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Haemophilus influenzae biofilm formation in chronic otitis media with effusion

Helen Van Hoecke UGent, Ann-Sophie De Paepe, Edward Lambert, Jonas Van Belleghem UGent, Piet Cools, Leen Van Simaey UGent, Pieter Deschaght UGent, Mario Vaneechoutte UGent and Ingeborg Dhooge UGent (2016) EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY. 273(11). p.3553-3560
abstract
Otitis media with effusion (OME) is a highly prevalent disease in children, but the exact pathogenesis and role of bacteria are still not well understood. This study aimed to investigate the presence of otopathogenic bacteria in the middle ear effusion (MEE) and adenoid of children with chronic OME (COME), and to investigate in vivo whether these bacteria, especially Haemophilus influenzae, are organized as a biofilm in the middle ear fluid. MEE and adenoid samples were collected from 21 patients with COME. Extensive bacterial culturing and genotyping was performed on all middle ear and adenoid samples. Fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) was used to visualize possible biofilm structures for a selection of middle ear effusion samples. 34 MEE samples were collected from 21 patients of which 64.7 % were culture positive for bacteria and 47.0 % were culture positive for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and/or Streptococcus pneumoniae. All 21 adenoid samples were culture positive for one or more of these four otopathogens. H. influenzae (35.3 %) and S. pneumoniae (76.2 %) were the most frequently cultured bacteria in the MEE and adenoid samples, respectively. The same bacterial species was found in MEE and adenoid for 84.6 % of the patients and in 81.2 % of the cases where the same species was found in more than one site it involved the same bacterial genotype. FISH and CLSM demonstrated the presence of H. influenzae specific biofilm structures in five of the eight culture positive MEEs that were tested, but in none of the two culture negative MEEs. The findings in this study indicate that the adenoid acts as a reservoir for bacteria in MEE and confirms that biofilms, in at least half of the cases consisting of H. influenzae, are indeed present in the MEE of children with COME. Biofilms may thus play a crucial role in the pathogenesis of COME, which is important in the understanding of this disease and the development of potential future treatment options.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Chronic otitis media with effusion (COME), Biofilm, Fluorescent in situ hybridization (FISH), Confocal scanning laser microscopy (CLSM), Haemophilus influenzae, MIDDLE-EAR MUCOSA, BACTERIAL BIOFILMS, MICROSCOPY, PATHOGENS, CHILDREN
journal title
EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
Eur. Arch. Oto-Rhino-Laryn.
volume
273
issue
11
pages
3553 - 3560
Web of Science type
Article
Web of Science id
000385202600010
ISBN
0937-4477
DOI
10.1007/s00405-016-3958-9
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8502876
handle
http://hdl.handle.net/1854/LU-8502876
date created
2017-01-19 09:36:48
date last changed
2017-03-17 10:41:57
@article{8502876,
  abstract     = {Otitis media with effusion (OME) is a highly prevalent disease in children, but the exact pathogenesis and role of bacteria are still not well understood. This study aimed to investigate the presence of otopathogenic bacteria in the middle ear effusion (MEE) and adenoid of children with chronic OME (COME), and to investigate in vivo whether these bacteria, especially Haemophilus influenzae, are organized as a biofilm in the middle ear fluid. MEE and adenoid samples were collected from 21 patients with COME. Extensive bacterial culturing and genotyping was performed on all middle ear and adenoid samples. Fluorescence in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM) was used to visualize possible biofilm structures for a selection of middle ear effusion samples. 34 MEE samples were collected from 21 patients of which 64.7 \% were culture positive for bacteria and 47.0 \% were culture positive for Haemophilus influenzae, Moraxella catarrhalis, Staphylococcus aureus and/or Streptococcus pneumoniae. All 21 adenoid samples were culture positive for one or more of these four otopathogens. H. influenzae (35.3 \%) and S. pneumoniae (76.2 \%) were the most frequently cultured bacteria in the MEE and adenoid samples, respectively. The same bacterial species was found in MEE and adenoid for 84.6 \% of the patients and in 81.2 \% of the cases where the same species was found in more than one site it involved the same bacterial genotype. FISH and CLSM demonstrated the presence of H. influenzae specific biofilm structures in five of the eight culture positive MEEs that were tested, but in none of the two culture negative MEEs. The findings in this study indicate that the adenoid acts as a reservoir for bacteria in MEE and confirms that biofilms, in at least half of the cases consisting of H. influenzae, are indeed present in the MEE of children with COME. Biofilms may thus play a crucial role in the pathogenesis of COME, which is important in the understanding of this disease and the development of potential future treatment options.},
  author       = {Van Hoecke, Helen and De Paepe, Ann-Sophie and Lambert, Edward and Van Belleghem, Jonas and Cools, Piet and Van Simaey, Leen and Deschaght, Pieter and Vaneechoutte, Mario and Dhooge, Ingeborg},
  isbn         = {0937-4477},
  journal      = {EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY},
  keyword      = {Chronic otitis media with effusion (COME),Biofilm,Fluorescent in situ hybridization (FISH),Confocal scanning laser microscopy (CLSM),Haemophilus influenzae,MIDDLE-EAR MUCOSA,BACTERIAL BIOFILMS,MICROSCOPY,PATHOGENS,CHILDREN},
  language     = {eng},
  number       = {11},
  pages        = {3553--3560},
  title        = {Haemophilus influenzae biofilm formation in chronic otitis media with effusion},
  url          = {http://dx.doi.org/10.1007/s00405-016-3958-9},
  volume       = {273},
  year         = {2016},
}

Chicago
Van Hoecke, Helen, Ann-Sophie De Paepe, Edward Lambert, Jonas Van Belleghem, Piet Cools, Leen Van Simaey, Pieter Deschaght, Mario Vaneechoutte, and Ingeborg Dhooge. 2016. “Haemophilus Influenzae Biofilm Formation in Chronic Otitis Media with Effusion.” European Archives of Oto-rhino-laryngology 273 (11): 3553–3560.
APA
Van Hoecke, H., De Paepe, A.-S., Lambert, E., Van Belleghem, J., Cools, P., Van Simaey, L., Deschaght, P., et al. (2016). Haemophilus influenzae biofilm formation in chronic otitis media with effusion. EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY, 273(11), 3553–3560.
Vancouver
1.
Van Hoecke H, De Paepe A-S, Lambert E, Van Belleghem J, Cools P, Van Simaey L, et al. Haemophilus influenzae biofilm formation in chronic otitis media with effusion. EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY. 2016;273(11):3553–60.
MLA
Van Hoecke, Helen, Ann-Sophie De Paepe, Edward Lambert, et al. “Haemophilus Influenzae Biofilm Formation in Chronic Otitis Media with Effusion.” EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY 273.11 (2016): 3553–3560. Print.