Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs
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- Pieter Van Vlierberghe (UGent) , An De Weer, Pieter Mestdagh (UGent) , Tom Feys, Katleen De Preter (UGent) , Anne De Paepe (UGent) , Kathleen Lambein (UGent) , Jo Vandesompele (UGent) , Nadine Van Roy (UGent) , Bruno Verhasselt (UGent) , Bruce Poppe (UGent) and Franki Speleman (UGent)
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- Abstract
- Classical Hodgkin lymphoma (cHL) is characterized by the presence of malignant Hodgkin and Reed Sternberg (HRS) cells. The scarcity of tumour cells in lymphoma biopsies has hampered genetic analyses of HRS cells, including microRNA (miRNA) expression profiling. We determined the expression of 360 miRNAs in microdissected HRS cells from nine cHL patients. These miRNA profiles were compared to those from four cHL cell lines and CD77+ B-cells, yielding a distinct cHL signature of 12 over- and three underexpressed miRNAs. Our data suggest that miRNAs are implicated in the pathogenesis of Hodgkin lymphoma and prompt further investigations concerning their role in cHL.
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-833596
- MLA
- Van Vlierberghe, Pieter, et al. “Comparison of MiRNA Profiles of Microdissected Hodgkin/Reed-Sternberg Cells and Hodgkin Cell Lines versus CD77+ B-Cells Reveals a Distinct Subset of Differentially Expressed MiRNAs.” BRITISH JOURNAL OF HAEMATOLOGY, vol. 147, no. 5, 2009, pp. 686–90, doi:10.1111/j.1365-2141.2009.07909.x.
- APA
- Van Vlierberghe, P., De Weer, A., Mestdagh, P., Feys, T., De Preter, K., De Paepe, A., … Speleman, F. (2009). Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs. BRITISH JOURNAL OF HAEMATOLOGY, 147(5), 686–690. https://doi.org/10.1111/j.1365-2141.2009.07909.x
- Chicago author-date
- Van Vlierberghe, Pieter, An De Weer, Pieter Mestdagh, Tom Feys, Katleen De Preter, Anne De Paepe, Kathleen Lambein, et al. 2009. “Comparison of MiRNA Profiles of Microdissected Hodgkin/Reed-Sternberg Cells and Hodgkin Cell Lines versus CD77+ B-Cells Reveals a Distinct Subset of Differentially Expressed MiRNAs.” BRITISH JOURNAL OF HAEMATOLOGY 147 (5): 686–90. https://doi.org/10.1111/j.1365-2141.2009.07909.x.
- Chicago author-date (all authors)
- Van Vlierberghe, Pieter, An De Weer, Pieter Mestdagh, Tom Feys, Katleen De Preter, Anne De Paepe, Kathleen Lambein, Jo Vandesompele, Nadine Van Roy, Bruno Verhasselt, Bruce Poppe, and Franki Speleman. 2009. “Comparison of MiRNA Profiles of Microdissected Hodgkin/Reed-Sternberg Cells and Hodgkin Cell Lines versus CD77+ B-Cells Reveals a Distinct Subset of Differentially Expressed MiRNAs.” BRITISH JOURNAL OF HAEMATOLOGY 147 (5): 686–690. doi:10.1111/j.1365-2141.2009.07909.x.
- Vancouver
- 1.Van Vlierberghe P, De Weer A, Mestdagh P, Feys T, De Preter K, De Paepe A, et al. Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs. BRITISH JOURNAL OF HAEMATOLOGY. 2009;147(5):686–90.
- IEEE
- [1]P. Van Vlierberghe et al., “Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs,” BRITISH JOURNAL OF HAEMATOLOGY, vol. 147, no. 5, pp. 686–690, 2009.
@article{833596, abstract = {{Classical Hodgkin lymphoma (cHL) is characterized by the presence of malignant Hodgkin and Reed Sternberg (HRS) cells. The scarcity of tumour cells in lymphoma biopsies has hampered genetic analyses of HRS cells, including microRNA (miRNA) expression profiling. We determined the expression of 360 miRNAs in microdissected HRS cells from nine cHL patients. These miRNA profiles were compared to those from four cHL cell lines and CD77+ B-cells, yielding a distinct cHL signature of 12 over- and three underexpressed miRNAs. Our data suggest that miRNAs are implicated in the pathogenesis of Hodgkin lymphoma and prompt further investigations concerning their role in cHL.}}, author = {{Van Vlierberghe, Pieter and De Weer, An and Mestdagh, Pieter and Feys, Tom and De Preter, Katleen and De Paepe, Anne and Lambein, Kathleen and Vandesompele, Jo and Van Roy, Nadine and Verhasselt, Bruno and Poppe, Bruce and Speleman, Franki}}, issn = {{0007-1048}}, journal = {{BRITISH JOURNAL OF HAEMATOLOGY}}, language = {{eng}}, number = {{5}}, pages = {{686--690}}, title = {{Comparison of miRNA profiles of microdissected Hodgkin/Reed-Sternberg cells and Hodgkin cell lines versus CD77+ B-cells reveals a distinct subset of differentially expressed miRNAs}}, url = {{http://doi.org/10.1111/j.1365-2141.2009.07909.x}}, volume = {{147}}, year = {{2009}}, }
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