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The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis

Annika Weimer, Sascha Biedermann, Hirofumi Harashima, Farshad Roodbarkelari, Naoki Takahashi, Julia Foreman, Yonsheng Guan, Gaëtan Pochon, Maren Heese, Daniël Van Damme UGent, et al. (2016) EMBO JOURNAL. 35(19). p.2068-2086
abstract
Upon DNA damage, cyclin-dependent kinases (CDKs) are typically inhibited to block cell division. In many organisms, however, it has been found that CDK activity is required for DNA repair, especially for homology-dependent repair (HR), resulting in the conundrum how mitotic arrest and repair can be reconciled. Here, we show that Arabidopsis thaliana solves this dilemma by a division of labor strategy. We identify the plant-specific B1-type CDKs (CDKB1s) and the class of B1-type cyclins (CYCB1s) as major regulators of HR in plants. We find that RADIATION SENSITIVE 51 (RAD51), a core mediator of HR, is a substrate of CDKB1-CYCB1 complexes. Conversely, mutants in CDKB1 and CYCB1 fail to recruit RAD51 to damaged DNA. CYCB1; 1 is specifically activated after DNA damage and we show that this activation is directly controlled by SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), a transcription factor that acts similarly to p53 in animals. Thus, while the major mitotic cell-cycle activity is blocked after DNA damage, CDKB1-CYCB1 complexes are specifically activated to mediate HR.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
GENOTOXIC STRESS, RAD51 PARALOGS, END RESECTION, DEPENDENT-KINASES, IONIZING-RADIATION, CELL-CYCLE CONTROL, DNA-DAMAGE RESPONSE, DOUBLE-STRAND BREAKS, homologous recombination, DNA damage, cyclin, cell cycle, CDK, MESSENGER-RNA, GAMMA-RAYS
journal title
EMBO JOURNAL
Embo J.
volume
35
issue
19
pages
2068 - 2086
Web of Science type
Article
Web of Science id
000385707500004
JCR category
BIOCHEMISTRY & MOLECULAR BIOLOGY
JCR impact factor
9.792 (2016)
JCR rank
16/286 (2016)
JCR quartile
1 (2016)
ISSN
0261-4189
DOI
10.15252/embj.201593083
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8174727
handle
http://hdl.handle.net/1854/LU-8174727
date created
2016-11-28 13:27:54
date last changed
2017-03-02 09:51:57
@article{8174727,
  abstract     = {Upon DNA damage, cyclin-dependent kinases (CDKs) are typically inhibited to block cell division. In many organisms, however, it has been found that CDK activity is required for DNA repair, especially for homology-dependent repair (HR), resulting in the conundrum how mitotic arrest and repair can be reconciled. Here, we show that Arabidopsis thaliana solves this dilemma by a division of labor strategy. We identify the plant-specific B1-type CDKs (CDKB1s) and the class of B1-type cyclins (CYCB1s) as major regulators of HR in plants. We find that RADIATION SENSITIVE 51 (RAD51), a core mediator of HR, is a substrate of CDKB1-CYCB1 complexes. Conversely, mutants in CDKB1 and CYCB1 fail to recruit RAD51 to damaged DNA. CYCB1; 1 is specifically activated after DNA damage and we show that this activation is directly controlled by SUPPRESSOR OF GAMMA RESPONSE 1 (SOG1), a transcription factor that acts similarly to p53 in animals. Thus, while the major mitotic cell-cycle activity is blocked after DNA damage, CDKB1-CYCB1 complexes are specifically activated to mediate HR.},
  author       = {Weimer, Annika and Biedermann, Sascha and Harashima, Hirofumi and Roodbarkelari, Farshad and Takahashi, Naoki and Foreman, Julia and Guan, Yonsheng and Pochon, Ga{\"e}tan and Heese, Maren and Van Damme, Dani{\"e}l and Sugimoto, Keiko and Koncz, Csaba and Doerner, Peter and Umeda, Masaaki and Schnittger, Arp},
  issn         = {0261-4189},
  journal      = {EMBO JOURNAL},
  keyword      = {GENOTOXIC STRESS,RAD51 PARALOGS,END RESECTION,DEPENDENT-KINASES,IONIZING-RADIATION,CELL-CYCLE CONTROL,DNA-DAMAGE RESPONSE,DOUBLE-STRAND BREAKS,homologous recombination,DNA damage,cyclin,cell cycle,CDK,MESSENGER-RNA,GAMMA-RAYS},
  language     = {eng},
  number       = {19},
  pages        = {2068--2086},
  title        = {The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis},
  url          = {http://dx.doi.org/10.15252/embj.201593083},
  volume       = {35},
  year         = {2016},
}

Chicago
Weimer, Annika, Sascha Biedermann, Hirofumi Harashima, Farshad Roodbarkelari, Naoki Takahashi, Julia Foreman, Yonsheng Guan, et al. 2016. “The Plant-specific CDKB1-CYCB1 Complex Mediates Homologous Recombination Repair in Arabidopsis.” Embo Journal 35 (19): 2068–2086.
APA
Weimer, A., Biedermann, S., Harashima, H., Roodbarkelari, F., Takahashi, N., Foreman, J., Guan, Y., et al. (2016). The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. EMBO JOURNAL, 35(19), 2068–2086.
Vancouver
1.
Weimer A, Biedermann S, Harashima H, Roodbarkelari F, Takahashi N, Foreman J, et al. The plant-specific CDKB1-CYCB1 complex mediates homologous recombination repair in Arabidopsis. EMBO JOURNAL. 2016;35(19):2068–86.
MLA
Weimer, Annika, Sascha Biedermann, Hirofumi Harashima, et al. “The Plant-specific CDKB1-CYCB1 Complex Mediates Homologous Recombination Repair in Arabidopsis.” EMBO JOURNAL 35.19 (2016): 2068–2086. Print.