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Enquiry into the topology of plasma membrane-localized PIN auxin transport components

Tomasz Nodzyński, Steffen Vanneste UGent, Marta Zwiewka, Markéta Pernisová, Jan Hejátko and Jiri Friml (2016) MOLECULAR PLANT. 9(11). p.1504-1519
abstract
Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques, we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five alpha-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1. Since the canonical long PINs show a high degree of conservation in TM domains and auxin transport capacity has been demonstrated for Arabidopsis representatives of this group, this empirically enhanced topological model of PIN1 will be an important starting point for further studies on PIN structure-function relationships. In addition, we have established protocols that can be used to probe the topology of other plasma membrane proteins in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
plasma membrane protein, topology, auxin efflux carriers, Arabidopsis thaliana, PROTEIN TOPOLOGY, PLANT DEVELOPMENT, INTRACELLULAR TRAFFICKING, ROOT GRAVITROPISM, COATED VESICLES, ARABIDOPSIS, EFFLUX, PREDICTION, PHOSPHORYLATION, FIXATION
journal title
MOLECULAR PLANT
Mol. Plant.
volume
9
issue
11
pages
1504 - 1519
Web of Science type
Article
Web of Science id
000389594100008
JCR category
PLANT SCIENCES
JCR impact factor
8.827 (2016)
JCR rank
5/211 (2016)
JCR quartile
1 (2016)
ISSN
1674-2052
1752-9867
DOI
10.1016/j.molp.2016.08.010
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8500660
handle
http://hdl.handle.net/1854/LU-8500660
date created
2017-01-09 09:26:27
date last changed
2017-01-20 15:33:12
@article{8500660,
  abstract     = {Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques, we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five alpha-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1. Since the canonical long PINs show a high degree of conservation in TM domains and auxin transport capacity has been demonstrated for Arabidopsis representatives of this group, this empirically enhanced topological model of PIN1 will be an important starting point for further studies on PIN structure-function relationships. In addition, we have established protocols that can be used to probe the topology of other plasma membrane proteins in plants.},
  author       = {Nodzy\'{n}ski, Tomasz and Vanneste, Steffen and Zwiewka, Marta and Pernisov{\'a}, Mark{\'e}ta and Hej{\'a}tko, Jan and Friml, Jiri},
  issn         = {1674-2052},
  journal      = {MOLECULAR PLANT},
  keyword      = {plasma membrane protein,topology,auxin efflux carriers,Arabidopsis thaliana,PROTEIN TOPOLOGY,PLANT DEVELOPMENT,INTRACELLULAR TRAFFICKING,ROOT GRAVITROPISM,COATED VESICLES,ARABIDOPSIS,EFFLUX,PREDICTION,PHOSPHORYLATION,FIXATION},
  language     = {eng},
  number       = {11},
  pages        = {1504--1519},
  title        = {Enquiry into the topology of plasma membrane-localized PIN auxin transport components},
  url          = {http://dx.doi.org/10.1016/j.molp.2016.08.010},
  volume       = {9},
  year         = {2016},
}

Chicago
Nodzyński, Tomasz, Steffen Vanneste, Marta Zwiewka, Markéta Pernisová, Jan Hejátko, and Jiri Friml. 2016. “Enquiry into the Topology of Plasma Membrane-localized PIN Auxin Transport Components.” Molecular Plant 9 (11): 1504–1519.
APA
Nodzyński, T., Vanneste, S., Zwiewka, M., Pernisová, M., Hejátko, J., & Friml, J. (2016). Enquiry into the topology of plasma membrane-localized PIN auxin transport components. MOLECULAR PLANT, 9(11), 1504–1519.
Vancouver
1.
Nodzyński T, Vanneste S, Zwiewka M, Pernisová M, Hejátko J, Friml J. Enquiry into the topology of plasma membrane-localized PIN auxin transport components. MOLECULAR PLANT. 2016;9(11):1504–19.
MLA
Nodzyński, Tomasz, Steffen Vanneste, Marta Zwiewka, et al. “Enquiry into the Topology of Plasma Membrane-localized PIN Auxin Transport Components.” MOLECULAR PLANT 9.11 (2016): 1504–1519. Print.