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Novel injectable, self-gelling hydrogel–microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles

Timothy Douglas, Agata Łapa, Katarzyna Reczyńska, Małgorzata Krok-Borkowicz, Krzysztof Pietryga, Sangram Keshari Samal, Heidi Declercq UGent, David Schaubroeck UGent, Marijn Boone, Pascal Van Der Voort UGent, et al. (2016) BIOMEDICAL MATERIALS. 11(6).
abstract
The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability. In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites. It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation. The effect of Mg incorporation on cytocompatibility and cell growth was also studied. Microparticles were formed by mixing Ca2+ and Mg2+ and CO32- ions in varying concentrations. Microparticles were characterized physiochemically and subsequently mixed with GG solution to form hydrogel-microparticle composites. The elemental Ca: Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added. In the absence of Mg, vaterite was formed. At low Mg content, magnesian calcite was formed. Increasing the Mg content further caused formation of amorphous mineral. Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min. Microparticles were dispersed homogeneously in hydrogels. MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves. All composites were cytocompatible. Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio. In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
hydrogel, magnesium, gellan gum, injectable material, carbonate, composite, CELL-LINES, VATERITE PARTICLES, ENCAPSULATION, OSTEOBLASTS, DOLOMITE, MINERALS, DELIVERY, BEHAVIOR, RELEASE, SEARCH
journal title
BIOMEDICAL MATERIALS
Biomed. Mater.
volume
11
issue
6
article number
065011
pages
13 pages
Web of Science type
Article
Web of Science id
000389330600002
JCR category
ENGINEERING, BIOMEDICAL
JCR impact factor
2.469 (2016)
JCR rank
27/77 (2016)
JCR quartile
2 (2016)
ISSN
1748-6041
DOI
10.1088/1748-6041/11/6/065011
project
Center for nano- and biophotonics (NB-Photonics)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8202590
handle
http://hdl.handle.net/1854/LU-8202590
date created
2016-12-02 14:18:55
date last changed
2018-01-29 12:12:43
@article{8202590,
  abstract     = {The suitability of hydrogel biomaterials for bone regeneration can be improved by incorporation of an inorganic phase in particle form, thus maintaining hydrogel injectability. In this study, carbonate microparticles containing different amounts of calcium (Ca) and magnesium (Mg) were added to solutions of the anionic polysaccharide gellan gum (GG) to crosslink GG by release of Ca2+ and Mg2+ from microparticles and thereby induce formation of hydrogel-microparticle composites. It was hypothesized that increasing Mg content of microparticles would promote GG hydrogel formation. The effect of Mg incorporation on cytocompatibility and cell growth was also studied. Microparticles were formed by mixing Ca2+ and Mg2+ and CO32- ions in varying concentrations. Microparticles were characterized physiochemically and subsequently mixed with GG solution to form hydrogel-microparticle composites. The elemental Ca: Mg ratio in the mineral formed was similar to the Ca:Mg ratio of the ions added. In the absence of Mg, vaterite was formed. At low Mg content, magnesian calcite was formed. Increasing the Mg content further caused formation of amorphous mineral. Microparticles of vaterite and magnesium calcite did not induce GG hydrogel formation, but addition of Mg-richer amorphous microparticles induced gelation within 20 min. Microparticles were dispersed homogeneously in hydrogels. MG-63 osteoblast-like cells were cultured in eluate from hydrogel-microparticle composites and on the composites themselves. All composites were cytocompatible. Cell growth was highest on composites containing particles with an equimolar Ca:Mg ratio. In summary, carbonate microparticles containing a sufficient amount of Mg induced GG hydrogel formation, resulting in injectable, cytocompatible hydrogel-microparticle composites.},
  articleno    = {065011},
  author       = {Douglas, Timothy and \unmatched{0141}apa, Agata and Reczy\'{n}ska, Katarzyna and Krok-Borkowicz, Ma\unmatched{0142}gorzata and Pietryga, Krzysztof and Samal, Sangram Keshari and Declercq, Heidi and Schaubroeck, David and Boone, Marijn and Van Der Voort, Pascal and De Schamphelaere, Karel and Stevens, Christian and Bliznuk, Vitaliy and Balcaen, Lieve and Parakhonskiy, Bogdan and Vanhaecke, Frank and Cnudde, Veerle and Pamu\unmatched{0142}a, El\.{z}bieta and Skirtach, Andre},
  issn         = {1748-6041},
  journal      = {BIOMEDICAL MATERIALS},
  keyword      = {hydrogel,magnesium,gellan gum,injectable material,carbonate,composite,CELL-LINES,VATERITE PARTICLES,ENCAPSULATION,OSTEOBLASTS,DOLOMITE,MINERALS,DELIVERY,BEHAVIOR,RELEASE,SEARCH},
  language     = {eng},
  number       = {6},
  pages        = {13},
  title        = {Novel injectable, self-gelling hydrogel--microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles},
  url          = {http://dx.doi.org/10.1088/1748-6041/11/6/065011},
  volume       = {11},
  year         = {2016},
}

Chicago
Douglas, Timothy, Agata Łapa, Katarzyna Reczyńska, Małgorzata Krok-Borkowicz, Krzysztof Pietryga, Sangram Keshari Samal, Heidi Declercq, et al. 2016. “Novel Injectable, Self-gelling Hydrogel–microparticle Composites for Bone Regeneration Consisting of Gellan Gum and Calcium and Magnesium Carbonate Microparticles.” Biomedical Materials 11 (6).
APA
Douglas, Timothy, Łapa, A., Reczyńska, K., Krok-Borkowicz, M., Pietryga, K., Samal, S. K., Declercq, H., et al. (2016). Novel injectable, self-gelling hydrogel–microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles. BIOMEDICAL MATERIALS, 11(6).
Vancouver
1.
Douglas T, Łapa A, Reczyńska K, Krok-Borkowicz M, Pietryga K, Samal SK, et al. Novel injectable, self-gelling hydrogel–microparticle composites for bone regeneration consisting of gellan gum and calcium and magnesium carbonate microparticles. BIOMEDICAL MATERIALS. 2016;11(6).
MLA
Douglas, Timothy, Agata Łapa, Katarzyna Reczyńska, et al. “Novel Injectable, Self-gelling Hydrogel–microparticle Composites for Bone Regeneration Consisting of Gellan Gum and Calcium and Magnesium Carbonate Microparticles.” BIOMEDICAL MATERIALS 11.6 (2016): n. pag. Print.