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Characterization of bovine FAS-associated death domain gene

(2005) ANIMAL GENETICS. 36(1). p.63-66
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Abstract
The FAS-associated death domain (FADD) protein is an adapter/signaling molecule that has been shown to function in human cells to promote apoptosis and to inhibit NF-kappaB activation. Because of the critical role that apoptosis and NF-kappaB play in a variety of disease states, we mapped the bovine FADD gene, sequenced bovine FADD cDNA, and characterized its expression in endothelial cells (EC). Sequencing of bovine FADD revealed approximately 65 and 58% amino acid sequence identity to its human and murine homologues, respectively. Bovine FADD was mapped to chromosome 29 by radiation hybrid mapping. In addition, the functionality of bovine FADD was studied. Expression of a bovine FADD dominant-negative construct blocked bacterial lipopolysaccharide (LPS)- and TNF-alpha-induced apoptosis in bovine EC consistent with previous studies of human FADD. In contrast to human FADD, elevated expression of bovine FADD had no effect on LPS- or TNF-alpha-induced upregulation of NF-kappaB-dependent gene products as assayed by E-selectin expression. Thus, while the role of FADD in mediating apoptosis is conserved across species, its role in regulating NF-kappaB-dependent gene expression is not.
Keywords
caspase, apoptosis, cytokines, endotoxin, inflammation, NF-KAPPA-B, INDUCED APOPTOSIS, ESCHERICHIA-COLI, CD95 FAS/APO-1, TNF RECEPTOR, FADD, PROTEIN, ACTIVATION, CELLS, FLICE

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Chicago
Szperka, ME, EE Connor, Max Paape, JL Williams, and Douglas Bannerman. 2005. “Characterization of Bovine FAS-associated Death Domain Gene.” Animal Genetics 36 (1): 63–66.
APA
Szperka, M., Connor, E., Paape, M., Williams, J., & Bannerman, D. (2005). Characterization of bovine FAS-associated death domain gene. ANIMAL GENETICS, 36(1), 63–66.
Vancouver
1.
Szperka M, Connor E, Paape M, Williams J, Bannerman D. Characterization of bovine FAS-associated death domain gene. ANIMAL GENETICS. 2005;36(1):63–6.
MLA
Szperka, ME et al. “Characterization of Bovine FAS-associated Death Domain Gene.” ANIMAL GENETICS 36.1 (2005): 63–66. Print.
@article{820585,
  abstract     = {The FAS-associated death domain (FADD) protein is an adapter/signaling molecule that has been shown to function in human cells to promote apoptosis and to inhibit NF-kappaB activation. Because of the critical role that apoptosis and NF-kappaB play in a variety of disease states, we mapped the bovine FADD gene, sequenced bovine FADD cDNA, and characterized its expression in endothelial cells (EC). Sequencing of bovine FADD revealed approximately 65 and 58% amino acid sequence identity to its human and murine homologues, respectively. Bovine FADD was mapped to chromosome 29 by radiation hybrid mapping. In addition, the functionality of bovine FADD was studied. Expression of a bovine FADD dominant-negative construct blocked bacterial lipopolysaccharide (LPS)- and TNF-alpha-induced apoptosis in bovine EC consistent with previous studies of human FADD. In contrast to human FADD, elevated expression of bovine FADD had no effect on LPS- or TNF-alpha-induced upregulation of NF-kappaB-dependent gene products as assayed by E-selectin expression. Thus, while the role of FADD in mediating apoptosis is conserved across species, its role in regulating NF-kappaB-dependent gene expression is not.},
  author       = {Szperka, ME and Connor, EE and Paape, Max and Williams, JL and Bannerman, Douglas},
  issn         = {0268-9146},
  journal      = {ANIMAL GENETICS},
  keywords     = {caspase,apoptosis,cytokines,endotoxin,inflammation,NF-KAPPA-B,INDUCED APOPTOSIS,ESCHERICHIA-COLI,CD95 FAS/APO-1,TNF RECEPTOR,FADD,PROTEIN,ACTIVATION,CELLS,FLICE},
  language     = {eng},
  number       = {1},
  pages        = {63--66},
  title        = {Characterization of bovine FAS-associated death domain gene},
  url          = {http://dx.doi.org/10.1111/j.1365-2052.2004.01207.x},
  volume       = {36},
  year         = {2005},
}

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