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A multilevel framework to reconstruct anatomical 3D models of the hepatic vasculature in rat livers

Geert Peeters UGent, Charlotte Debbaut UGent, Wim Laleman, Diethard Monbaliu, Ingrid Vander Elst, Jan R. Detrez, Tim Vandecasteele UGent, Thomas De Schryver UGent, Luc Van Hoorebeke UGent, Kasper Favere, et al. (2017) JOURNAL OF ANATOMY. 230(3). p.471-483
abstract
The intricate (micro) vascular architecture of the liver has not yet been fully unravelled. Although current models are often idealized simplifications of the complex anatomical reality, correct morphological information is instrumental for scientific and clinical purposes. Previously, both vascular corrosion casting (VCC) and immunohistochemistry (IHC) have been separately used to study the hepatic vasculature. Nevertheless, these techniques still face a number of challenges such as dual casting in VCC and limited imaging depths for IHC. We have optimized both techniques and combined their complementary strengths to develop a framework for multilevel reconstruction of the hepatic circulation in the rat. The VCC and micro-CT scanning protocol was improved by enabling dual casting, optimizing the contrast agent concentration, and adjusting the viscosity of the resin (PU4ii). IHC was improved with an optimized clearing technique (CUBIC) that extended the imaging depth for confocal microscopy more than five-fold. Using in-house developed software (DeLiver), the vascular network - in both VCC and IHC datasets - was automatically segmented and/or morphologically analysed. Our methodological framework allows 3D reconstruction and quantification of the hepatic circulation, ranging from the major blood vessels down to the intertwined and interconnected sinusoids. We believe that the presented framework will have value beyond studies of the liver, and will facilitate a better understanding of various parenchymal organs in general, in physiological and pathological circumstances. Key words: 3D modeling; confocal laser scanning; hepatic vasculature; immunohistochemistry; micro-CTscanning; morphological analysis; rat liver; vascular corrosion casting.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Anatomy, Developmental Biology, Cell Biology, Ecology, Evolution, Behavior and Systematics, Molecular Biology, Histology, WHOLE-BRAIN, BLOOD-FLOW, TISSUE, MICROSCOPY, ARCHITECTURE, MICROCIRCULATION, QUANTIFICATION, HEMODYNAMICS, SINUSOIDS, PROTOCOLS
journal title
JOURNAL OF ANATOMY
J. Anat.
volume
230
issue
3
pages
471 - 483
Web of Science type
Article
Web of Science id
000394894900011
ISSN
0021-8782
DOI
10.1111/joa.12567
language
English
UGent publication?
yes
classification
A1
additional info
the last two authors share senior co-authorship
copyright statement
I have transferred the copyright for this publication to the publisher
id
8500684
handle
http://hdl.handle.net/1854/LU-8500684
date created
2017-01-09 09:56:03
date last changed
2017-09-14 09:22:13
@article{8500684,
  abstract     = {The intricate (micro) vascular architecture of the liver has not yet been fully unravelled. Although current models are often idealized simplifications of the complex anatomical reality, correct morphological information is instrumental for scientific and clinical purposes. Previously, both vascular corrosion casting (VCC) and immunohistochemistry (IHC) have been separately used to study the hepatic vasculature. Nevertheless, these techniques still face a number of challenges such as dual casting in VCC and limited imaging depths for IHC. We have optimized both techniques and combined their complementary strengths to develop a framework for multilevel reconstruction of the hepatic circulation in the rat. The VCC and micro-CT scanning protocol was improved by enabling dual casting, optimizing the contrast agent concentration, and adjusting the viscosity of the resin (PU4ii). IHC was improved with an optimized clearing technique (CUBIC) that extended the imaging depth for confocal microscopy more than five-fold. Using in-house developed software (DeLiver), the vascular network - in both VCC and IHC datasets - was automatically segmented and/or morphologically analysed. Our methodological framework allows 3D reconstruction and quantification of the hepatic circulation, ranging from the major blood vessels down to the intertwined and interconnected sinusoids. We believe that the presented framework will have value beyond studies of the liver, and will facilitate a better understanding of various parenchymal organs in general, in physiological and pathological circumstances. Key words: 3D modeling; confocal laser scanning; hepatic vasculature; immunohistochemistry; micro-CTscanning; morphological analysis; rat liver; vascular corrosion casting.},
  author       = {Peeters, Geert and Debbaut, Charlotte and Laleman, Wim and Monbaliu, Diethard and Vander Elst, Ingrid and Detrez, Jan R. and Vandecasteele, Tim and De Schryver, Thomas and Van Hoorebeke, Luc and Favere, Kasper and Verbeke, Jonas and Segers, Patrick and Cornillie, Pieter and De Vos, Winnok},
  issn         = {0021-8782},
  journal      = {JOURNAL OF ANATOMY},
  keyword      = {Anatomy,Developmental Biology,Cell Biology,Ecology,Evolution,Behavior and Systematics,Molecular Biology,Histology,WHOLE-BRAIN,BLOOD-FLOW,TISSUE,MICROSCOPY,ARCHITECTURE,MICROCIRCULATION,QUANTIFICATION,HEMODYNAMICS,SINUSOIDS,PROTOCOLS},
  language     = {eng},
  number       = {3},
  pages        = {471--483},
  title        = {A multilevel framework to reconstruct anatomical 3D models of the hepatic vasculature in rat livers},
  url          = {http://dx.doi.org/10.1111/joa.12567},
  volume       = {230},
  year         = {2017},
}

Chicago
Peeters, Geert, Charlotte Debbaut, Wim Laleman, Diethard Monbaliu, Ingrid Vander Elst, Jan R. Detrez, Tim Vandecasteele, et al. 2017. “A Multilevel Framework to Reconstruct Anatomical 3D Models of the Hepatic Vasculature in Rat Livers.” Journal of Anatomy 230 (3): 471–483.
APA
Peeters, Geert, Debbaut, C., Laleman, W., Monbaliu, D., Vander Elst, I., Detrez, J. R., Vandecasteele, T., et al. (2017). A multilevel framework to reconstruct anatomical 3D models of the hepatic vasculature in rat livers. JOURNAL OF ANATOMY, 230(3), 471–483.
Vancouver
1.
Peeters G, Debbaut C, Laleman W, Monbaliu D, Vander Elst I, Detrez JR, et al. A multilevel framework to reconstruct anatomical 3D models of the hepatic vasculature in rat livers. JOURNAL OF ANATOMY. 2017;230(3):471–83.
MLA
Peeters, Geert, Charlotte Debbaut, Wim Laleman, et al. “A Multilevel Framework to Reconstruct Anatomical 3D Models of the Hepatic Vasculature in Rat Livers.” JOURNAL OF ANATOMY 230.3 (2017): 471–483. Print.