Advanced search
1 file | 1.52 MB

Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice

(2003) VETERINARY RESEARCH. 34(3). p.307-316
Author
Organization
Abstract
Endotoxin, or lipopolysaccharide (LPS), is responsible for pathogenesis of infections induced by Gram-negative bacteria, such as E. coli. The cellular response to LPS is modulated by interactions among LPS, LPS-binding protein (LBP) and CD14. Accumulated evidence shows that the soluble form of CD14 (sCD14) competes with membrane-bound CD14 (mCD14) for LPS and plays a pivotal role in regulating bacterial infection and septic shock caused by Gram-negative bacteria. Recombinant bovine sCD14 (rbosCD14) was produced by transfected insect sf/9 cells and its biological function was evaluated in mice. Eighty-one 8-week old BALB/cj female mice were randomly assigned to two groups, and injected intraperitoneally with either LPS (8 mug/g of body weight, n = 41) or LPS plus rbosCD14 (6.8 mug/g of body weight, n = 40). Survival rate at 24 h after injection for mice injected with either LPS or LPS plus rbosCD14 was 30 and 72%, respectively (P < 0.01). At 48 h survival rate was 7 and 37%, respectively (P < 0.01). To investigate the protective effect of rbosCD14 on experimentally induced mastitis in mice, two abdominal contralateral mammary glands of 7 lactating BALB/cj mice were injected through the teat canal with 10-20 colony-forming units (CFU) of Escherichia coli. One gland simultaneously received rbosCD14 (6 mug) and the other saline. At 24 h after challenge, glands that received rbosCD14 had less swelling and hemorrhaging, significantly lower bacterial counts (P < 0.05) and lower concentrations of TNF-α (P < 0.05). Results indicate that rbosCD14 is biologically functional and reduces mortality in mice from endotoxin shock and severity of intramammary infection by E. coli.
Keywords
ENDOTOXIN, LPS, SEPTIC SHOCK, MAMMARY-GLAND, CYTOKINE PRODUCTION, CD14-DEFICIENT MICE, NECROSIS-FACTOR-ALPHA, GRAM-NEGATIVE BACTERIA, LIPOPOLYSACCHARIDE-BINDING-PROTEIN, TNF-alpha, Escherichia coli, mastitis, CD14, GROWTH

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 1.52 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Lee, Jai-Wei, Max Paape, and Xin Zhao. 2003. “Recombinant Bovine Soluble CD14 Reduces Severity of Experimental Escherichia Coli Mastitis in Mice.” Veterinary Research 34 (3): 307–316.
APA
Lee, J.-W., Paape, M., & Zhao, X. (2003). Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice. VETERINARY RESEARCH, 34(3), 307–316.
Vancouver
1.
Lee J-W, Paape M, Zhao X. Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice. VETERINARY RESEARCH. 2003;34(3):307–16.
MLA
Lee, Jai-Wei, Max Paape, and Xin Zhao. “Recombinant Bovine Soluble CD14 Reduces Severity of Experimental Escherichia Coli Mastitis in Mice.” VETERINARY RESEARCH 34.3 (2003): 307–316. Print.
@article{820718,
  abstract     = {Endotoxin, or lipopolysaccharide (LPS), is responsible for pathogenesis of infections induced by Gram-negative bacteria, such as E. coli. The cellular response to LPS is modulated by interactions among LPS, LPS-binding protein (LBP) and CD14. Accumulated evidence shows that the soluble form of CD14 (sCD14) competes with membrane-bound CD14 (mCD14) for LPS and plays a pivotal role in regulating bacterial infection and septic shock caused by Gram-negative bacteria. Recombinant bovine sCD14 (rbosCD14) was produced by transfected insect sf/9 cells and its biological function was evaluated in mice. Eighty-one 8-week old BALB/cj female mice were randomly assigned to two groups, and injected intraperitoneally with either LPS (8 mug/g of body weight, n = 41) or LPS plus rbosCD14 (6.8 mug/g of body weight, n = 40). Survival rate at 24 h after injection for mice injected with either LPS or LPS plus rbosCD14 was 30 and 72\%, respectively (P {\textlangle} 0.01). At 48 h survival rate was 7 and 37\%, respectively (P {\textlangle} 0.01). To investigate the protective effect of rbosCD14 on experimentally induced mastitis in mice, two abdominal contralateral mammary glands of 7 lactating BALB/cj mice were injected through the teat canal with 10-20 colony-forming units (CFU) of Escherichia coli. One gland simultaneously received rbosCD14 (6 mug) and the other saline. At 24 h after challenge, glands that received rbosCD14 had less swelling and hemorrhaging, significantly lower bacterial counts (P {\textlangle} 0.05) and lower concentrations of TNF-\ensuremath{\alpha} (P {\textlangle} 0.05). Results indicate that rbosCD14 is biologically functional and reduces mortality in mice from endotoxin shock and severity of intramammary infection by E. coli.},
  author       = {Lee, Jai-Wei and Paape, Max and Zhao, Xin},
  issn         = {0928-4249},
  journal      = {VETERINARY RESEARCH},
  keyword      = {ENDOTOXIN,LPS,SEPTIC SHOCK,MAMMARY-GLAND,CYTOKINE PRODUCTION,CD14-DEFICIENT MICE,NECROSIS-FACTOR-ALPHA,GRAM-NEGATIVE BACTERIA,LIPOPOLYSACCHARIDE-BINDING-PROTEIN,TNF-alpha,Escherichia coli,mastitis,CD14,GROWTH},
  language     = {eng},
  number       = {3},
  pages        = {307--316},
  title        = {Recombinant bovine soluble CD14 reduces severity of experimental Escherichia coli mastitis in mice},
  url          = {http://dx.doi.org/10.1051/vetres:2003006},
  volume       = {34},
  year         = {2003},
}

Altmetric
View in Altmetric
Web of Science
Times cited: