Advanced search
1 file | 2.30 MB Add to list

Redirection of auxin flow in Arabidopsis thaliana roots after infection by root-knot nematodes

(2016) JOURNAL OF EXPERIMENTAL BOTANY. 67(15). p.4559-4570
Author
Organization
Abstract
Plant auxin efflux and influx proteins redirect the plant hormone auxin towards the feeding site upon root-knot nematode infection in Arabidopsis thaliana roots.Plant-parasitic root-knot nematodes induce the formation of giant cells within the plant root, and it has been recognized that auxin accumulates in these feeding sites. Here, we studied the role of the auxin transport system governed by AUX1/LAX3 influx proteins and different PIN efflux proteins during feeding site development in Arabidopsis thaliana roots. Data generated via promoter-reporter line and protein localization analyses evoke a model in which auxin is being imported at the basipetal side of the feeding site by the concerted action of the influx proteins AUX1 and LAX3, and the efflux protein PIN3. Mutants in auxin influx proteins AUX1 and LAX3 bear significantly fewer and smaller galls, revealing that auxin import into the feeding sites is needed for their development and expansion. The feeding site development in auxin export (PIN) mutants was only slightly hampered. Expression of some PINs appears to be suppressed in galls, probably to prevent auxin drainage. Nevertheless, a functional PIN4 gene seems to be a prerequisite for proper nematode development and gall expansion, most likely by removing excessive auxin to stabilize the hormone level in the feeding site. Our data also indicate a role of local auxin peaks in nematode attraction towards the root.
Keywords
FEEDING SITES, PLANT-PARASITIC NEMATODES, DIRECTS AUXIN, EFFLUX, INDUCTION, GRADIENTS, ETHYLENE, CYST, LOCALIZATION, GRAVITROPISM, root-knot nematode, PIN, LAX3, galls, auxin, AUX1, Arabidopsis

Downloads

  • 2016-Kyndt-jxb erw230-1.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 2.30 MB

Citation

Please use this url to cite or link to this publication:

MLA
Kyndt, Tina et al. “Redirection of Auxin Flow in Arabidopsis Thaliana Roots After Infection by Root-knot Nematodes.” JOURNAL OF EXPERIMENTAL BOTANY 67.15 (2016): 4559–4570. Print.
APA
Kyndt, T., Goverse, A., Haegeman, A., Warmerdam, S., Wanjau, C. N., Jahani, M., Engler, G., et al. (2016). Redirection of auxin flow in Arabidopsis thaliana roots after infection by root-knot nematodes. JOURNAL OF EXPERIMENTAL BOTANY, 67(15), 4559–4570.
Chicago author-date
Kyndt, Tina, Aska Goverse, Annelies Haegeman, Sonja Warmerdam, Cecilia Ngonyo Wanjau, Mona Jahani, Gilbert Engler, Janice de Almeida Engler, and Godelieve Gheysen. 2016. “Redirection of Auxin Flow in Arabidopsis Thaliana Roots After Infection by Root-knot Nematodes.” Journal of Experimental Botany 67 (15): 4559–4570.
Chicago author-date (all authors)
Kyndt, Tina, Aska Goverse, Annelies Haegeman, Sonja Warmerdam, Cecilia Ngonyo Wanjau, Mona Jahani, Gilbert Engler, Janice de Almeida Engler, and Godelieve Gheysen. 2016. “Redirection of Auxin Flow in Arabidopsis Thaliana Roots After Infection by Root-knot Nematodes.” Journal of Experimental Botany 67 (15): 4559–4570.
Vancouver
1.
Kyndt T, Goverse A, Haegeman A, Warmerdam S, Wanjau CN, Jahani M, et al. Redirection of auxin flow in Arabidopsis thaliana roots after infection by root-knot nematodes. JOURNAL OF EXPERIMENTAL BOTANY. 2016;67(15):4559–70.
IEEE
[1]
T. Kyndt et al., “Redirection of auxin flow in Arabidopsis thaliana roots after infection by root-knot nematodes,” JOURNAL OF EXPERIMENTAL BOTANY, vol. 67, no. 15, pp. 4559–4570, 2016.
@article{8203277,
  abstract     = {Plant auxin efflux and influx proteins redirect the plant hormone auxin towards the feeding site upon root-knot nematode infection in Arabidopsis thaliana roots.Plant-parasitic root-knot nematodes induce the formation of giant cells within the plant root, and it has been recognized that auxin accumulates in these feeding sites. Here, we studied the role of the auxin transport system governed by AUX1/LAX3 influx proteins and different PIN efflux proteins during feeding site development in Arabidopsis thaliana roots. Data generated via promoter-reporter line and protein localization analyses evoke a model in which auxin is being imported at the basipetal side of the feeding site by the concerted action of the influx proteins AUX1 and LAX3, and the efflux protein PIN3. Mutants in auxin influx proteins AUX1 and LAX3 bear significantly fewer and smaller galls, revealing that auxin import into the feeding sites is needed for their development and expansion. The feeding site development in auxin export (PIN) mutants was only slightly hampered. Expression of some PINs appears to be suppressed in galls, probably to prevent auxin drainage. Nevertheless, a functional PIN4 gene seems to be a prerequisite for proper nematode development and gall expansion, most likely by removing excessive auxin to stabilize the hormone level in the feeding site. Our data also indicate a role of local auxin peaks in nematode attraction towards the root.},
  author       = {Kyndt, Tina and Goverse, Aska and Haegeman, Annelies and Warmerdam, Sonja and Wanjau, Cecilia Ngonyo and Jahani, Mona and Engler, Gilbert and de Almeida Engler, Janice and Gheysen, Godelieve},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keywords     = {FEEDING SITES,PLANT-PARASITIC NEMATODES,DIRECTS AUXIN,EFFLUX,INDUCTION,GRADIENTS,ETHYLENE,CYST,LOCALIZATION,GRAVITROPISM,root-knot nematode,PIN,LAX3,galls,auxin,AUX1,Arabidopsis},
  language     = {eng},
  number       = {15},
  pages        = {4559--4570},
  title        = {Redirection of auxin flow in Arabidopsis thaliana roots after infection by root-knot nematodes},
  url          = {http://dx.doi.org/10.1093/jxb/erw230},
  volume       = {67},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: