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Transcript profiling for early stages during embryo development in Scots pine

Irene Merino, Malin Abrahamsson, Lieven Sterck UGent, Blanca Craven-Bartle, Francisco Canovas and Sara von Arnold (2016) BMC PLANT BIOLOGY. 16.
abstract
Background: Characterization of the expression and function of genes regulating embryo development in conifers is interesting from an evolutionary point of view. However, our knowledge about the regulation of embryo development in conifers is limited. During early embryo development in Pinus species the proembyo goes through a cleavage process, named cleavage polyembryony, giving rise to four embryos. One of these embryos develops to a dominant embryo, which will develop further into a mature, cotyledonary embryo, while the other embryos, the subordinate embryos, are degraded. The main goal of this study has been to identify processes that might be important for regulating the cleavage process and for the development of a dominant embryo. Results: RNA samples from embryos and megagametophytes at four early developmental stages during seed development in Pinus sylvestris were subjected to high-throughput sequencing. A total of 6.6 million raw reads was generated, resulting in 121,938 transcripts, out of which 36.106 contained ORFs. 18,638 transcripts were differentially expressed (DETs) in embryos and megagametophytes. GO enrichment analysis of transcripts up-regulated in embryos showed enrichment for different cellular processes, while those up-regulated in megagametophytes were enriched for accumulation of storage material and responses to stress. The highest number of DETs was detected during the initiation of the cleavage process. Transcripts related to embryogenic competence, cell wall modifications, cell division pattern, axis specification and response to hormones and stress were highly abundant and differentially expressed during early embryo development. The abundance of representative DETs was confirmed by qRT-PCR analyses. Conclusion: Based on the processes identified in the GO enrichment analyses and the expression of the selected transcripts we suggest that (i) processes related to embryogenic competence and cell wall loosening are involved in activating the cleavage process; (ii) apical-basal polarization is strictly regulated in dominant embryos but not in the subordinate embryos; (iii) the transition from the morphogenic phase to the maturation phase is not completed in subordinate embryos. This is the first genome-wide transcript expression profiling of the earliest stages during embryo development in a Pinus species. Our results can serve as a framework for future studies to reveal the functions of identified genes.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Embryo development, Pinus sylvestris L, Polyembryony, RNA-seq, Transcriptomic analysis, Zygotic embryogenesis, PROGRAMMED CELL-DEATH, SPRUCE PICEA-ABIES, NORWAY SPRUCE, ARABIDOPSIS-THALIANA, SEED-GERMINATION, SEQUENCING DATA, EXPRESSION, AUXIN, GENE, EMBRYOGENESIS
journal title
BMC PLANT BIOLOGY
BMC Plant Biol.
volume
16
article number
255
pages
18 pages
Web of Science type
Article
Web of Science id
000389481700001
JCR category
PLANT SCIENCES
JCR impact factor
3.964 (2016)
JCR rank
23/211 (2016)
JCR quartile
1 (2016)
ISSN
1471-2229
DOI
10.1186/s12870-016-0939-5
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8500656
handle
http://hdl.handle.net/1854/LU-8500656
date created
2017-01-09 09:18:09
date last changed
2017-01-20 14:53:51
@article{8500656,
  abstract     = {Background: Characterization of the expression and function of genes regulating embryo development in conifers is interesting from an evolutionary point of view. However, our knowledge about the regulation of embryo development in conifers is limited. During early embryo development in Pinus species the proembyo goes through a cleavage process, named cleavage polyembryony, giving rise to four embryos. One of these embryos develops to a dominant embryo, which will develop further into a mature, cotyledonary embryo, while the other embryos, the subordinate embryos, are degraded. The main goal of this study has been to identify processes that might be important for regulating the cleavage process and for the development of a dominant embryo. 
Results: RNA samples from embryos and megagametophytes at four early developmental stages during seed development in Pinus sylvestris were subjected to high-throughput sequencing. A total of 6.6 million raw reads was generated, resulting in 121,938 transcripts, out of which 36.106 contained ORFs. 18,638 transcripts were differentially expressed (DETs) in embryos and megagametophytes. GO enrichment analysis of transcripts up-regulated in embryos showed enrichment for different cellular processes, while those up-regulated in megagametophytes were enriched for accumulation of storage material and responses to stress. The highest number of DETs was detected during the initiation of the cleavage process. Transcripts related to embryogenic competence, cell wall modifications, cell division pattern, axis specification and response to hormones and stress were highly abundant and differentially expressed during early embryo development. The abundance of representative DETs was confirmed by qRT-PCR analyses. 
Conclusion: Based on the processes identified in the GO enrichment analyses and the expression of the selected transcripts we suggest that (i) processes related to embryogenic competence and cell wall loosening are involved in activating the cleavage process; (ii) apical-basal polarization is strictly regulated in dominant embryos but not in the subordinate embryos; (iii) the transition from the morphogenic phase to the maturation phase is not completed in subordinate embryos. This is the first genome-wide transcript expression profiling of the earliest stages during embryo development in a Pinus species. Our results can serve as a framework for future studies to reveal the functions of identified genes.},
  articleno    = {255},
  author       = {Merino, Irene and Abrahamsson, Malin and Sterck, Lieven and Craven-Bartle, Blanca and Canovas, Francisco and von Arnold, Sara},
  issn         = {1471-2229},
  journal      = {BMC PLANT BIOLOGY},
  keyword      = {Embryo development,Pinus sylvestris L,Polyembryony,RNA-seq,Transcriptomic analysis,Zygotic embryogenesis,PROGRAMMED CELL-DEATH,SPRUCE PICEA-ABIES,NORWAY SPRUCE,ARABIDOPSIS-THALIANA,SEED-GERMINATION,SEQUENCING DATA,EXPRESSION,AUXIN,GENE,EMBRYOGENESIS},
  pages        = {18},
  title        = {Transcript profiling for early stages during embryo development in Scots pine},
  url          = {http://dx.doi.org/10.1186/s12870-016-0939-5},
  volume       = {16},
  year         = {2016},
}

Chicago
Merino, Irene, Malin Abrahamsson, Lieven Sterck, Blanca Craven-Bartle, Francisco Canovas, and Sara von Arnold. 2016. “Transcript Profiling for Early Stages During Embryo Development in Scots Pine.” Bmc Plant Biology 16.
APA
Merino, I., Abrahamsson, M., Sterck, L., Craven-Bartle, B., Canovas, F., & von Arnold, S. (2016). Transcript profiling for early stages during embryo development in Scots pine. BMC PLANT BIOLOGY, 16.
Vancouver
1.
Merino I, Abrahamsson M, Sterck L, Craven-Bartle B, Canovas F, von Arnold S. Transcript profiling for early stages during embryo development in Scots pine. BMC PLANT BIOLOGY. 2016;16.
MLA
Merino, Irene, Malin Abrahamsson, Lieven Sterck, et al. “Transcript Profiling for Early Stages During Embryo Development in Scots Pine.” BMC PLANT BIOLOGY 16 (2016): n. pag. Print.