Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles
- Author
- Timothy Douglas (UGent) , Agata Łapa, Katarzyna Reczyńska, Małgorzata Krok-Borkowicz, Krzysztof Pietryga, Sangram Keshari Samal (UGent) , Heidi Declercq (UGent) , David Schaubroeck (UGent) , Marijn Boone (UGent) , Pascal Van Der Voort (UGent) , Karel De Schamphelaere (UGent) , Christian Stevens (UGent) , Vitaliy Bliznuk (UGent) , Lieve Balcaen (UGent) , Bogdan Parakhonskiy (UGent) , Frank Vanhaecke (UGent) , Veerle Cnudde (UGent) , Elżbieta Pamuła and Andre Skirtach (UGent)
- Organization
-
- Department of Inorganic and physical chemistry (ceased 1-1-2018)
- Department of Geology
- Department of Electronics and information systems
- Department of Basic Medical Sciences (ceased 1-10-2018)
- Department of Information technology
- Laboratories of the Faculty of Pharmaceutical Sciences
- Department of Analytical chemistry (ceased 1-1-2018)
- Department of Applied ecology and environmental biology (ceased 1-1-2018)
- Department of Sustainable Organic Chemistry and Technology (ceased 1-1-2018)
- Department of Materials Science and Engineering (ceased 1-2-2017)
- Department of Molecular biotechnology (ceased 1-1-2018)
- Imec
- Department of Materials, Textiles and Chemical Engineering
- Project
- Abstract
- The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability. In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites. It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation. The effect of Mg incorporation on cytocompatibility and cell growth was also studied. Microparticles were formed by mixing Ca2+ and Mg2+ and CO32- ions in varying concentrations. Microparticles were characterized physiochemically and subsequently mixed with GG solution to form hydrogel-microparticle composites. The elemental Ca: Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added. In the absence of Mg, vaterite was formed. At low Mg content, magnesian calcite was formed. Increasing the Mg content further caused formation of amorphous mineral. Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min. Microparticles were dispersed homogeneously in hydrogels. MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves. All composites were cytocompatible. Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio. In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
- Keywords
- hydrogel, magnesium, gellan gum, injectable material, carbonate, composite, CELL-LINES, VATERITE PARTICLES, ENCAPSULATION, OSTEOBLASTS, DOLOMITE, MINERALS, DELIVERY, BEHAVIOR, RELEASE, SEARCH
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8202590
- MLA
- Douglas, Timothy, et al. “Novel Injectable, Self-Gelling Hydrogel-Microparticle Composites for Bone Regeneration Consisting of Gellan Gum and Calcium and Magnesium Carbonate Microparticles.” BIOMEDICAL MATERIALS, vol. 11, no. 6, 2016, doi:10.1088/1748-6041/11/6/065011.
- APA
- Douglas, T., Łapa, A., Reczyńska, K., Krok-Borkowicz, M., Pietryga, K., Samal, S. K., … Skirtach, A. (2016). Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles. BIOMEDICAL MATERIALS, 11(6). https://doi.org/10.1088/1748-6041/11/6/065011
- Chicago author-date
- Douglas, Timothy, Agata Łapa, Katarzyna Reczyńska, Małgorzata Krok-Borkowicz, Krzysztof Pietryga, Sangram Keshari Samal, Heidi Declercq, et al. 2016. “Novel Injectable, Self-Gelling Hydrogel-Microparticle Composites for Bone Regeneration Consisting of Gellan Gum and Calcium and Magnesium Carbonate Microparticles.” BIOMEDICAL MATERIALS 11 (6). https://doi.org/10.1088/1748-6041/11/6/065011.
- Chicago author-date (all authors)
- Douglas, Timothy, Agata Łapa, Katarzyna Reczyńska, Małgorzata Krok-Borkowicz, Krzysztof Pietryga, Sangram Keshari Samal, Heidi Declercq, David Schaubroeck, Marijn Boone, Pascal Van Der Voort, Karel De Schamphelaere, Christian Stevens, Vitaliy Bliznuk, Lieve Balcaen, Bogdan Parakhonskiy, Frank Vanhaecke, Veerle Cnudde, Elżbieta Pamuła, and Andre Skirtach. 2016. “Novel Injectable, Self-Gelling Hydrogel-Microparticle Composites for Bone Regeneration Consisting of Gellan Gum and Calcium and Magnesium Carbonate Microparticles.” BIOMEDICAL MATERIALS 11 (6). doi:10.1088/1748-6041/11/6/065011.
- Vancouver
- 1.Douglas T, Łapa A, Reczyńska K, Krok-Borkowicz M, Pietryga K, Samal SK, et al. Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles. BIOMEDICAL MATERIALS. 2016;11(6).
- IEEE
- [1]T. Douglas et al., “Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles,” BIOMEDICAL MATERIALS, vol. 11, no. 6, 2016.
@article{8202590, abstract = {{The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability. In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites. It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation. The effect of Mg incorporation on cytocompatibility and cell growth was also studied. Microparticles were formed by mixing Ca2+ and Mg2+ and CO32- ions in varying concentrations. Microparticles were characterized physiochemically and subsequently mixed with GG solution to form hydrogel-microparticle composites. The elemental Ca: Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added. In the absence of Mg, vaterite was formed. At low Mg content, magnesian calcite was formed. Increasing the Mg content further caused formation of amorphous mineral. Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min. Microparticles were dispersed homogeneously in hydrogels. MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves. All composites were cytocompatible. Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio. In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.}}, articleno = {{065011}}, author = {{Douglas, Timothy and Łapa, Agata and Reczyńska, Katarzyna and Krok-Borkowicz, Małgorzata and Pietryga, Krzysztof and Samal, Sangram Keshari and Declercq, Heidi and Schaubroeck, David and Boone, Marijn and Van Der Voort, Pascal and De Schamphelaere, Karel and Stevens, Christian and Bliznuk, Vitaliy and Balcaen, Lieve and Parakhonskiy, Bogdan and Vanhaecke, Frank and Cnudde, Veerle and Pamuła, Elżbieta and Skirtach, Andre}}, issn = {{1748-6041}}, journal = {{BIOMEDICAL MATERIALS}}, keywords = {{hydrogel,magnesium,gellan gum,injectable material,carbonate,composite,CELL-LINES,VATERITE PARTICLES,ENCAPSULATION,OSTEOBLASTS,DOLOMITE,MINERALS,DELIVERY,BEHAVIOR,RELEASE,SEARCH}}, language = {{eng}}, number = {{6}}, pages = {{13}}, title = {{Novel injectable, self-gelling hydrogel-microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles}}, url = {{http://doi.org/10.1088/1748-6041/11/6/065011}}, volume = {{11}}, year = {{2016}}, }
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