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Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity

(2016) CHEMICAL COMMUNICATIONS. 52(18). p.3687-3689
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Abstract
Despite the growing importance of prebiotics in nutrition and gastroenterology, their structural variety is currently still very limited. The lack of straightforward procedures to gain new products in sufficient amounts often hampers application testing and further development. Although the enzyme sucrose phosphorylase can be used to produce the rare disaccharide kojibiose (alpha-1,2-glucobiose) from the bulk sugars sucrose and glucose, the target compound is only a side product that is difficult to isolate. Accordingly, for this biocatalyst to become economically attractive, the formation of other glucobioses should be avoided and therefore we applied semi-rational mutagenesis and low-throughput screening, which resulted in a double mutant (L341I_Q345S) with a selectivity of 95% for kojibiose. That way, an efficient and scalable production process with a yield of 74% could be established, andwith a simple yeast treatment and crystallization step over a hundred grams of highly pure kojibiose (>99.5%) was obtained.
Keywords
SUCROSE PHOSPHORYLASE, BIFIDOBACTERIUM-ADOLESCENTIS, KOJIBIOSE, GLUCOAMYLASE, MICROBIOME

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Chicago
Verhaeghe, Tom, Karel De Winter, Magali Berland, Rob De Vreese, Matthias D’hooghe, Bernard Offmann, and Tom Desmet. 2016. “Converting Bulk Sugars into Prebiotics : Semirational Design of a Transglucosylase with Controlled Selectivity.” Chemical Communications 52 (18): 3687–3689.
APA
Verhaeghe, T., De Winter, K., Berland, M., De Vreese, R., D’hooghe, M., Offmann, B., & Desmet, T. (2016). Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity. CHEMICAL COMMUNICATIONS, 52(18), 3687–3689.
Vancouver
1.
Verhaeghe T, De Winter K, Berland M, De Vreese R, D’hooghe M, Offmann B, et al. Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity. CHEMICAL COMMUNICATIONS. 2016;52(18):3687–9.
MLA
Verhaeghe, Tom, Karel De Winter, Magali Berland, et al. “Converting Bulk Sugars into Prebiotics : Semirational Design of a Transglucosylase with Controlled Selectivity.” CHEMICAL COMMUNICATIONS 52.18 (2016): 3687–3689. Print.
@article{8500768,
  abstract     = {Despite the growing importance of prebiotics in nutrition and gastroenterology, their structural variety is currently still very limited. The lack of straightforward procedures to gain new products in sufficient amounts often hampers application testing and further development. Although the enzyme sucrose phosphorylase can be used to produce the rare disaccharide kojibiose (alpha-1,2-glucobiose) from the bulk sugars sucrose and glucose, the target compound is only a side product that is difficult to isolate. Accordingly, for this biocatalyst to become economically attractive, the formation of other glucobioses should be avoided and therefore we applied semi-rational mutagenesis and low-throughput screening, which resulted in a double mutant (L341I\_Q345S) with a selectivity of 95\% for kojibiose. That way, an efficient and scalable production process with a yield of 74\% could be established, andwith a simple yeast treatment and crystallization step over a hundred grams of highly pure kojibiose ({\textrangle}99.5\%) was obtained.},
  author       = {Verhaeghe, Tom and De Winter, Karel and Berland, Magali and De Vreese, Rob and D'hooghe, Matthias and Offmann, Bernard and Desmet, Tom},
  issn         = {1359-7345},
  journal      = {CHEMICAL COMMUNICATIONS},
  keyword      = {SUCROSE PHOSPHORYLASE,BIFIDOBACTERIUM-ADOLESCENTIS,KOJIBIOSE,GLUCOAMYLASE,MICROBIOME},
  language     = {eng},
  number       = {18},
  pages        = {3687--3689},
  title        = {Converting bulk sugars into prebiotics : semirational design of a transglucosylase with controlled selectivity},
  url          = {http://dx.doi.org/10.1039/c5cc09940d},
  volume       = {52},
  year         = {2016},
}

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