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The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis

(2016) EMBO JOURNAL. 35(19). p.2068-2086
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Abstract
Upon DNA damage, cyclin-dependent kinases (CDKs) are typically inhibited to block cell division. In many organisms, however, it has been found that CDK activity is required for DNA repair, especially for homology-dependent repair (HR), resulting in the conundrum how mitotic arrest and repair can be reconciled. Here, we show that Arabidopsis thaliana solves this dilemma by a division of labor strategy. We identify the plant-specific B1-type CDKs (CDKB1s) and the class of B1-type cyclins (CYCB1s) as major regulators of HR in plants. We find that RADIATION SENSITIVE 51 (RAD51), a core mediator of HR, is a substrate of CDKB1-CYCB1 complexes. Conversely, mutants in CDKB1 and CYCB1 fail to recruit RAD51 to damaged DNA. CYCB1; 1 is specifically activated after DNA damage and we show that this activation is directly controlled by SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), a transcription factor that acts similarly to p53 in animals. Thus, while the major mitotic cell-cycle activity is blocked after DNA damage, CDKB1-CYCB1 complexes are specifically activated to mediate HR.
Keywords
GENOTOXIC STRESS, RAD51 PARALOGS, END RESECTION, DEPENDENT-KINASES, IONIZING-RADIATION, CELL-CYCLE CONTROL, DNA-DAMAGE RESPONSE, DOUBLE-STRAND BREAKS, homologous recombination, DNA damage, cyclin, cell cycle, CDK, MESSENGER-RNA, GAMMA-RAYS

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Chicago
Weimer, Annika, Sascha Biedermann, Hirofumi Harashima, Farshad Roodbarkelari, Naoki Takahashi, Julia Foreman, Yonsheng Guan, et al. 2016. “The Plant-specific CDKB1-CYCB1 Complex Mediates Homologous Recombination Repair in Arabidopsis.” Embo Journal 35 (19): 2068–2086.
APA
Weimer, A., Biedermann, S., Harashima, H., Roodbarkelari, F., Takahashi, N., Foreman, J., Guan, Y., et al. (2016). The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. EMBO JOURNAL, 35(19), 2068–2086.
Vancouver
1.
Weimer A, Biedermann S, Harashima H, Roodbarkelari F, Takahashi N, Foreman J, et al. The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. EMBO JOURNAL. 2016;35(19):2068–86.
MLA
Weimer, Annika, Sascha Biedermann, Hirofumi Harashima, et al. “The Plant-specific CDKB1-CYCB1 Complex Mediates Homologous Recombination Repair in Arabidopsis.” EMBO JOURNAL 35.19 (2016): 2068–2086. Print.
@article{8174727,
  abstract     = {Upon DNA damage, cyclin-dependent kinases (CDKs) are typically inhibited to block cell division. In many organisms, however, it has been found that CDK activity is required for DNA repair, especially for homology-dependent repair (HR), resulting in the conundrum how mitotic arrest and repair can be reconciled. Here, we show that Arabidopsis thaliana solves this dilemma by a division of labor strategy. We identify the plant-specific B1-type CDKs (CDKB1s) and the class of B1-type cyclins (CYCB1s) as major regulators of HR in plants. We find that RADIATION SENSITIVE 51 (RAD51), a core mediator of HR, is a substrate of CDKB1-CYCB1 complexes. Conversely, mutants in CDKB1 and CYCB1 fail to recruit RAD51 to damaged DNA. CYCB1; 1 is specifically activated after DNA damage and we show that this activation is directly controlled by SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), a transcription factor that acts similarly to p53 in animals. Thus, while the major mitotic cell-cycle activity is blocked after DNA damage, CDKB1-CYCB1 complexes are specifically activated to mediate HR.},
  author       = {Weimer, Annika and Biedermann, Sascha and Harashima, Hirofumi and Roodbarkelari, Farshad and Takahashi, Naoki and Foreman, Julia and Guan, Yonsheng and Pochon, Ga{\"e}tan and Heese, Maren and Van Damme, Dani{\"e}l and Sugimoto, Keiko and Koncz, Csaba and Doerner, Peter and Umeda, Masaaki and Schnittger, Arp},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keyword      = {GENOTOXIC STRESS,RAD51 PARALOGS,END RESECTION,DEPENDENT-KINASES,IONIZING-RADIATION,CELL-CYCLE CONTROL,DNA-DAMAGE RESPONSE,DOUBLE-STRAND BREAKS,homologous recombination,DNA damage,cyclin,cell cycle,CDK,MESSENGER-RNA,GAMMA-RAYS},
  language     = {eng},
  number       = {19},
  pages        = {2068--2086},
  title        = {The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis},
  url          = {http://dx.doi.org/10.15252/embj.201593083},
  volume       = {35},
  year         = {2016},
}

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