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Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora

Nabil El Aila UGent, Inge Tency UGent, Geert Claeys UGent, Hans Verstraelen UGent, Bart Saerens UGent, Guido Lopes dos Santos Santiago UGent, Ellen De Backer UGent, Piet Cools UGent, Marleen Temmerman UGent and Rita Verhelst UGent, et al. (2009) BMC INFECTIOUS DISEASES. 9.
abstract
Background: The vaginal microflora is important for maintaining vaginal health and preventing infections of the reproductive tract. The rectum has been suggested as the major source for the colonisation of the vaginal econiche. Methods: To establish whether the rectum can serve as a possible bacterial reservoir for colonisation of the vaginal econiche, we cultured vaginal and rectal specimens from pregnant women at 35-37 weeks of gestation, identified the isolates to the species level with tRNA intergenic length polymorphism analysis (tDNA-PCR) and genotyped the isolates for those subjects from which the same species was isolated simultaneously vaginally and rectally, by RAPD-analysis. One vaginal and one rectal swab were collected from a total of each of 132 pregnant women at 3537 weeks of gestation. Swabs were cultured on Columbia CNA agar and MRS agar. For each subject 4 colonies were selected for each of both sites, i.e. 8 colonies in total. Results: Among the 844 isolates that could be identified by tDNA-PCR, a total of 63 bacterial species were present, 9 (14%) only vaginally, 26 (41%) only rectally, and 28 (44%) in both vagina and rectum. A total of 121 (91.6%) of 132 vaginal samples and 51 (38.6%) of 132 rectal samples were positive for lactobacilli. L. crispatus was the most frequently isolated Lactobacillus species from the vagina (40% of the subjects were positive), followed by L. jensenii (32%), L. gasseri (30%) and L. iners (11%). L. gasseri was the most frequently isolated Lactobacillus species from the rectum (15%), followed by L. jensenii (12%), L. crispatus (11%) and L. iners (2%). A total of 47 pregnant women carried the same species vaginally and rectally. This resulted in 50 vaginal/rectal pairs of the same species, for a total of eight different species. For 34 of the 50 species pairs (68%), isolates with the same genotype were present vaginally and rectally and a high level of genotypic diversity within species per subject was also established. Conclusion: It can be concluded that there is a certain degree of correspondence between the vaginal and rectal microflora, not only with regard to species composition but also with regard to strain identity between vaginal and rectal isolates. These results support the hypothesis that the rectal microflora serves as a reservoir for colonisation of the vaginal econiche.
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author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
LACTOBACILLUS-ACIDOPHILUS, ATOPOBIUM-VAGINAE, MICROBIAL COMMUNITIES, GARDNERELLA-VAGINALIS, HUMAN FECAL FLORA, IMMUNODEFICIENCY-VIRUS TYPE-1, HYDROGEN-PEROXIDE PRODUCTION, HEALTHY WOMEN, TDNA-PCR, VAGINOSIS
journal title
BMC INFECTIOUS DISEASES
BMC Infect. Dis.
volume
9
pages
14 pages
publisher
BIOMED CENTRAL LTD
place of publication
LONDON
Web of Science type
Article
Web of Science id
000271924700001
JCR category
INFECTIOUS DISEASES
JCR impact factor
2.55 (2009)
JCR rank
27/57 (2009)
JCR quartile
2 (2009)
ISSN
1471-2334
DOI
10.1186/1471-2334-9-167
language
English
UGent publication?
yes
classification
A1
id
846950
handle
http://hdl.handle.net/1854/LU-846950
date created
2010-02-01 10:32:48
date last changed
2012-04-06 11:14:06
@article{846950,
  abstract     = {Background: The vaginal microflora is important for maintaining vaginal health and preventing infections of the reproductive tract. The rectum has been suggested as the major source for the colonisation of the vaginal econiche.
Methods: To establish whether the rectum can serve as a possible bacterial reservoir for colonisation of the vaginal econiche, we cultured vaginal and rectal specimens from pregnant women at 35-37 weeks of gestation, identified the isolates to the species level with tRNA intergenic length polymorphism analysis (tDNA-PCR) and genotyped the isolates for those subjects from which the same species was isolated simultaneously vaginally and rectally, by RAPD-analysis.

One vaginal and one rectal swab were collected from a total of each of 132 pregnant women at 3537 weeks of gestation. Swabs were cultured on Columbia CNA agar and MRS agar. For each subject 4 colonies were selected for each of both sites, i.e. 8 colonies in total.

Results: Among the 844 isolates that could be identified by tDNA-PCR, a total of 63 bacterial species were present, 9 (14\%) only vaginally, 26 (41\%) only rectally, and 28 (44\%) in both vagina and rectum. A total of 121 (91.6\%) of 132 vaginal samples and 51 (38.6\%) of 132 rectal samples were positive for lactobacilli. L. crispatus was the most frequently isolated Lactobacillus species from the vagina (40\% of the subjects were positive), followed by L. jensenii (32\%), L. gasseri (30\%) and L. iners (11\%). L. gasseri was the most frequently isolated Lactobacillus species from the rectum (15\%), followed by L. jensenii (12\%), L. crispatus (11\%) and L. iners (2\%).

A total of 47 pregnant women carried the same species vaginally and rectally. This resulted in 50 vaginal/rectal pairs of the same species, for a total of eight different species. For 34 of the 50 species pairs (68\%), isolates with the same genotype were present vaginally and rectally and a high level of genotypic diversity within species per subject was also established.

Conclusion: It can be concluded that there is a certain degree of correspondence between the vaginal and rectal microflora, not only with regard to species composition but also with regard to strain identity between vaginal and rectal isolates.

These results support the hypothesis that the rectal microflora serves as a reservoir for colonisation of the vaginal econiche.},
  author       = {El Aila, Nabil and Tency, Inge and Claeys, Geert and Verstraelen, Hans and Saerens, Bart and Lopes dos Santos Santiago, Guido and De Backer, Ellen and Cools, Piet and Temmerman, Marleen and Verhelst, Rita and Vaneechoutte, Mario},
  issn         = {1471-2334},
  journal      = {BMC INFECTIOUS DISEASES},
  keyword      = {LACTOBACILLUS-ACIDOPHILUS,ATOPOBIUM-VAGINAE,MICROBIAL COMMUNITIES,GARDNERELLA-VAGINALIS,HUMAN FECAL FLORA,IMMUNODEFICIENCY-VIRUS TYPE-1,HYDROGEN-PEROXIDE PRODUCTION,HEALTHY WOMEN,TDNA-PCR,VAGINOSIS},
  language     = {eng},
  pages        = {14},
  publisher    = {BIOMED CENTRAL LTD},
  title        = {Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora},
  url          = {http://dx.doi.org/10.1186/1471-2334-9-167},
  volume       = {9},
  year         = {2009},
}

Chicago
El Aila, Nabil, Inge Tency, Geert Claeys, Hans Verstraelen, Bart Saerens, Guido Lopes dos Santos Santiago, Ellen De Backer, et al. 2009. “Identification and Genotyping of Bacteria from Paired Vaginal and Rectal Samples from Pregnant Women Indicates Similarity Between Vaginal and Rectal Microflora.” Bmc Infectious Diseases 9.
APA
El Aila, N., Tency, I., Claeys, G., Verstraelen, H., Saerens, B., Lopes dos Santos Santiago, G., De Backer, E., et al. (2009). Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora. BMC INFECTIOUS DISEASES, 9.
Vancouver
1.
El Aila N, Tency I, Claeys G, Verstraelen H, Saerens B, Lopes dos Santos Santiago G, et al. Identification and genotyping of bacteria from paired vaginal and rectal samples from pregnant women indicates similarity between vaginal and rectal microflora. BMC INFECTIOUS DISEASES. LONDON: BIOMED CENTRAL LTD; 2009;9.
MLA
El Aila, Nabil, Inge Tency, Geert Claeys, et al. “Identification and Genotyping of Bacteria from Paired Vaginal and Rectal Samples from Pregnant Women Indicates Similarity Between Vaginal and Rectal Microflora.” BMC INFECTIOUS DISEASES 9 (2009): n. pag. Print.