Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts
- Author
- Joke Tommelein (UGent) , Félix Gremonprez, Laurine Verset, Elly De Vlieghere (UGent) , Glenn Wagemans, Christian Gespach, Tom Boterberg (UGent) , Pieter Demetter, Wim Ceelen (UGent) , marc bracke and Olivier De Wever (UGent)
- Organization
- Abstract
- In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer.
- Keywords
- rectal prolapse, COLO320DM, colorectal cancer, orthotopic, mouse model, HUMAN-COLON-CARCINOMA, GROWTH-FACTOR RECEPTOR, NUDE-MICE, ENDOTHELIAL-CELLS, ULCER SYNDROME, IN-VIVO, METASTASIS, INTUSSUSCEPTION, INHIBITION, THERAPY
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8502786
- MLA
- Tommelein, Joke, et al. “Age and Cellular Context Influence Rectal Prolapse Formation in Mice with Caecal Wall Colorectal Cancer Xenografts.” ONCOTARGET, vol. 7, no. 46, 2016, pp. 75603–15, doi:10.18632/oncotarget.12312.
- APA
- Tommelein, J., Gremonprez, F., Verset, L., De Vlieghere, E., Wagemans, G., Gespach, C., … De Wever, O. (2016). Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts. ONCOTARGET, 7(46), 75603–75615. https://doi.org/10.18632/oncotarget.12312
- Chicago author-date
- Tommelein, Joke, Félix Gremonprez, Laurine Verset, Elly De Vlieghere, Glenn Wagemans, Christian Gespach, Tom Boterberg, et al. 2016. “Age and Cellular Context Influence Rectal Prolapse Formation in Mice with Caecal Wall Colorectal Cancer Xenografts.” ONCOTARGET 7 (46): 75603–15. https://doi.org/10.18632/oncotarget.12312.
- Chicago author-date (all authors)
- Tommelein, Joke, Félix Gremonprez, Laurine Verset, Elly De Vlieghere, Glenn Wagemans, Christian Gespach, Tom Boterberg, Pieter Demetter, Wim Ceelen, marc bracke, and Olivier De Wever. 2016. “Age and Cellular Context Influence Rectal Prolapse Formation in Mice with Caecal Wall Colorectal Cancer Xenografts.” ONCOTARGET 7 (46): 75603–75615. doi:10.18632/oncotarget.12312.
- Vancouver
- 1.Tommelein J, Gremonprez F, Verset L, De Vlieghere E, Wagemans G, Gespach C, et al. Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts. ONCOTARGET. 2016;7(46):75603–15.
- IEEE
- [1]J. Tommelein et al., “Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts,” ONCOTARGET, vol. 7, no. 46, pp. 75603–75615, 2016.
@article{8502786,
abstract = {{In patients with rectal prolapse is the prevalence of colorectal cancer increased, suggesting that a colorectal tumor may induce rectal prolapse. Establishment of tumor xenografts in immunodeficient mice after orthotopic inoculations of human colorectal cancer cells into the caecal wall is a widely used approach for the study of human colorectal cancer progression and preclinical evaluation of therapeutics. Remarkably, 70% of young mice carrying a COLO320DM caecal tumor showed symptoms of intussusception of the large bowel associated with intestinal lumen obstruction and rectal prolapse. The quantity of the COLO320DM bioluminescent signal of the first three weeks post-inoculation predicts prolapse in young mice. Rectal prolapse was not observed in adult mice carrying a COLO320DM caecal tumor or young mice carrying a HT29 caecal tumor. In contrast to HT29 tumors, which showed local invasion and metastasis, COLO320DM tumors demonstrated a non-invasive tumor with pushing borders without presence of metastasis. In conclusion, rectal prolapse can be linked to a non-invasive, space-occupying COLO320DM tumor in the gastrointestinal tract of young immunodeficient mice. These data reveal a model that can clarify the association of patients showing rectal prolapse with colorectal cancer.}},
author = {{Tommelein, Joke and Gremonprez, Félix and Verset, Laurine and De Vlieghere, Elly and Wagemans, Glenn and Gespach, Christian and Boterberg, Tom and Demetter, Pieter and Ceelen, Wim and bracke, marc and De Wever, Olivier}},
issn = {{1949-2553}},
journal = {{ONCOTARGET}},
keywords = {{rectal prolapse,COLO320DM,colorectal cancer,orthotopic,mouse model,HUMAN-COLON-CARCINOMA,GROWTH-FACTOR RECEPTOR,NUDE-MICE,ENDOTHELIAL-CELLS,ULCER SYNDROME,IN-VIVO,METASTASIS,INTUSSUSCEPTION,INHIBITION,THERAPY}},
language = {{eng}},
number = {{46}},
pages = {{75603--75615}},
title = {{Age and cellular context influence rectal prolapse formation in mice with caecal wall colorectal cancer xenografts}},
url = {{http://doi.org/10.18632/oncotarget.12312}},
volume = {{7}},
year = {{2016}},
}
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