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Cryopreservation of diatoms: tips and tricks

Eveline Pinseel (UGent) , Willem Stock (UGent) , Sam De Decker (UGent) , Lander Blommaert (UGent) , Josefin Sefbom (UGent) , Olga Chepurnova (UGent) and Wim Vyverman (UGent)
Author
Organization
Abstract
Diatoms are an ecological and evolutionary important group of microalgae and hold much value for aquaculture and overall biotechnological potential. In contrast to many other groups of micro-organisms, long-term maintenance of diatom strains by repeated re-inoculation is impossible due to the gradual reduction in cell size of most species, eventually resulting in cell death. Therefore, cryopreservation is the preferred strain preservation method. Cryopreservation success varies strongly with species and differs systematically between marine and freshwater diatoms. Based on previous experiences in the BCCM/DCG culture collection (Laboratory of Protistology and Aquatic Ecology, Ghent University), 80% of the tested marine species (n=50) could be cryopreserved successfully, while this was the case only for 25% of tested freshwater species (n=20). Here we formally compare different cryopreservation protocols tested for a panel of model freshwater and marine species of the genera Pinnularia, Seminavis, Cyclotella, Cylindrotheca, Thalasiosira and Opephora. We will discuss strategies to optimize cryopreservation procedures for diatoms and possible alternatives for the long-term maintenance of diatom species in culture collections.

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MLA
Pinseel, Eveline, Willem Stock, Sam De Decker, et al. “Cryopreservation of Diatoms: Tips and Tricks.” 24th International Diatom Symposium : Program and Abstracts. 2016. 216–216. Print.
APA
Pinseel, E., Stock, W., De Decker, S., Blommaert, L., Sefbom, J., Chepurnova, O., & Vyverman, W. (2016). Cryopreservation of diatoms: tips and tricks. 24th International diatom symposium : program and abstracts (pp. 216–216). Presented at the 24th International Diatom symposium.
Chicago author-date
Pinseel, Eveline, Willem Stock, Sam De Decker, Lander Blommaert, Josefin Sefbom, Olga Chepurnova, and Wim Vyverman. 2016. “Cryopreservation of Diatoms: Tips and Tricks.” In 24th International Diatom Symposium : Program and Abstracts, 216–216.
Chicago author-date (all authors)
Pinseel, Eveline, Willem Stock, Sam De Decker, Lander Blommaert, Josefin Sefbom, Olga Chepurnova, and Wim Vyverman. 2016. “Cryopreservation of Diatoms: Tips and Tricks.” In 24th International Diatom Symposium : Program and Abstracts, 216–216.
Vancouver
1.
Pinseel E, Stock W, De Decker S, Blommaert L, Sefbom J, Chepurnova O, et al. Cryopreservation of diatoms: tips and tricks. 24th International diatom symposium : program and abstracts. 2016. p. 216–216.
IEEE
[1]
E. Pinseel et al., “Cryopreservation of diatoms: tips and tricks,” in 24th International diatom symposium : program and abstracts, Québec, QB, Canada, 2016, pp. 216–216.
@inproceedings{8197099,
  abstract     = {Diatoms are an ecological and evolutionary important group of microalgae and hold much value for aquaculture and overall biotechnological potential. In contrast to many other groups of micro-organisms, long-term maintenance of diatom strains by repeated re-inoculation is impossible due to the gradual reduction in cell size of most species, eventually resulting in cell death. Therefore, cryopreservation is the preferred strain preservation method. Cryopreservation success varies strongly with species and differs systematically between marine and freshwater diatoms. Based on previous experiences in the BCCM/DCG culture collection (Laboratory of Protistology and Aquatic Ecology, Ghent University), 80% of the tested marine species (n=50) could be cryopreserved successfully, while this was the case only for 25% of tested freshwater species (n=20). Here we formally compare different cryopreservation protocols tested for a panel of model freshwater and marine species of the genera Pinnularia, Seminavis, Cyclotella, Cylindrotheca, Thalasiosira and Opephora. We will discuss strategies to optimize cryopreservation procedures for diatoms and possible alternatives for the long-term maintenance of diatom species in culture collections.},
  author       = {Pinseel, Eveline and Stock, Willem and De Decker, Sam and Blommaert, Lander and Sefbom, Josefin and Chepurnova, Olga and Vyverman, Wim},
  booktitle    = {24th International diatom symposium : program and abstracts},
  language     = {eng},
  location     = {Québec, QB, Canada},
  pages        = {216--216},
  title        = {Cryopreservation of diatoms: tips and tricks},
  year         = {2016},
}