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Effect of a trivalent vaccine against Staphylococcus aureus mastitis lymphocyte subpopulations, antibody production, and neutrophil phagocytosis

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Abstract
The effect of a novel bovine mastitis trivalent vaccine, containing Staphylococcus aureus capsular polysaccharide type 5 (T5), 8 (T8), and 336 (T336), on lymphocyte subpopulations, antibody production, and neutrophil phagocytosis was evaluated. Twenty pregnant heifers were immunized with either the trivalent alone, trivalent emulsified in Freund's incomplete adjuvant (FICA), trivalent in aluminum hydroxide, or adjuvant only (FICA). Immunization was done 30 d before the expected calving date followed by 2 boosts in a 2-week interval. Compared to FICA, serum antigen-specific immunoglobulin (Ig)G(1) and IgG(2) were significantly increased in all the vaccinated groups before parturition and sustained until 3 wk postpartum. In comparison with the trivalent alone, formulation with either adjuvant enhanced production of IgG(2), but not IgG(1). Immune sera, which contained the highest amount of antibodies, slightly increased neutrophil phagocytosis to the 3 serotypes of killed S. aureus, but most of the differences were not significant due to large variation between the cows. The percentage of CD4+ lymphocyte was significantly higher in vaccinated groups than that of FICA 4 wk after the primary immunization. In comparison with FICA, cows inoculated with trivalent vaccine and adjuvants had an increased percentage of CD8+ lymphocytes at 2 time points, 2 wk before and after calving. Our results indicated that the whole cell trivalent vaccine, with or without adjuvants, is able to elicit antibody responses specific to the 3 capsular polysaccharide antigens. The increase of T8-specific IgG(2) was more noticeable when the vaccine was emulsified with adjuvants.
Keywords
ENCAPSULATION, MACROPHAGES, IMMUNOGLOBULIN, COWS, RESPONSES, ADJUVANTS, CONJUGATE, DAIRY HERDS, MAMMARY EPITHELIAL-CELLS, BOVINE-MILK

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Citation

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Chicago
Lee, Jai-Wei, Celia N O’Brien, Albert J Guidry, Max Paape, Kimberley A Shafer-Weaver, and Xin Zhao. 2005. “Effect of a Trivalent Vaccine Against Staphylococcus Aureus Mastitis Lymphocyte Subpopulations, Antibody Production, and Neutrophil Phagocytosis.” Canadian Journal of Veterinary Research-revue Canadienne De Recherche Veterinaire 69 (1): 11–18.
APA
Lee, J.-W., O’Brien, C. N., Guidry, A. J., Paape, M., Shafer-Weaver, K. A., & Zhao, X. (2005). Effect of a trivalent vaccine against Staphylococcus aureus mastitis lymphocyte subpopulations, antibody production, and neutrophil phagocytosis. CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE, 69(1), 11–18.
Vancouver
1.
Lee J-W, O’Brien CN, Guidry AJ, Paape M, Shafer-Weaver KA, Zhao X. Effect of a trivalent vaccine against Staphylococcus aureus mastitis lymphocyte subpopulations, antibody production, and neutrophil phagocytosis. CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE. 2005;69(1):11–8.
MLA
Lee, Jai-Wei et al. “Effect of a Trivalent Vaccine Against Staphylococcus Aureus Mastitis Lymphocyte Subpopulations, Antibody Production, and Neutrophil Phagocytosis.” CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE 69.1 (2005): 11–18. Print.
@article{820561,
  abstract     = {The effect of a novel bovine mastitis trivalent vaccine, containing Staphylococcus aureus capsular polysaccharide type 5 (T5), 8 (T8), and 336 (T336), on lymphocyte subpopulations, antibody production, and neutrophil phagocytosis was evaluated. Twenty pregnant heifers were immunized with either the trivalent alone, trivalent emulsified in Freund's incomplete adjuvant (FICA), trivalent in aluminum hydroxide, or adjuvant only (FICA). Immunization was done 30 d before the expected calving date followed by 2 boosts in a 2-week interval. Compared to FICA, serum antigen-specific immunoglobulin (Ig)G(1) and IgG(2) were significantly increased in all the vaccinated groups before parturition and sustained until 3 wk postpartum. In comparison with the trivalent alone, formulation with either adjuvant enhanced production of IgG(2), but not IgG(1). Immune sera, which contained the highest amount of antibodies, slightly increased neutrophil phagocytosis to the 3 serotypes of killed S. aureus, but most of the differences were not significant due to large variation between the cows. The percentage of CD4+ lymphocyte was significantly higher in vaccinated groups than that of FICA 4 wk after the primary immunization. In comparison with FICA, cows inoculated with trivalent vaccine and adjuvants had an increased percentage of CD8+ lymphocytes at 2 time points, 2 wk before and after calving. Our results indicated that the whole cell trivalent vaccine, with or without adjuvants, is able to elicit antibody responses specific to the 3 capsular polysaccharide antigens. The increase of T8-specific IgG(2) was more noticeable when the vaccine was emulsified with adjuvants.},
  author       = {Lee, Jai-Wei and O'Brien, Celia N and Guidry, Albert J and Paape, Max and Shafer-Weaver, Kimberley A and Zhao, Xin},
  issn         = {0830-9000},
  journal      = {CANADIAN JOURNAL OF VETERINARY RESEARCH-REVUE CANADIENNE DE RECHERCHE VETERINAIRE},
  keywords     = {ENCAPSULATION,MACROPHAGES,IMMUNOGLOBULIN,COWS,RESPONSES,ADJUVANTS,CONJUGATE,DAIRY HERDS,MAMMARY EPITHELIAL-CELLS,BOVINE-MILK},
  language     = {eng},
  number       = {1},
  pages        = {11--18},
  title        = {Effect of a trivalent vaccine against Staphylococcus aureus mastitis lymphocyte subpopulations, antibody production, and neutrophil phagocytosis},
  volume       = {69},
  year         = {2005},
}

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