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BMP and Hedgehog regulate distinct AGM hematopoietic stem cells ex vivo

Mihaela Crisan, Parham Solaimani Kartalaei, Alex Neagu, Sofia Karkanpouna, Tomoko Yamada-Inagawa, Caterina Purini, Chris S Vink, Reinier van der Linden, Wilfred van Ijcken, Susana Marina Chuva de Sousa Lopes UGent, et al. (2016) STEM CELL REPORTS. 6(3). p.383-395
abstract
Hematopoietic stem cells (HSC), the self-renewing cells of the adult blood differentiation hierarchy, are generated during embryonic stages. The first HSCs are produced in the aorta-gonad-mesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production and expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. Transcriptomic analyses demonstrate that the two HSC types express distinct but overlapping genetic programs. These results revealing the bifurcation in HSC types at early embryonic stages in the AGM explant model suggest that their development is dependent upon the signaling molecules in the microenvironment.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
BONE MORPHOGENETIC PROTEIN-4, MOUSE EMBRYO, DEFINITIVE HEMATOPOIESIS, DORSAL AORTA, FETAL LIVER, LEUKEMIA, EXPRESSION, PATHWAY, REGION, IDENTIFICATION
journal title
STEM CELL REPORTS
Stem Cell Rep.
volume
6
issue
3
pages
383 - 395
Web of Science type
Article
Web of Science id
000373791600011
JCR category
CELL & TISSUE ENGINEERING
JCR impact factor
7.338 (2016)
JCR rank
3/21 (2016)
JCR quartile
1 (2016)
ISSN
2213-6711
DOI
10.1016/j.stemcr.2016.01.016
language
English
UGent publication?
no
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8500623
handle
http://hdl.handle.net/1854/LU-8500623
date created
2017-01-06 13:17:42
date last changed
2017-05-23 14:21:54
@article{8500623,
  abstract     = {Hematopoietic stem cells (HSC), the self-renewing cells of the adult blood differentiation hierarchy, are generated during embryonic stages. The first HSCs are produced in the aorta-gonad-mesonephros (AGM) region of the embryo through endothelial to a hematopoietic transition. BMP4 and Hedgehog affect their production and expansion, but it is unknown whether they act to affect the same HSCs. In this study using the BRE GFP reporter mouse strain that identifies BMP/Smad-activated cells, we find that the AGM harbors two types of adult-repopulating HSCs upon explant culture: One type is BMP-activated and the other is a non-BMP-activated HSC type that is indirectly controlled by Hedgehog signaling through the VEGF pathway. Transcriptomic analyses demonstrate that the two HSC types express distinct but overlapping genetic programs. These results revealing the bifurcation in HSC types at early embryonic stages in the AGM explant model suggest that their development is dependent upon the signaling molecules in the microenvironment.},
  author       = {Crisan, Mihaela and Kartalaei, Parham Solaimani and Neagu, Alex and Karkanpouna, Sofia and Yamada-Inagawa, Tomoko and Purini, Caterina and Vink, Chris S and van der Linden, Reinier and van Ijcken, Wilfred and Chuva de Sousa Lopes, Susana Marina and Monteiro, Rui and Mummery, Christine and Dzierzak, Elaine},
  issn         = {2213-6711},
  journal      = {STEM CELL REPORTS},
  keyword      = {BONE MORPHOGENETIC PROTEIN-4,MOUSE EMBRYO,DEFINITIVE HEMATOPOIESIS,DORSAL AORTA,FETAL LIVER,LEUKEMIA,EXPRESSION,PATHWAY,REGION,IDENTIFICATION},
  language     = {eng},
  number       = {3},
  pages        = {383--395},
  title        = {BMP and Hedgehog regulate distinct AGM hematopoietic stem cells ex vivo},
  url          = {http://dx.doi.org/10.1016/j.stemcr.2016.01.016},
  volume       = {6},
  year         = {2016},
}

Chicago
Crisan, Mihaela, Parham Solaimani Kartalaei, Alex Neagu, Sofia Karkanpouna, Tomoko Yamada-Inagawa, Caterina Purini, Chris S Vink, et al. 2016. “BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo.” Stem Cell Reports 6 (3): 383–395.
APA
Crisan, M., Kartalaei, P. S., Neagu, A., Karkanpouna, S., Yamada-Inagawa, T., Purini, C., Vink, C. S., et al. (2016). BMP and Hedgehog regulate distinct AGM hematopoietic stem cells ex vivo. STEM CELL REPORTS, 6(3), 383–395.
Vancouver
1.
Crisan M, Kartalaei PS, Neagu A, Karkanpouna S, Yamada-Inagawa T, Purini C, et al. BMP and Hedgehog regulate distinct AGM hematopoietic stem cells ex vivo. STEM CELL REPORTS. 2016;6(3):383–95.
MLA
Crisan, Mihaela, Parham Solaimani Kartalaei, Alex Neagu, et al. “BMP and Hedgehog Regulate Distinct AGM Hematopoietic Stem Cells Ex Vivo.” STEM CELL REPORTS 6.3 (2016): 383–395. Print.