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Gamma radiation alters the ultrastructure in tissue-engineered heart valve scaffolds

Pamela Somers UGent, Claude Cuvelier UGent, Filip De Somer UGent, Maria Cornelissen UGent, Eric Cox UGent, Marc Verloo UGent, Koen Chiers UGent and Guido Van Nooten UGent (2009) TISSUE ENGINEERING PART A. 15(11). p.3597-3604
abstract
OBJECTIVES: Xenogenic extracellular heart valve matrices have been suggested as scaffolds for tissue engineering. However, these matrices are immunogenic and stimulate an intense cell-mediated immune response and calcification. Mitigating the immunogenicity was attempted by different doses of gamma irradiation. METHODS: Mechanical properties of gamma-irradiated porcine matrices and control matrices (nonirradiated) were examined by tensile strength testing. Irradiated matrices (1, 10, 50, and 100 gray [Gy]) and control matrices were implanted subcutaneously in Wistar rats (n = 20). After 24 h, 1, 2, 3, and 4 weeks the explants were examined by light microscopy and transmission electron microscopy. Calcium (Ca) content was determined using inductively coupled plasma-mass spectrometry. Antibody reaction against porcine tissue in the rat serum was determined. RESULTS: Tensile strength increased in irradiated matrices at the expense of elasticity. Ten gray-irradiated leaflets showed minimal lymphocytic inflammatory infiltration with preservation of ultrastructure. Ca levels after 2 weeks were as follows: control (0 Gy), 388 +/- 264 microg/mg; 1 Gy, 240 +/- 95 microg/mg; 10 Gy, 188 +/- 54 microg/mg; 50 Gy, 289 +/- 94 microg/mg; 100 Gy, 651 +/- 57 microg/mg. All implants still elicit an antibody immunoglobulin G reaction. CONCLUSIONS: Exposure to 10 Gy gamma irradiation reduces lymphocytic inflammatory infiltrates and Ca levels in acellular porcine matrices with preservation of structural integrity. This could prolong the durability of these matrices.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Extracellular Matrix, Cross-linker, Heart Valve, Immune Reponse, Modulation, Gamma-radiation
journal title
TISSUE ENGINEERING PART A
Tissue Eng. Part A
volume
15
issue
11
pages
3597 - 3604
Web of Science type
Article
Web of Science id
000271746800034
ISSN
1937-3341
DOI
10.1089/ten.tea.2008.0690
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
835622
handle
http://hdl.handle.net/1854/LU-835622
date created
2010-01-23 13:10:18
date last changed
2011-12-13 14:02:37
@article{835622,
  abstract     = {OBJECTIVES: Xenogenic extracellular heart valve matrices have been suggested as scaffolds for tissue engineering. However, these matrices are immunogenic and stimulate an intense cell-mediated immune response and calcification. Mitigating the immunogenicity was attempted by different doses of gamma irradiation. METHODS: Mechanical properties of gamma-irradiated porcine matrices and control matrices (nonirradiated) were examined by tensile strength testing. Irradiated matrices (1, 10, 50, and 100 gray [Gy]) and control matrices were implanted subcutaneously in Wistar rats (n = 20). After 24 h, 1, 2, 3, and 4 weeks the explants were examined by light microscopy and transmission electron microscopy. Calcium (Ca) content was determined using inductively coupled plasma-mass spectrometry. Antibody reaction against porcine tissue in the rat serum was determined. RESULTS: Tensile strength increased in irradiated matrices at the expense of elasticity. Ten gray-irradiated leaflets showed minimal lymphocytic inflammatory infiltration with preservation of ultrastructure. Ca levels after 2 weeks were as follows: control (0 Gy), 388 +/- 264 microg/mg; 1 Gy, 240 +/- 95 microg/mg; 10 Gy, 188 +/- 54 microg/mg; 50 Gy, 289 +/- 94 microg/mg; 100 Gy, 651 +/- 57 microg/mg. All implants still elicit an antibody immunoglobulin G reaction. CONCLUSIONS: Exposure to 10 Gy gamma irradiation reduces lymphocytic inflammatory infiltrates and Ca levels in acellular porcine matrices with preservation of structural integrity. This could prolong the durability of these matrices.},
  author       = {Somers, Pamela and Cuvelier, Claude and De Somer, Filip and Cornelissen, Maria and Cox, Eric and Verloo, Marc and Chiers, Koen and Van Nooten, Guido},
  issn         = {1937-3341},
  journal      = {TISSUE ENGINEERING PART A},
  keyword      = {Extracellular Matrix,Cross-linker,Heart Valve,Immune Reponse,Modulation,Gamma-radiation},
  language     = {eng},
  number       = {11},
  pages        = {3597--3604},
  title        = {Gamma radiation alters the ultrastructure in tissue-engineered heart valve scaffolds},
  url          = {http://dx.doi.org/10.1089/ten.tea.2008.0690},
  volume       = {15},
  year         = {2009},
}

Chicago
Somers, Pamela, Claude Cuvelier, Filip De Somer, Maria Cornelissen, Eric Cox, Marc Verloo, Koen Chiers, and Guido Van Nooten. 2009. “Gamma Radiation Alters the Ultrastructure in Tissue-engineered Heart Valve Scaffolds.” Tissue Engineering Part A 15 (11): 3597–3604.
APA
Somers, P., Cuvelier, C., De Somer, F., Cornelissen, M., Cox, E., Verloo, M., Chiers, K., et al. (2009). Gamma radiation alters the ultrastructure in tissue-engineered heart valve scaffolds. TISSUE ENGINEERING PART A, 15(11), 3597–3604.
Vancouver
1.
Somers P, Cuvelier C, De Somer F, Cornelissen M, Cox E, Verloo M, et al. Gamma radiation alters the ultrastructure in tissue-engineered heart valve scaffolds. TISSUE ENGINEERING PART A. 2009;15(11):3597–604.
MLA
Somers, Pamela, Claude Cuvelier, Filip De Somer, et al. “Gamma Radiation Alters the Ultrastructure in Tissue-engineered Heart Valve Scaffolds.” TISSUE ENGINEERING PART A 15.11 (2009): 3597–3604. Print.