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Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts

(2016) ONCOTARGET. 7(46). p.75603-75615
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Abstract
In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer.
Keywords
rectal prolapse, COLO320DM, colorectal cancer, orthotopic, mouse model, HUMAN-COLON-CARCINOMA, GROWTH-FACTOR RECEPTOR, NUDE-MICE, ENDOTHELIAL-CELLS, ULCER SYNDROME, IN-VIVO, METASTASIS, INTUSSUSCEPTION, INHIBITION, THERAPY

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Chicago
Tommelein, Joke, Félix Gremonprez, Laurine Verset, Elly De Vlieghere, Glenn Wagemans, Christian Gespach, Tom Boterberg, et al. 2016. “Age and Cellular Context Influence Rectal Prolapse Formation in Mice with Caecal Wall Colorectal Cancer Xenografts.” Oncotarget 7 (46): 75603–75615.
APA
Tommelein, J., Gremonprez, F., Verset, L., De Vlieghere, E., Wagemans, G., Gespach, C., Boterberg, T., et al. (2016). Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts. ONCOTARGET, 7(46), 75603–75615.
Vancouver
1.
Tommelein J, Gremonprez F, Verset L, De Vlieghere E, Wagemans G, Gespach C, et al. Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts. ONCOTARGET. 2016;7(46):75603–15.
MLA
Tommelein, Joke, Félix Gremonprez, Laurine Verset, et al. “Age and Cellular Context Influence Rectal Prolapse Formation in Mice with Caecal Wall Colorectal Cancer Xenografts.” ONCOTARGET 7.46 (2016): 75603–75615. Print.
@article{8502786,
  abstract     = {In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70\% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer.},
  author       = {Tommelein, Joke and Gremonprez, F{\'e}lix and Verset, Laurine and De Vlieghere, Elly and Wagemans, Glenn and Gespach, Christian and Boterberg, Tom and Demetter, Pieter and Ceelen, Wim and Bracke, Marc and De Wever, Olivier},
  issn         = {1949-2553},
  journal      = {ONCOTARGET},
  keyword      = {rectal prolapse,COLO320DM,colorectal cancer,orthotopic,mouse model,HUMAN-COLON-CARCINOMA,GROWTH-FACTOR RECEPTOR,NUDE-MICE,ENDOTHELIAL-CELLS,ULCER SYNDROME,IN-VIVO,METASTASIS,INTUSSUSCEPTION,INHIBITION,THERAPY},
  language     = {eng},
  number       = {46},
  pages        = {75603--75615},
  title        = {Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts},
  url          = {http://dx.doi.org/10.18632/oncotarget.12312},
  volume       = {7},
  year         = {2016},
}

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