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Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice

Jai-Wei Lee, Max Paape UGent and Xin Zhao (2003) VETERINARY RESEARCH. 34(3). p.307-316
abstract
Endotoxin, or lipopolysaccharide (LPS), is responsible for pathogenesis of infections induced by Gram-negative bacteria, such as E. coli. The cellular response to LPS is modulated by interactions among LPS, LPS-binding protein (LBP) and CD14. Accumulated evidence shows that the soluble form of CD14 (sCD14) competes with membrane-bound CD14 (mCD14) for LPS and plays a pivotal role in regulating bacterial infection and septic shock caused by Gram-negative bacteria. Recombinant bovine sCD14 (rbosCD14) was produced by transfected insect sf/9 cells and its biological function was evaluated in mice. Eighty-one 8-week old BALB/cj female mice were randomly assigned to two groups, and injected intraperitoneally with either LPS (8 mug/g of body weight, n = 41) or LPS plus rbosCD14 (6.8 mug/g of body weight, n = 40). Survival rate at 24 h after injection for mice injected with either LPS or LPS plus rbosCD14 was 30 and 72%, respectively (P < 0.01). At 48 h survival rate was 7 and 37%, respectively (P < 0.01). To investigate the protective effect of rbosCD14 on experimentally induced mastitis in mice, two abdominal contralateral mammary glands of 7 lactating BALB/cj mice were injected through the teat canal with 10-20 colony-forming units (CFU) of Escherichia coli. One gland simultaneously received rbosCD14 (6 mug) and the other saline. At 24 h after challenge, glands that received rbosCD14 had less swelling and hemorrhaging, significantly lower bacterial counts (P < 0.05) and lower concentrations of TNF-α (P < 0.05). Results indicate that rbosCD14 is biologically functional and reduces mortality in mice from endotoxin shock and severity of intramammary infection by E. coli.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
ENDOTOXIN, LPS, SEPTIC SHOCK, MAMMARY-GLAND, CYTOKINE PRODUCTION, CD14-DEFICIENT MICE, NECROSIS-FACTOR-ALPHA, GRAM-NEGATIVE BACTERIA, LIPOPOLYSACCHARIDE-BINDING-PROTEIN, TNF-alpha, Escherichia coli, mastitis, CD14, GROWTH
journal title
VETERINARY RESEARCH
Vet. Res.
volume
34
issue
3
pages
307 - 316
Web of Science type
Article
Web of Science id
000183880500006
JCR category
VETERINARY SCIENCES
JCR impact factor
1.609 (2003)
JCR rank
6/120 (2003)
JCR quartile
1 (2003)
ISSN
0928-4249
DOI
10.1051/vetres:2003006
language
English
UGent publication?
no
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
820718
handle
http://hdl.handle.net/1854/LU-820718
date created
2010-01-07 14:21:37
date last changed
2016-12-19 15:45:47
@article{820718,
  abstract     = {Endotoxin, or lipopolysaccharide (LPS), is responsible for pathogenesis of infections induced by Gram-negative bacteria, such as E. coli. The cellular response to LPS is modulated by interactions among LPS, LPS-binding protein (LBP) and CD14. Accumulated evidence shows that the soluble form of CD14 (sCD14) competes with membrane-bound CD14 (mCD14) for LPS and plays a pivotal role in regulating bacterial infection and septic shock caused by Gram-negative bacteria. Recombinant bovine sCD14 (rbosCD14) was produced by transfected insect sf/9 cells and its biological function was evaluated in mice. Eighty-one 8-week old BALB/cj female mice were randomly assigned to two groups, and injected intraperitoneally with either LPS (8 mug/g of body weight, n = 41) or LPS plus rbosCD14 (6.8 mug/g of body weight, n = 40). Survival rate at 24 h after injection for mice injected with either LPS or LPS plus rbosCD14 was 30 and 72\%, respectively (P {\textlangle} 0.01). At 48 h survival rate was 7 and 37\%, respectively (P {\textlangle} 0.01). To investigate the protective effect of rbosCD14 on experimentally induced mastitis in mice, two abdominal contralateral mammary glands of 7 lactating BALB/cj mice were injected through the teat canal with 10-20 colony-forming units (CFU) of Escherichia coli. One gland simultaneously received rbosCD14 (6 mug) and the other saline. At 24 h after challenge, glands that received rbosCD14 had less swelling and hemorrhaging, significantly lower bacterial counts (P {\textlangle} 0.05) and lower concentrations of TNF-\ensuremath{\alpha} (P {\textlangle} 0.05). Results indicate that rbosCD14 is biologically functional and reduces mortality in mice from endotoxin shock and severity of intramammary infection by E. coli.},
  author       = {Lee, Jai-Wei and Paape, Max and Zhao, Xin},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {ENDOTOXIN,LPS,SEPTIC SHOCK,MAMMARY-GLAND,CYTOKINE PRODUCTION,CD14-DEFICIENT MICE,NECROSIS-FACTOR-ALPHA,GRAM-NEGATIVE BACTERIA,LIPOPOLYSACCHARIDE-BINDING-PROTEIN,TNF-alpha,Escherichia coli,mastitis,CD14,GROWTH},
  language     = {eng},
  number       = {3},
  pages        = {307--316},
  title        = {Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice},
  url          = {http://dx.doi.org/10.1051/vetres:2003006},
  volume       = {34},
  year         = {2003},
}

Chicago
Lee, Jai-Wei, Max Paape, and Xin Zhao. 2003. “Recombinant Bovine Soluble CD14 Reduces Severity of Experimental Escherichia Coli Mastitis in Mice.” Veterinary Research 34 (3): 307–316.
APA
Lee, J.-W., Paape, M., & Zhao, X. (2003). Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice. VETERINARY RESEARCH, 34(3), 307–316.
Vancouver
1.
Lee J-W, Paape M, Zhao X. Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice. VETERINARY RESEARCH. 2003;34(3):307–16.
MLA
Lee, Jai-Wei, Max Paape, and Xin Zhao. “Recombinant Bovine Soluble CD14 Reduces Severity of Experimental Escherichia Coli Mastitis in Mice.” VETERINARY RESEARCH 34.3 (2003): 307–316. Print.