Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation
- Author
- Laurence Beels (UGent) , Joke Werbrouck (UGent) and Hubert Thierens (UGent)
- Organization
- Abstract
- Purpose: Dose response and repair kinetics of γ-H2AX foci in T-lymphocytes were investigated in the low dose range after in vitro irradiation of whole blood and T-lymphocytes with 100kVp X-rays and 60Co γ-rays. Materials and methods: Whole blood or isolated T-lymphocytes were irradiated in vitro and γ-H2AX foci were scored. Dose response was determined in the 0–500mGy dose range. Foci kinetics were studied at doses of 5mGy and 200mGy up to 24h post-irradiation. Results: After X-irradiation, the dose response for whole blood shows a biphasic behaviour with a low dose hypersensitivity, which is less pronounced for isolated T-lymphocytes. In contrast, γ-radiation shows a linear dose response for both irradiation conditions. Concerning repair kinetics, delayed repair was found after X-ray whole blood irradiation (5mGy and 200mGy) with 40% of the foci persisting 24h post-irradiation. This number of foci is reduced to 10% after irradiation of isolated T-lymphocytes with 200mGy X-rays. On the contrary, γ-H2AX foci are reduced to background levels 24h post-irradiation with 200mGy 60Co γ-rays. Conclusions: γ-H2AX foci response and repair kinetics depend on irradiation conditions and radiation quality, possibly linked to bystander response.
- Keywords
- repair kinetics, γ-H2AX foci, dose response, DOUBLE-STRAND BREAKS, HISTONE H2AX PHOSPHORYLATION, CLONOGENIC SURVIVAL, IONIZING-RADIATION, DNA-DAMAGE, EXPOSURE, CELLS, RADIOTHERAPY, SITES, VIVO
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Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-842803
- MLA
- Beels, Laurence, et al. “Dose Response and Repair Kinetics of γ-H2AX Foci Induced by in Vitro Irradiation of Whole Blood and T-Lymphocytes with X- and γ-Radiation.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, vol. 86, no. 9, 2010, pp. 760–68, doi:10.3109/09553002.2010.484479.
- APA
- Beels, L., Werbrouck, J., & Thierens, H. (2010). Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 86(9), 760–768. https://doi.org/10.3109/09553002.2010.484479
- Chicago author-date
- Beels, Laurence, Joke Werbrouck, and Hubert Thierens. 2010. “Dose Response and Repair Kinetics of γ-H2AX Foci Induced by in Vitro Irradiation of Whole Blood and T-Lymphocytes with X- and γ-Radiation.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 86 (9): 760–68. https://doi.org/10.3109/09553002.2010.484479.
- Chicago author-date (all authors)
- Beels, Laurence, Joke Werbrouck, and Hubert Thierens. 2010. “Dose Response and Repair Kinetics of γ-H2AX Foci Induced by in Vitro Irradiation of Whole Blood and T-Lymphocytes with X- and γ-Radiation.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 86 (9): 760–768. doi:10.3109/09553002.2010.484479.
- Vancouver
- 1.Beels L, Werbrouck J, Thierens H. Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2010;86(9):760–8.
- IEEE
- [1]L. Beels, J. Werbrouck, and H. Thierens, “Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation,” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, vol. 86, no. 9, pp. 760–768, 2010.
@article{842803,
abstract = {{Purpose: Dose response and repair kinetics of γ-H2AX foci in T-lymphocytes were investigated in the low dose range after in vitro irradiation of whole blood and T-lymphocytes with 100kVp X-rays and 60Co γ-rays.
Materials and methods: Whole blood or isolated T-lymphocytes were irradiated in vitro and γ-H2AX foci were scored. Dose response was determined in the 0–500mGy dose range. Foci kinetics were studied at doses of 5mGy and 200mGy up to 24h post-irradiation.
Results: After X-irradiation, the dose response for whole blood shows a biphasic behaviour with a low dose hypersensitivity, which is less pronounced for isolated T-lymphocytes. In contrast, γ-radiation shows a linear dose response for both irradiation conditions. Concerning repair kinetics, delayed repair was found after X-ray whole blood irradiation (5mGy and 200mGy) with 40% of the foci persisting 24h post-irradiation. This number of foci is reduced to 10% after irradiation of isolated T-lymphocytes with 200mGy X-rays. On the contrary, γ-H2AX foci are reduced to background levels 24h post-irradiation with 200mGy 60Co γ-rays.
Conclusions: γ-H2AX foci response and repair kinetics depend on irradiation conditions and radiation quality, possibly linked to bystander response.}},
author = {{Beels, Laurence and Werbrouck, Joke and Thierens, Hubert}},
issn = {{0955-3002}},
journal = {{INTERNATIONAL JOURNAL OF RADIATION BIOLOGY}},
keywords = {{repair kinetics,γ-H2AX foci,dose response,DOUBLE-STRAND BREAKS,HISTONE H2AX PHOSPHORYLATION,CLONOGENIC SURVIVAL,IONIZING-RADIATION,DNA-DAMAGE,EXPOSURE,CELLS,RADIOTHERAPY,SITES,VIVO}},
language = {{eng}},
number = {{9}},
pages = {{760--768}},
title = {{Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation}},
url = {{http://doi.org/10.3109/09553002.2010.484479}},
volume = {{86}},
year = {{2010}},
}
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