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Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model

Lore Van Hecke (UGent) , Maarten Haspeslagh (UGent) , Katleen Hermans (UGent) and Ann Martens (UGent)
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Abstract
OBJECTIVE : To compare antibacterial effects among 3 types of foam used with negative-pressure wound therapy (NPWT) in an ex vivo equine perfused wound model. SAMPLES : Abdominal musculocutaneous flaps from 6 equine cadavers. PROCEDURES : Each musculocutaneous flap was continuously perfused with saline (0.9% NaCl) solution. Four 5-cm circular wounds were created in each flap and contaminated with 10(6) CFUs of both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). After a 1-hour incubation period, 1 of 4 treatments (NPWT with silver-impregnated polyurethane foam [NPWT-AgPU], polyurethane foam [NPWT-PU], or polyvinyl alcohol foam [NPWT-PVA] or a nonadherent dressing containing polyhexamethylene biguanide without NPWT [control]) was randomly applied to each wound. An 8-mm punch biopsy specimen was obtained from each wound immediately before and at 6, 12, 18, and 24 hours after treatment application to determine the bacterial load for both P aeruginosa and MRSA. RESULTS : The bacterial load of P aeruginosa for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application, whereas the bacterial load for the NPWT-AgPU treatment was significantly lower than that for the NPWT-PU and control treatments at 12 hours after application. The bacterial load of MRSA for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application. CONCLUSIONS AND CLINICAL RELEVANCE : Results indicated that wounds treated with NPWT-PVA had the greatest decrease in bacterial load; however, the effect of that treatment on wound healing needs to be assessed in vivo.
Keywords
VACUUM-ASSISTED CLOSURE, EVIDENCE-BASED RECOMMENDATIONS, INTERNATIONAL CONSENSUS, MANAGEMENT, HORSE, STEPS

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Chicago
Van Hecke, Lore, Maarten Haspeslagh, Katleen Hermans, and Ann Martens. 2016. “Comparison of Antibacterial Effects Among Three Foams Used with Negative Pressure Wound Therapy in an Ex Vivo Equine Perfused Wound Model.” American Journal of Veterinary Research 77 (12): 1325–1331.
APA
Van Hecke, Lore, Haspeslagh, M., Hermans, K., & Martens, A. (2016). Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model. AMERICAN JOURNAL OF VETERINARY RESEARCH, 77(12), 1325–1331.
Vancouver
1.
Van Hecke L, Haspeslagh M, Hermans K, Martens A. Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model. AMERICAN JOURNAL OF VETERINARY RESEARCH. 2016;77(12):1325–31.
MLA
Van Hecke, Lore, Maarten Haspeslagh, Katleen Hermans, et al. “Comparison of Antibacterial Effects Among Three Foams Used with Negative Pressure Wound Therapy in an Ex Vivo Equine Perfused Wound Model.” AMERICAN JOURNAL OF VETERINARY RESEARCH 77.12 (2016): 1325–1331. Print.
@article{8502165,
  abstract     = {OBJECTIVE : To compare antibacterial effects among 3 types of foam used with negative-pressure wound therapy (NPWT) in an ex vivo equine perfused wound model. 
SAMPLES : Abdominal musculocutaneous flaps from 6 equine cadavers. 
PROCEDURES : Each musculocutaneous flap was continuously perfused with saline (0.9\% NaCl) solution. Four 5-cm circular wounds were created in each flap and contaminated with 10(6) CFUs of both Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA). After a 1-hour incubation period, 1 of 4 treatments (NPWT with silver-impregnated polyurethane foam [NPWT-AgPU], polyurethane foam [NPWT-PU], or polyvinyl alcohol foam [NPWT-PVA] or a nonadherent dressing containing polyhexamethylene biguanide without NPWT [control]) was randomly applied to each wound. An 8-mm punch biopsy specimen was obtained from each wound immediately before and at 6, 12, 18, and 24 hours after treatment application to determine the bacterial load for both P aeruginosa and MRSA. 
RESULTS : The bacterial load of P aeruginosa for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application, whereas the bacterial load for the NPWT-AgPU treatment was significantly lower than that for the NPWT-PU and control treatments at 12 hours after application. The bacterial load of MRSA for the NPWT-PVA treatment was significantly lower than that for the other 3 treatments at each sampling time after application. 
CONCLUSIONS AND CLINICAL RELEVANCE : Results indicated that wounds treated with NPWT-PVA had the greatest decrease in bacterial load; however, the effect of that treatment on wound healing needs to be assessed in vivo.},
  author       = {Van Hecke, Lore and Haspeslagh, Maarten and Hermans, Katleen and Martens, Ann},
  issn         = {0002-9645},
  journal      = {AMERICAN JOURNAL OF VETERINARY RESEARCH},
  keyword      = {VACUUM-ASSISTED CLOSURE,EVIDENCE-BASED RECOMMENDATIONS,INTERNATIONAL CONSENSUS,MANAGEMENT,HORSE,STEPS},
  language     = {eng},
  number       = {12},
  pages        = {1325--1331},
  title        = {Comparison of antibacterial effects among three foams used with negative pressure wound therapy in an ex vivo equine perfused wound model},
  volume       = {77},
  year         = {2016},
}

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