Ghent University Academic Bibliography

Advanced

Automated micronucleus (MN) scoring for population triage in case of large scale radiation events

Petra Willems UGent, Liezel August, Jacobus Slabbert, Horst Romm, Ursula Oestreicher, Hubert Thierens UGent and Anne Vral UGent (2010) INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 86(1). p.2-11
abstract
Purpose: In case of a large-scale radiation accident when hundreds of people may be exposed, it is important to distinguish the severely exposed individuals (>= 1 gray), who require early medical treatment, from those less exposed. The aim of our study was to develop a quick population triage method based on automated micronucleus (MN) scoring. Materials and methods: Using the MN software module developed by MetaSystems specifically for the Metafer4 platform, about 60 blood samples can be scored in one day. Standard dose response curves were determined for manual and automated MN scoring. Results: The automated MN assay results were closely correlated with MN yields obtained with the manual procedure. A dose of I Gy can be estimated with an uncertainty of 0.2 Gy. Corrections for false positives and false negatives by visual inspection of the image gallery did not result in an improved accuracy or reproducibility. To test the automated MN assay in a multicenter setting, an inter-laboratory comparison was performed whereby irradiated blood samples were processed in Ghent University (Belgium) and BfS (Bundesamt fuer Strahlenschutz; Germany). Both laboratories obtained comparable results. Conclusions: These results confirm the efficacy of the automated MN assay for fast population triage in a multicenter setting, in the case of large radiation accidents.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
cytogenetic assay, biological dosimetry, automated micronucleus (MN) assay, population triage, large-scale irradiation event, HUMAN-LYMPHOCYTES, CENTROMERE ASSAY, IMAGE-ANALYSIS, RADIOLOGICAL WORKERS, DETAILED DESCRIPTION, CHROMOSOME, DOSIMETRY, BIODOSIMETRY, ACCIDENTS, DAMAGE
journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Int. J. Radiat. Biol.
volume
86
issue
1
pages
2 - 11
Web of Science type
Article
Web of Science id
000274344300001
JCR category
NUCLEAR SCIENCE & TECHNOLOGY
JCR impact factor
1.861 (2010)
JCR rank
2/34 (2010)
JCR quartile
1 (2010)
ISSN
0955-3002
DOI
10.3109/09553000903264481
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
844095
handle
http://hdl.handle.net/1854/LU-844095
date created
2010-01-28 12:49:40
date last changed
2011-07-12 15:30:55
@article{844095,
  abstract     = {Purpose: In case of a large-scale radiation accident when hundreds of people may be exposed, it is important to distinguish the severely exposed individuals ({\textrangle}= 1 gray), who require early medical treatment, from those less exposed. The aim of our study was to develop a quick population triage method based on automated micronucleus (MN) scoring.
Materials and methods: Using the MN software module developed by MetaSystems specifically for the Metafer4 platform, about 60 blood samples can be scored in one day. Standard dose response curves were determined for manual and automated MN scoring.
Results: The automated MN assay results were closely correlated with MN yields obtained with the manual procedure. A dose of I Gy can be estimated with an uncertainty of 0.2 Gy. Corrections for false positives and false negatives by visual inspection of the image gallery did not result in an improved accuracy or reproducibility. To test the automated MN assay in a multicenter setting, an inter-laboratory comparison was performed whereby irradiated blood samples were processed in Ghent University (Belgium) and BfS (Bundesamt fuer Strahlenschutz; Germany). Both laboratories obtained comparable results.
Conclusions: These results confirm the efficacy of the automated MN assay for fast population triage in a multicenter setting, in the case of large radiation accidents.},
  author       = {Willems, Petra and August, Liezel and Slabbert, Jacobus and Romm, Horst and Oestreicher, Ursula and Thierens, Hubert and Vral, Anne},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {cytogenetic assay,biological dosimetry,automated micronucleus (MN) assay,population triage,large-scale irradiation event,HUMAN-LYMPHOCYTES,CENTROMERE ASSAY,IMAGE-ANALYSIS,RADIOLOGICAL WORKERS,DETAILED DESCRIPTION,CHROMOSOME,DOSIMETRY,BIODOSIMETRY,ACCIDENTS,DAMAGE},
  language     = {eng},
  number       = {1},
  pages        = {2--11},
  title        = {Automated micronucleus (MN) scoring for population triage in case of large scale radiation events},
  url          = {http://dx.doi.org/10.3109/09553000903264481},
  volume       = {86},
  year         = {2010},
}

Chicago
Willems, Petra, Liezel August, Jacobus Slabbert, Horst Romm, Ursula Oestreicher, Hubert Thierens, and Anne Vral. 2010. “Automated Micronucleus (MN) Scoring for Population Triage in Case of Large Scale Radiation Events.” International Journal of Radiation Biology 86 (1): 2–11.
APA
Willems, P., August, L., Slabbert, J., Romm, H., Oestreicher, U., Thierens, H., & Vral, A. (2010). Automated micronucleus (MN) scoring for population triage in case of large scale radiation events. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 86(1), 2–11.
Vancouver
1.
Willems P, August L, Slabbert J, Romm H, Oestreicher U, Thierens H, et al. Automated micronucleus (MN) scoring for population triage in case of large scale radiation events. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2010;86(1):2–11.
MLA
Willems, Petra, Liezel August, Jacobus Slabbert, et al. “Automated Micronucleus (MN) Scoring for Population Triage in Case of Large Scale Radiation Events.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 86.1 (2010): 2–11. Print.