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In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatography

Bart Ghesquière UGent, Niklaas Colaert UGent, Kenny Helsens UGent, Lien Dejager UGent, Caroline Vanhaute, Katleen Verleysen, Koen Kas UGent, Evy Timmerman UGent, Marc Goethals UGent and Claude Libert UGent, et al. (2009) MOLECULAR & CELLULAR PROTEOMICS. 8(12). p.2642-2652
abstract
A new proteomics technique for analyzing 3-nitrotyrosine-containing peptides is presented here. This technique is based on the combined fractional diagonal chromatography peptide isolation procedures by which specific classes of peptides are isolated following a series of identical reverse-phase HPLC separation steps. Here dithionite is used to reduce 3-nitrotyrosine to 3-aminotyrosine peptides, which thereby become more hydrophilic. Our combined fractional diagonal chromatography technique was first applied to characterize tyrosine nitration in tetranitromethane-modified BSA and further led to a high quality list of 335 tyrosine nitration sites in 267 proteins in a peroxynitrite-treated lysate of human Jurkat cells. We then analyzed a serum sample of a C57BL6/J mouse in which septic shock was induced by intravenous Salmonella infection and identified six in vivo nitration events in four serum proteins, thereby illustrating that our technique is sufficiently sensitive to identify rare in vivo tyrosine nitration sites in a very complex background. Molecular & Cellular Proteomics 8: 2642-2652, 2009.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
NITRIC-OXIDE, PEROXYNITRITE, NITROTYROSINE, ALZHEIMERS-DISEASE, CONTAINING PEPTIDES, APOLIPOPROTEIN-A-I, CATALYZED OXIDATION, MASS-SPECTROMETRIC CHARACTERIZATION, PROTEOMIC IDENTIFICATION, SITES
journal title
MOLECULAR & CELLULAR PROTEOMICS
Mol. Cell. Proteomics
volume
8
issue
12
pages
11 pages
Web of Science type
Article
Web of Science id
000272711800003
JCR category
BIOCHEMICAL RESEARCH METHODS
JCR impact factor
8.791 (2009)
JCR rank
2/65 (2009)
JCR quartile
1 (2009)
ISSN
1535-9476
DOI
10.1074/mcp.M900259-MCP200
language
English
UGent publication?
yes
classification
A1
id
828083
handle
http://hdl.handle.net/1854/LU-828083
date created
2010-01-14 11:13:21
date last changed
2012-06-26 14:31:55
@article{828083,
  abstract     = {A new proteomics technique for analyzing 3-nitrotyrosine-containing peptides is presented here. This technique is based on the combined fractional diagonal chromatography peptide isolation procedures by which specific classes of peptides are isolated following a series of identical reverse-phase HPLC separation steps. Here dithionite is used to reduce 3-nitrotyrosine to 3-aminotyrosine peptides, which thereby become more hydrophilic. Our combined fractional diagonal chromatography technique was first applied to characterize tyrosine nitration in tetranitromethane-modified BSA and further led to a high quality list of 335 tyrosine nitration sites in 267 proteins in a peroxynitrite-treated lysate of human Jurkat cells. We then analyzed a serum sample of a C57BL6/J mouse in which septic shock was induced by intravenous Salmonella infection and identified six in vivo nitration events in four serum proteins, thereby illustrating that our technique is sufficiently sensitive to identify rare in vivo tyrosine nitration sites in a very complex background. Molecular \& Cellular Proteomics 8: 2642-2652, 2009.},
  author       = {Ghesqui{\`e}re, Bart and Colaert, Niklaas and Helsens, Kenny and Dejager, Lien and Vanhaute, Caroline and Verleysen, Katleen and Kas, Koen and Timmerman, Evy and Goethals, Marc and Libert, Claude and Vandekerckhove, Jo{\"e}l and Gevaert, Kris},
  issn         = {1535-9476},
  journal      = {MOLECULAR \& CELLULAR PROTEOMICS},
  keyword      = {NITRIC-OXIDE,PEROXYNITRITE,NITROTYROSINE,ALZHEIMERS-DISEASE,CONTAINING PEPTIDES,APOLIPOPROTEIN-A-I,CATALYZED OXIDATION,MASS-SPECTROMETRIC CHARACTERIZATION,PROTEOMIC IDENTIFICATION,SITES},
  language     = {eng},
  number       = {12},
  pages        = {2642--2652},
  title        = {In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatography},
  url          = {http://dx.doi.org/10.1074/mcp.M900259-MCP200},
  volume       = {8},
  year         = {2009},
}

Chicago
Ghesquière, Bart, Niklaas Colaert, Kenny Helsens, Lien Dejager, Caroline Vanhaute, Katleen Verleysen, Koen Kas, et al. 2009. “In Vitro and in Vivo Protein-bound Tyrosine Nitration Characterized by Diagonal Chromatography.” Molecular & Cellular Proteomics 8 (12): 2642–2652.
APA
Ghesquière, B., Colaert, N., Helsens, K., Dejager, L., Vanhaute, C., Verleysen, K., Kas, K., et al. (2009). In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatography. MOLECULAR & CELLULAR PROTEOMICS, 8(12), 2642–2652.
Vancouver
1.
Ghesquière B, Colaert N, Helsens K, Dejager L, Vanhaute C, Verleysen K, et al. In vitro and in vivo protein-bound tyrosine nitration characterized by diagonal chromatography. MOLECULAR & CELLULAR PROTEOMICS. 2009;8(12):2642–52.
MLA
Ghesquière, Bart, Niklaas Colaert, Kenny Helsens, et al. “In Vitro and in Vivo Protein-bound Tyrosine Nitration Characterized by Diagonal Chromatography.” MOLECULAR & CELLULAR PROTEOMICS 8.12 (2009): 2642–2652. Print.