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Analysis of polymorphic membrane protein expression in cultured cells Identifies PmpA and PmpH of Chlamydia psittaci as candidate factors in pathogenesis and immunity to infection

(2016) PLOS ONE. 11(9).
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Abstract
The polymorphic membrane protein (Pmp) paralogous families of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia abortus are putative targets for Chlamydia vaccine development. To determine whether this is also the case for Pmp family members of C. psittaci, we analyzed transcription levels, protein production and localization of several Pmps of C. psittaci. Pmp expression profiles were characterized using quantitative real-time PCR (RT-qPCR), immunofluorescence (IF) and immuno-electron microscopy (IEM) under normal and stress conditions. We found that PmpA was highly produced in all inclusions as early as 12 hpi in all biological replicates. In addition, PmpA and PmpH appeared to be unusually accessible to antibody as determined by both immunofluorescence and immuno-electron microscopy. Our results suggest an important role for these Pmps in the pathogenesis of C. psittaci, and make them promising candidates in vaccine development.
Keywords
CHLAMYDOPHILA-PSITTACI, DEVELOPMENTAL CYCLE, ANTIGENIC VARIATION, GENOME, SEQUENCES, TRACHOMATIS, FAMILY, PNEUMONIAE, SURFACE, ABORTION, STRAINS

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MLA
Van Lent, Sarah et al. “Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia Psittaci as Candidate Factors in Pathogenesis and Immunity to Infection.” PLOS ONE 11.9 (2016): n. pag. Print.
APA
Van Lent, S., De Vos, W., Creasy, H. H., Marques, P. X., Ravel, J., Vanrompay, D., Bavoil, P., et al. (2016). Analysis of polymorphic membrane protein expression in cultured cells Identifies PmpA and PmpH of Chlamydia psittaci as candidate factors in pathogenesis and immunity to infection. PLOS ONE, 11(9).
Chicago author-date
Van Lent, Sarah, Winnok De Vos, Heather Huot Creasy, Patricia X Marques, Jacques Ravel, Daisy Vanrompay, Patrik Bavoil, and Ru-ching Hsia. 2016. “Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia Psittaci as Candidate Factors in Pathogenesis and Immunity to Infection.” Plos One 11 (9).
Chicago author-date (all authors)
Van Lent, Sarah, Winnok De Vos, Heather Huot Creasy, Patricia X Marques, Jacques Ravel, Daisy Vanrompay, Patrik Bavoil, and Ru-ching Hsia. 2016. “Analysis of Polymorphic Membrane Protein Expression in Cultured Cells Identifies PmpA and PmpH of Chlamydia Psittaci as Candidate Factors in Pathogenesis and Immunity to Infection.” Plos One 11 (9).
Vancouver
1.
Van Lent S, De Vos W, Creasy HH, Marques PX, Ravel J, Vanrompay D, et al. Analysis of polymorphic membrane protein expression in cultured cells Identifies PmpA and PmpH of Chlamydia psittaci as candidate factors in pathogenesis and immunity to infection. PLOS ONE. 2016;11(9).
IEEE
[1]
S. Van Lent et al., “Analysis of polymorphic membrane protein expression in cultured cells Identifies PmpA and PmpH of Chlamydia psittaci as candidate factors in pathogenesis and immunity to infection,” PLOS ONE, vol. 11, no. 9, 2016.
@article{8502018,
  abstract     = {The polymorphic membrane protein (Pmp) paralogous families of Chlamydia trachomatis, Chlamydia pneumoniae and Chlamydia abortus are putative targets for Chlamydia vaccine development. To determine whether this is also the case for Pmp family members of C. psittaci, we analyzed transcription levels, protein production and localization of several Pmps of C. psittaci. Pmp expression profiles were characterized using quantitative real-time PCR (RT-qPCR), immunofluorescence (IF) and immuno-electron microscopy (IEM) under normal and stress conditions. We found that PmpA was highly produced in all inclusions as early as 12 hpi in all biological replicates. In addition, PmpA and PmpH appeared to be unusually accessible to antibody as determined by both immunofluorescence and immuno-electron microscopy. Our results suggest an important role for these Pmps in the pathogenesis of C. psittaci, and make them promising candidates in vaccine development.},
  articleno    = {e0162392},
  author       = {Van Lent, Sarah and De Vos, Winnok and Creasy, Heather Huot and Marques, Patricia X and Ravel, Jacques and Vanrompay, Daisy and Bavoil, Patrik and Hsia, Ru-ching},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {CHLAMYDOPHILA-PSITTACI,DEVELOPMENTAL CYCLE,ANTIGENIC VARIATION,GENOME,SEQUENCES,TRACHOMATIS,FAMILY,PNEUMONIAE,SURFACE,ABORTION,STRAINS},
  language     = {eng},
  number       = {9},
  pages        = {27},
  title        = {Analysis of polymorphic membrane protein expression in cultured cells Identifies PmpA and PmpH of Chlamydia psittaci as candidate factors in pathogenesis and immunity to infection},
  url          = {http://dx.doi.org/10.1371/journal.pone.0162392},
  volume       = {11},
  year         = {2016},
}

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