Ghent University Academic Bibliography

Advanced

The resident microflora of voided midstream urine of healthy controls : standard versus expanded urine culture protocols

Liselotte Coorevits UGent, Stefan Heytens UGent, Jerina Boelens UGent and Geert Claeys UGent (2017) EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. 36(4). p.635-639
abstract
The workup and interpretation of urine cultures is not always clear-cut, especially for midstream samples contaminated with commensals. Standard urine culture (SUC) protocols are designed in favor of growth of uropathogens at the expense of commensals. In selected clinical situations, however, it is essential to trace fastidious or new uropathogens by expanding the urine culture conditions (EUC). The aim of our study was to map the microflora in midstream urine specimens from healthy controls by means of EUC, in view of the interpretation of bacterial culture results in symptomatic patients. Midstream urine specimens from 101 healthy controls (86 females and 15 males) were examined using both SUC and EUC. Whilst 73 % of samples examined by SUC showed no growth at 103 colony-forming units (CFU)/mL, 91 % of samples examined by EUC grew bacterial species in large numbers (≥104 CFU/mL). Asymptomatic bacteriuria, as defined by the European guidelines for urinalysis, was detected in six samples with both protocols. EUC revealed 98 different species, mostly Lactobacillus, Staphylococcus, Streptococcus, and Corynebacterium. None of the samples grew Staphylococcus saprophyticus, Corynebacterium urealyticum, or Aerococcus urinae. Samples from females contained higher bacterial loads and showed higher bacterial diversity compared to males. Midstream urine of healthy controls contains large communities of living bacteria that comprise a resident microflora, only revealed by EUC. Hence, the use of EUC instead of SUC in a routine setting would result in more sensitive but less specific results, requiring critical interpretation. In our view, EUC should be reserved for limited indications.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
urinary tract infection, resident flora, urinary microflora, expanded urine culture, ADULT FEMALE BLADDER, 16S RDNA, TRACT, BACTERIURIA, DIAGNOSIS
journal title
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
Eur. J. Clin. Microbiol. Infect. Dis.
volume
36
issue
4
pages
635 - 639
Web of Science type
Article
Web of Science id
000398838200006
ISSN
0934-9723
DOI
10.1007/s10096-016-2839-x
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8203716
handle
http://hdl.handle.net/1854/LU-8203716
date created
2016-12-04 23:48:51
date last changed
2017-05-11 09:01:17
@article{8203716,
  abstract     = {The workup and interpretation of urine cultures is not always clear-cut, especially for midstream samples contaminated with commensals. Standard urine culture (SUC) protocols are designed in favor of growth of uropathogens at the expense of commensals. In selected clinical situations, however, it is essential to trace fastidious or new uropathogens by expanding the urine culture conditions (EUC). The aim of our study was to map the microflora in midstream urine specimens from healthy controls by means of EUC, in view of the interpretation of bacterial culture results in symptomatic patients. Midstream urine specimens from 101 healthy controls (86 females and 15 males) were examined using both SUC and EUC. Whilst 73 \% of samples examined by SUC showed no growth at 103 colony-forming units (CFU)/mL, 91 \% of samples examined by EUC grew bacterial species in large numbers (\ensuremath{\geq}104 CFU/mL). Asymptomatic bacteriuria, as defined by the European guidelines for urinalysis, was detected in six samples with both protocols. EUC revealed 98 different species, mostly Lactobacillus, Staphylococcus, Streptococcus, and Corynebacterium. None of the samples grew Staphylococcus saprophyticus, Corynebacterium urealyticum, or Aerococcus urinae. Samples from females contained higher bacterial loads and showed higher bacterial diversity compared to males. Midstream urine of healthy controls contains large communities of living bacteria that comprise a resident microflora, only revealed by EUC. Hence, the use of EUC instead of SUC in a routine setting would result in more sensitive but less specific results, requiring critical interpretation. In our view, EUC should be reserved for limited indications.},
  author       = {Coorevits, Liselotte and Heytens, Stefan and Boelens, Jerina and Claeys, Geert},
  issn         = {0934-9723},
  journal      = {EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY \& INFECTIOUS DISEASES},
  keyword      = {urinary tract infection,resident flora,urinary microflora,expanded urine culture,ADULT FEMALE BLADDER,16S RDNA,TRACT,BACTERIURIA,DIAGNOSIS},
  language     = {eng},
  number       = {4},
  pages        = {635--639},
  title        = {The resident microflora of voided midstream urine of healthy controls : standard versus expanded urine culture protocols},
  url          = {http://dx.doi.org/10.1007/s10096-016-2839-x},
  volume       = {36},
  year         = {2017},
}

Chicago
Coorevits, Liselotte, Stefan Heytens, Jerina Boelens, and Geert Claeys. 2017. “The Resident Microflora of Voided Midstream Urine of Healthy Controls : Standard Versus Expanded Urine Culture Protocols.” European Journal of Clinical Microbiology & Infectious Diseases 36 (4): 635–639.
APA
Coorevits, Liselotte, Heytens, S., Boelens, J., & Claeys, G. (2017). The resident microflora of voided midstream urine of healthy controls : standard versus expanded urine culture protocols. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 36(4), 635–639.
Vancouver
1.
Coorevits L, Heytens S, Boelens J, Claeys G. The resident microflora of voided midstream urine of healthy controls : standard versus expanded urine culture protocols. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES. 2017;36(4):635–9.
MLA
Coorevits, Liselotte, Stefan Heytens, Jerina Boelens, et al. “The Resident Microflora of Voided Midstream Urine of Healthy Controls : Standard Versus Expanded Urine Culture Protocols.” EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES 36.4 (2017): 635–639. Print.