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Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model

Lore Van Hecke, Maarten Haspeslagh UGent, Katleen Hermans UGent and Ann Martens UGent (2016) AMERICAN JOURNAL OF VETERINARY RESEARCH. 77(12). p.1325-1331
abstract
OBJECTIVE : To compare antibacterial effects among 3 types of foam used with negative-pressure wound therapy (NPWT) in an ex vivo equine perfused wound model. SAMPLES : Abdominal musculocutaneous flaps from 6 equine cadavers. PROCEDURES : Each musculocutaneous flap was continuously perfused with saline (0.9% NaCl) solution. Four 5-cm circular wounds were created in each flap and contaminated with 10(6) CFUs of both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). After a 1-hour incubation period, 1 of 4 treatments (NPWT with silver-impregnated polyurethane foam [NPWT-AgPU], polyurethane foam [NPWT-PU], or polyvinyl alcohol foam [NPWT-PVA] or a nonadherent dressing containing polyhexamethylene biguanide without NPWT [control]) was randomly applied to each wound. An 8-mm punch biopsy specimen was obtained from each wound immediately before and at 6, 12, 18, and 24 hours after treatment application to determine the bacterial load for both P aeruginosa and MRSA. RESULTS : The bacterial load of P aeruginosa for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application, whereas the bacterial load for the NPWT-AgPU treatment was significantly lower than that for the NPWT-PU and control treatments at 12 hours after application. The bacterial load of MRSA for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application. CONCLUSIONS AND CLINICAL RELEVANCE : Results indicated that wounds treated with NPWT-PVA had the greatest decrease in bacterial load; however, the effect of that treatment on wound healing needs to be assessed in vivo.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
VACUUM-ASSISTED CLOSURE, EVIDENCE-BASED RECOMMENDATIONS, INTERNATIONAL CONSENSUS, MANAGEMENT, HORSE, STEPS
journal title
AMERICAN JOURNAL OF VETERINARY RESEARCH
Am. J. Vet. Res.
volume
77
issue
12
pages
1325 - 1331
Web of Science type
Article
Web of Science id
000389412100003
JCR category
VETERINARY SCIENCES
JCR impact factor
1.004 (2016)
JCR rank
59/136 (2016)
JCR quartile
2 (2016)
ISSN
0002-9645
1943-5681
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8502165
handle
http://hdl.handle.net/1854/LU-8502165
date created
2017-01-16 09:34:39
date last changed
2017-06-08 11:38:52
@article{8502165,
  abstract     = {OBJECTIVE : To compare antibacterial effects among 3 types of foam used with negative-pressure wound therapy (NPWT) in an ex vivo equine perfused wound model. 
SAMPLES : Abdominal musculocutaneous flaps from 6 equine cadavers. 
PROCEDURES : Each musculocutaneous flap was continuously perfused with saline (0.9\% NaCl) solution. Four 5-cm circular wounds were created in each flap and contaminated with 10(6) CFUs of both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). After a 1-hour incubation period, 1 of 4 treatments (NPWT with silver-impregnated polyurethane foam [NPWT-AgPU], polyurethane foam [NPWT-PU], or polyvinyl alcohol foam [NPWT-PVA] or a nonadherent dressing containing polyhexamethylene biguanide without NPWT [control]) was randomly applied to each wound. An 8-mm punch biopsy specimen was obtained from each wound immediately before and at 6, 12, 18, and 24 hours after treatment application to determine the bacterial load for both P aeruginosa and MRSA. 
RESULTS : The bacterial load of P aeruginosa for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application, whereas the bacterial load for the NPWT-AgPU treatment was significantly lower than that for the NPWT-PU and control treatments at 12 hours after application. The bacterial load of MRSA for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application. 
CONCLUSIONS AND CLINICAL RELEVANCE : Results indicated that wounds treated with NPWT-PVA had the greatest decrease in bacterial load; however, the effect of that treatment on wound healing needs to be assessed in vivo.},
  author       = {Van Hecke, Lore and Haspeslagh, Maarten and Hermans, Katleen and Martens, Ann},
  issn         = {0002-9645},
  journal      = {AMERICAN JOURNAL OF VETERINARY RESEARCH},
  keyword      = {VACUUM-ASSISTED CLOSURE,EVIDENCE-BASED RECOMMENDATIONS,INTERNATIONAL CONSENSUS,MANAGEMENT,HORSE,STEPS},
  language     = {eng},
  number       = {12},
  pages        = {1325--1331},
  title        = {Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model},
  volume       = {77},
  year         = {2016},
}

Chicago
Van Hecke, Lore, Maarten Haspeslagh, Katleen Hermans, and Ann Martens. 2016. “Comparison of Antibacterial Effects Among Three Foams Used with Negative Pressure Wound Therapy in an Ex Vivo Equine Perfused Wound Model.” American Journal of Veterinary Research 77 (12): 1325–1331.
APA
Van Hecke, Lore, Haspeslagh, M., Hermans, K., & Martens, A. (2016). Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model. AMERICAN JOURNAL OF VETERINARY RESEARCH, 77(12), 1325–1331.
Vancouver
1.
Van Hecke L, Haspeslagh M, Hermans K, Martens A. Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model. AMERICAN JOURNAL OF VETERINARY RESEARCH. 2016;77(12):1325–31.
MLA
Van Hecke, Lore, Maarten Haspeslagh, Katleen Hermans, et al. “Comparison of Antibacterial Effects Among Three Foams Used with Negative Pressure Wound Therapy in an Ex Vivo Equine Perfused Wound Model.” AMERICAN JOURNAL OF VETERINARY RESEARCH 77.12 (2016): 1325–1331. Print.