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Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation

Laurence Beels UGent, Joke Werbrouck UGent and Hubert Thierens UGent (2010) INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 86(9). p.760-768
abstract
Purpose: Dose response and repair kinetics of γ-H2AX foci in T-lymphocytes were investigated in the low dose range after in vitro irradiation of whole blood and T-lymphocytes with 100kVp X-rays and 60Co γ-rays. Materials and methods: Whole blood or isolated T-lymphocytes were irradiated in vitro and γ-H2AX foci were scored. Dose response was determined in the 0–500mGy dose range. Foci kinetics were studied at doses of 5mGy and 200mGy up to 24h post-irradiation. Results: After X-irradiation, the dose response for whole blood shows a biphasic behaviour with a low dose hypersensitivity, which is less pronounced for isolated T-lymphocytes. In contrast, γ-radiation shows a linear dose response for both irradiation conditions. Concerning repair kinetics, delayed repair was found after X-ray whole blood irradiation (5mGy and 200mGy) with 40% of the foci persisting 24h post-irradiation. This number of foci is reduced to 10% after irradiation of isolated T-lymphocytes with 200mGy X-rays. On the contrary, γ-H2AX foci are reduced to background levels 24h post-irradiation with 200mGy 60Co γ-rays. Conclusions: γ-H2AX foci response and repair kinetics depend on irradiation conditions and radiation quality, possibly linked to bystander response.
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author
organization
alternative title
Dose response and repair kinetics of gamma-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and gamma-radiation
year
type
journalArticle (original)
publication status
published
subject
keyword
repair kinetics, γ-H2AX foci, dose response, DOUBLE-STRAND BREAKS, HISTONE H2AX PHOSPHORYLATION, CLONOGENIC SURVIVAL, IONIZING-RADIATION, DNA-DAMAGE, EXPOSURE, CELLS, RADIOTHERAPY, SITES, VIVO
journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Int. J. Radiat. Biol.
volume
86
issue
9
pages
760 - 768
Web of Science type
Article
Web of Science id
000281771100005
JCR category
NUCLEAR SCIENCE & TECHNOLOGY
JCR impact factor
1.861 (2010)
JCR rank
2/34 (2010)
JCR quartile
1 (2010)
ISSN
0955-3002
DOI
10.3109/09553002.2010.484479
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
842803
handle
http://hdl.handle.net/1854/LU-842803
date created
2010-01-27 14:02:12
date last changed
2011-07-12 15:24:28
@article{842803,
  abstract     = {Purpose: Dose response and repair kinetics of \ensuremath{\gamma}-H2AX foci in T-lymphocytes were investigated in the low dose range after in vitro irradiation of whole blood and T-lymphocytes with 100kVp X-rays and 60Co \ensuremath{\gamma}-rays.
Materials and methods: Whole blood or isolated T-lymphocytes were irradiated in vitro and \ensuremath{\gamma}-H2AX foci were scored. Dose response was determined in the 0--500mGy dose range. Foci kinetics were studied at doses of 5mGy and 200mGy up to 24h post-irradiation.
Results: After X-irradiation, the dose response for whole blood shows a biphasic behaviour with a low dose hypersensitivity, which is less pronounced for isolated T-lymphocytes. In contrast, \ensuremath{\gamma}-radiation shows a linear dose response for both irradiation conditions. Concerning repair kinetics, delayed repair was found after X-ray whole blood irradiation (5mGy and 200mGy) with 40\% of the foci persisting 24h post-irradiation. This number of foci is reduced to 10\% after irradiation of isolated T-lymphocytes with 200mGy X-rays. On the contrary, \ensuremath{\gamma}-H2AX foci are reduced to background levels 24h post-irradiation with 200mGy 60Co \ensuremath{\gamma}-rays.
Conclusions:  \ensuremath{\gamma}-H2AX foci response and repair kinetics depend on irradiation conditions and radiation quality, possibly linked to bystander response.},
  author       = {Beels, Laurence and Werbrouck, Joke and Thierens, Hubert},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {repair kinetics,\ensuremath{\gamma}-H2AX foci,dose response,DOUBLE-STRAND BREAKS,HISTONE H2AX PHOSPHORYLATION,CLONOGENIC SURVIVAL,IONIZING-RADIATION,DNA-DAMAGE,EXPOSURE,CELLS,RADIOTHERAPY,SITES,VIVO},
  language     = {eng},
  number       = {9},
  pages        = {760--768},
  title        = {Dose response and repair kinetics of \ensuremath{\gamma}-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and \ensuremath{\gamma}-radiation},
  url          = {http://dx.doi.org/10.3109/09553002.2010.484479},
  volume       = {86},
  year         = {2010},
}

Chicago
Beels, Laurence, Joke Werbrouck, and Hubert Thierens. 2010. “Dose Response and Repair Kinetics of γ-H2AX Foci Induced by in Vitro Irradiation of Whole Blood and T-lymphocytes with X- and Γ-radiation.” International Journal of Radiation Biology 86 (9): 760–768.
APA
Beels, L., Werbrouck, J., & Thierens, H. (2010). Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 86(9), 760–768.
Vancouver
1.
Beels L, Werbrouck J, Thierens H. Dose response and repair kinetics of γ-H2AX foci induced by in vitro irradiation of whole blood and T-lymphocytes with X- and γ-radiation. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2010;86(9):760–8.
MLA
Beels, Laurence, Joke Werbrouck, and Hubert Thierens. “Dose Response and Repair Kinetics of γ-H2AX Foci Induced by in Vitro Irradiation of Whole Blood and T-lymphocytes with X- and Γ-radiation.” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 86.9 (2010): 760–768. Print.