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A rapid and simple assay to determine pegylated erythropoietin in human serum

Tom Van Maerken UGent, Annemieke Dhondt UGent and Joris Delanghe UGent (2010) JOURNAL OF APPLIED PHYSIOLOGY. 108(4). p.800-803
abstract
Stimulation of erythropoiesis by the third-generation erythropoietin drug, continuous erythropoietin receptor activator (CERA), a pegylated derivative of epoetin-beta, has provided valuable therapeutic benefits to patients suffering from renal anemia, but has also rapidly found application as an illicit performance-enhancing strategy in endurance sports. We present here a novel method for selective determination of CERA in serum, based on polyethylene glycol precipitation, followed by a commercial homogeneous immunoassay. The developed method was highly discriminating between serum samples from CERA-treated patients and control subjects, as the covalently linked polyethylene glycol chain in CERA strongly enhanced the solubility of the protein in a polyethylene glycol-containing medium. Intravenous administration of CERA could be detected for several weeks in the majority of subjects tested. This assay outperforms the currently available CERA detection methods in terms of simplicity, convenience, cost, and throughput, making it ideal as a screening tool for doping control.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
CERA, continuous erythropoietin receptor activator, doping, endurance sports, detection, POLYETHYLENE-GLYCOL PRECIPITATION, RECEPTOR ACTIVATOR, DOPING CONTROL, RECOMBINANT, BLOOD, URINE, PAGE
journal title
JOURNAL OF APPLIED PHYSIOLOGY
J. Appl. Physiol.
volume
108
issue
4
pages
800 - 803
Web of Science type
Article
Web of Science id
000276324200007
JCR category
SPORT SCIENCES
JCR impact factor
4.232 (2010)
JCR rank
2/79 (2010)
JCR quartile
1 (2010)
ISSN
8750-7587
DOI
10.1152/japplphysiol.01102.2009
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
835737
handle
http://hdl.handle.net/1854/LU-835737
date created
2010-01-24 00:37:18
date last changed
2011-07-13 09:49:09
@article{835737,
  abstract     = {Stimulation of erythropoiesis by the third-generation erythropoietin drug, continuous erythropoietin receptor activator (CERA), a pegylated derivative of epoetin-beta, has provided valuable therapeutic benefits to patients suffering from renal anemia, but has also rapidly found application as an illicit performance-enhancing strategy in endurance sports. We present here a novel method for selective determination of CERA in serum, based on polyethylene glycol precipitation, followed by a commercial homogeneous immunoassay. The developed method was highly discriminating between serum samples from CERA-treated patients and control subjects, as the covalently linked polyethylene glycol chain in CERA strongly enhanced the solubility of the protein in a polyethylene glycol-containing medium. Intravenous administration of CERA could be detected for several weeks in the majority of subjects tested. This assay outperforms the currently available CERA detection methods in terms of simplicity, convenience, cost, and throughput, making it ideal as a screening tool for doping control.},
  author       = {Van Maerken, Tom and Dhondt, Annemieke and Delanghe, Joris},
  issn         = {8750-7587},
  journal      = {JOURNAL OF APPLIED PHYSIOLOGY},
  keyword      = {CERA,continuous erythropoietin receptor activator,doping,endurance sports,detection,POLYETHYLENE-GLYCOL PRECIPITATION,RECEPTOR ACTIVATOR,DOPING CONTROL,RECOMBINANT,BLOOD,URINE,PAGE},
  language     = {eng},
  number       = {4},
  pages        = {800--803},
  title        = {A rapid and simple assay to determine pegylated erythropoietin in human serum},
  url          = {http://dx.doi.org/10.1152/japplphysiol.01102.2009},
  volume       = {108},
  year         = {2010},
}

Chicago
Van Maerken, Tom, Annemieke Dhondt, and Joris Delanghe. 2010. “A Rapid and Simple Assay to Determine Pegylated Erythropoietin in Human Serum.” Journal of Applied Physiology 108 (4): 800–803.
APA
Van Maerken, T., Dhondt, A., & Delanghe, J. (2010). A rapid and simple assay to determine pegylated erythropoietin in human serum. JOURNAL OF APPLIED PHYSIOLOGY, 108(4), 800–803.
Vancouver
1.
Van Maerken T, Dhondt A, Delanghe J. A rapid and simple assay to determine pegylated erythropoietin in human serum. JOURNAL OF APPLIED PHYSIOLOGY. 2010;108(4):800–3.
MLA
Van Maerken, Tom, Annemieke Dhondt, and Joris Delanghe. “A Rapid and Simple Assay to Determine Pegylated Erythropoietin in Human Serum.” JOURNAL OF APPLIED PHYSIOLOGY 108.4 (2010): 800–803. Print.