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Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity

Tom Verhaeghe, Karel De Winter, Magali Berland, Rob De Vreese, Matthias D'hooghe UGent, Bernard Offmann and Tom Desmet UGent (2016) CHEMICAL COMMUNICATIONS. 52(18). p.3687-3689
abstract
Despite the growing importance of prebiotics in nutrition and gastroenterology, their structural variety is currently still very limited. The lack of straightforward procedures to gain new products in sufficient amounts often hampers application testing and further development. Although the enzyme sucrose phosphorylase can be used to produce the rare disaccharide kojibiose (alpha-1,2-glucobiose) from the bulk sugars sucrose and glucose, the target compound is only a side product that is difficult to isolate. Accordingly, for this biocatalyst to become economically attractive, the formation of other glucobioses should be avoided and therefore we applied semi-rational mutagenesis and low-throughput screening, which resulted in a double mutant (L341I_Q345S) with a selectivity of 95% for kojibiose. That way, an efficient and scalable production process with a yield of 74% could be established, andwith a simple yeast treatment and crystallization step over a hundred grams of highly pure kojibiose (>99.5%) was obtained.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
SUCROSE PHOSPHORYLASE, BIFIDOBACTERIUM-ADOLESCENTIS, KOJIBIOSE, GLUCOAMYLASE, MICROBIOME
journal title
CHEMICAL COMMUNICATIONS
Chem. Commun.
volume
52
issue
18
pages
3687 - 3689
Web of Science type
Article
Web of Science id
000371008500022
JCR category
CHEMISTRY, MULTIDISCIPLINARY
JCR impact factor
6.319 (2016)
JCR rank
23/166 (2016)
JCR quartile
1 (2016)
ISSN
1359-7345
1364-548X
DOI
10.1039/c5cc09940d
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8500768
handle
http://hdl.handle.net/1854/LU-8500768
date created
2017-01-09 11:51:11
date last changed
2017-03-13 12:52:39
@article{8500768,
  abstract     = {Despite the growing importance of prebiotics in nutrition and gastroenterology, their structural variety is currently still very limited. The lack of straightforward procedures to gain new products in sufficient amounts often hampers application testing and further development. Although the enzyme sucrose phosphorylase can be used to produce the rare disaccharide kojibiose (alpha-1,2-glucobiose) from the bulk sugars sucrose and glucose, the target compound is only a side product that is difficult to isolate. Accordingly, for this biocatalyst to become economically attractive, the formation of other glucobioses should be avoided and therefore we applied semi-rational mutagenesis and low-throughput screening, which resulted in a double mutant (L341I\_Q345S) with a selectivity of 95\% for kojibiose. That way, an efficient and scalable production process with a yield of 74\% could be established, andwith a simple yeast treatment and crystallization step over a hundred grams of highly pure kojibiose ({\textrangle}99.5\%) was obtained.},
  author       = {Verhaeghe, Tom and De Winter, Karel and Berland, Magali and De Vreese, Rob and D'hooghe, Matthias and Offmann, Bernard and Desmet, Tom},
  issn         = {1359-7345},
  journal      = {CHEMICAL COMMUNICATIONS},
  keyword      = {SUCROSE PHOSPHORYLASE,BIFIDOBACTERIUM-ADOLESCENTIS,KOJIBIOSE,GLUCOAMYLASE,MICROBIOME},
  language     = {eng},
  number       = {18},
  pages        = {3687--3689},
  title        = {Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity},
  url          = {http://dx.doi.org/10.1039/c5cc09940d},
  volume       = {52},
  year         = {2016},
}

Chicago
Verhaeghe, Tom, Karel De Winter, Magali Berland, Rob De Vreese, Matthias D’hooghe, Bernard Offmann, and Tom Desmet. 2016. “Converting Bulk Sugars into Prebiotics : Semirational Design of a Transglucosylase with Controlled Selectivity.” Chemical Communications 52 (18): 3687–3689.
APA
Verhaeghe, T., De Winter, K., Berland, M., De Vreese, R., D’hooghe, M., Offmann, B., & Desmet, T. (2016). Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity. CHEMICAL COMMUNICATIONS, 52(18), 3687–3689.
Vancouver
1.
Verhaeghe T, De Winter K, Berland M, De Vreese R, D’hooghe M, Offmann B, et al. Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity. CHEMICAL COMMUNICATIONS. 2016;52(18):3687–9.
MLA
Verhaeghe, Tom, Karel De Winter, Magali Berland, et al. “Converting Bulk Sugars into Prebiotics : Semirational Design of a Transglucosylase with Controlled Selectivity.” CHEMICAL COMMUNICATIONS 52.18 (2016): 3687–3689. Print.