, Guy Smagghe, Luc De Vuyst, and Peter Vandamme. 2016. “Whole-genome Sequence Analysis of Bombella Intestini LMG 28161T, a Novel Acetic Acid Bacterium Isolated from the Crop of a Red-tailed Bumble Bee, Bombus Lapidarius.” Plos One 11 (11).
APA
Li, Leilei, Illeghems, K., Van Kerrebroeck, S., Borremans, W., Cleenwerck, I., Smagghe, G., De Vuyst, L., et al. (2016). Whole-genome sequence analysis of Bombella intestini LMG 28161T, a novel acetic acid bacterium isolated from the crop of a red-tailed bumble bee, Bombus lapidarius. PLOS ONE, 11(11).
Vancouver
1.
Li L, Illeghems K, Van Kerrebroeck S, Borremans W, Cleenwerck I, Smagghe G, et al. Whole-genome sequence analysis of Bombella intestini LMG 28161T, a novel acetic acid bacterium isolated from the crop of a red-tailed bumble bee, Bombus lapidarius. PLOS ONE. 2016;11(11).
MLA
Li, Leilei, Koen Illeghems, Simon Van Kerrebroeck, et al. “Whole-genome Sequence Analysis of Bombella Intestini LMG 28161T, a Novel Acetic Acid Bacterium Isolated from the Crop of a Red-tailed Bumble Bee, Bombus Lapidarius.” PLOS ONE 11.11 (2016): n. pag. Print.