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The ang3 mutation identified the ribosomal protein gene RPL5B with a role in cell expansion during organ growth

Annemie Van Minnebruggen UGent, Pia Neyt UGent, Steven De Groeve UGent, Griet Coussens UGent, Maria Rosa Ponce, José Luis Micol and Maria Van Lijsebettens UGent (2010) PHYSIOLOGIA PLANTARUM. 138(1). p.91-101
abstract
The role of translation in the regulation of higher plant growth and development is not well understood. Mutational analysis is a powerful tool to identify and study the function of genes related to a biological process, such as growth. Here we analyzed functionally the angusta3 (ang3) narrow leaf mutant. The AG3 gene was cloned by fine mapping combined with candidate gene sequencing and it corresponded to the ribosomal protein gene RPL5B. Based on amino acid sequence homology, promoter DNA sequence homology and in silico gene expression analysis, RPL5B was found to be putatively functionally redundant with RPL5A. The morphological analysis of ang3 mutants showed that the leaf lamina area was significantly reduced from the third rosette leaf on, mainly because of decreased width. Cellular analysis of the abaxial epidermal cell layer of the third leaf indicated that the cell number in the mutant was similar to that of the wild type, but the cell size was significantly reduced. We postulate that the reduced cell expansion in the epidermis contributes to the narrow shape of ang3 leaves. Growth was also significantly impaired in hypocotyls and primary roots, hinting at a general role for RPL5B in organ growth, unrelated to dorsiventral axis formation. Comparison of the transcriptome of the shoot apices of the mutant and the wild type revealed a limited number of differentially expressed genes, such as MYB23 and MYB5, of which the lower expression in the ang3 mutant correlated with reduced trichome density. Our data suggest that translation is an important level of control of growth and development in plants.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
MUTAGENESIS, ENCODES, MUTANTS IDENTIFY, TRICHOME MORPHOGENESIS, ARABIDOPSIS-THALIANA, LEAF MORPHOGENESIS, EXPRESSION, PLANTS, REGION, L5
journal title
PHYSIOLOGIA PLANTARUM
Physiol. Plant.
volume
138
issue
1
pages
11 pages
Web of Science type
Article
Web of Science id
000272661700008
JCR category
PLANT SCIENCES
JCR impact factor
3.067 (2010)
JCR rank
26/185 (2010)
JCR quartile
1 (2010)
ISSN
0031-9317
DOI
10.1111/j.1399-3054.2009.01301.x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
846564
handle
http://hdl.handle.net/1854/LU-846564
date created
2010-01-29 18:30:15
date last changed
2012-09-19 14:04:03
@article{846564,
  abstract     = {The role of translation in the regulation of higher plant growth and development is not well understood. Mutational analysis is a powerful tool to identify and study the function of genes related to a biological process, such as growth. Here we analyzed functionally the angusta3 (ang3) narrow leaf mutant. The AG3 gene was cloned by fine mapping combined with candidate gene sequencing and it corresponded to the ribosomal protein gene RPL5B. Based on amino acid sequence homology, promoter DNA sequence homology and in silico gene expression analysis, RPL5B was found to be putatively functionally redundant with RPL5A. The morphological analysis of ang3 mutants showed that the leaf lamina area was significantly reduced from the third rosette leaf on, mainly because of decreased width. Cellular analysis of the abaxial epidermal cell layer of the third leaf indicated that the cell number in the mutant was similar to that of the wild type, but the cell size was significantly reduced. We postulate that the reduced cell expansion in the epidermis contributes to the narrow shape of ang3 leaves. Growth was also significantly impaired in hypocotyls and primary roots, hinting at a general role for RPL5B in organ growth, unrelated to dorsiventral axis formation. Comparison of the transcriptome of the shoot apices of the mutant and the wild type revealed a limited number of differentially expressed genes, such as MYB23 and MYB5, of which the lower expression in the ang3 mutant correlated with reduced trichome density. Our data suggest that translation is an important level of control of growth and development in plants.},
  author       = {Van Minnebruggen, Annemie and Neyt, Pia and De Groeve, Steven and Coussens, Griet and Ponce, Maria Rosa and Micol, Jos{\'e} Luis and Van Lijsebettens, Maria},
  issn         = {0031-9317},
  journal      = {PHYSIOLOGIA PLANTARUM},
  keyword      = {MUTAGENESIS,ENCODES,MUTANTS IDENTIFY,TRICHOME MORPHOGENESIS,ARABIDOPSIS-THALIANA,LEAF MORPHOGENESIS,EXPRESSION,PLANTS,REGION,L5},
  language     = {eng},
  number       = {1},
  pages        = {91--101},
  title        = {The ang3 mutation identified the ribosomal protein gene RPL5B with a role in cell expansion during organ growth},
  url          = {http://dx.doi.org/10.1111/j.1399-3054.2009.01301.x},
  volume       = {138},
  year         = {2010},
}

Chicago
Van Minnebruggen, Annemie, Pia Neyt, Steven De Groeve, Griet Coussens, Maria Rosa Ponce, José Luis Micol, and Maria Van Lijsebettens. 2010. “The Ang3 Mutation Identified the Ribosomal Protein Gene RPL5B with a Role in Cell Expansion During Organ Growth.” Physiologia Plantarum 138 (1): 91–101.
APA
Van Minnebruggen, A., Neyt, P., De Groeve, S., Coussens, G., Ponce, M. R., Micol, J. L., & Van Lijsebettens, M. (2010). The ang3 mutation identified the ribosomal protein gene RPL5B with a role in cell expansion during organ growth. PHYSIOLOGIA PLANTARUM, 138(1), 91–101.
Vancouver
1.
Van Minnebruggen A, Neyt P, De Groeve S, Coussens G, Ponce MR, Micol JL, et al. The ang3 mutation identified the ribosomal protein gene RPL5B with a role in cell expansion during organ growth. PHYSIOLOGIA PLANTARUM. 2010;138(1):91–101.
MLA
Van Minnebruggen, Annemie, Pia Neyt, Steven De Groeve, et al. “The Ang3 Mutation Identified the Ribosomal Protein Gene RPL5B with a Role in Cell Expansion During Organ Growth.” PHYSIOLOGIA PLANTARUM 138.1 (2010): 91–101. Print.