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Flagellin-mediated protection against intestinal Yersinia pseudotuberculosis infection does not require interleukin-22

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Abstract
Signaling through Toll-like receptors (TLRs), the main receptors in innate immunity, is essential for the defense of mucosal surfaces. It was previously shown that systemic TLR5 stimulation by bacterial flagellin induces an immediate, transient interleukin-22 (IL-22)-dependent antimicrobial response to bacterial or viral infections of the mucosa. This process was dependent on the activation of type 3 innate lymphoid cells (ILCs). The objective of the present study was to analyze the effects of flagellin treatment in a murine model of oral infection with Yersinia pseudotuberculosis (an invasive, Gram-negative, enteropathogenic bacterium that targets the small intestine). We found that systemic administration of flagellin significantly increased the survival rate after intestinal infection (but not systemic infection) by Y. pseudotuberculosis. This protection was associated with a low bacterial count in the gut and the spleen. In contrast, no protection was afforded by administration of the TLR4 agonist lipopolysaccharide, suggesting the presence of a flagellin-specific effect. Lastly, we found that TLR5-and MyD88-mediated signaling was required for the protective effects of flagellin, whereas neither lymphoid cells nor IL-22 was involved.
Keywords
interleukin-22, TLR5, Yersinia pseudotuberculosis, flagellin, intestine, mouse infection, Toll-like receptors, INNATE LYMPHOID-CELLS, III SECRETION APPARATUS, BACTERIAL FLAGELLIN, ANTIMICROBIAL PEPTIDE, PNEUMONIAE INFECTION, COMMENSAL BACTERIA, NLRC4 INFLAMMASOME, IMMUNE-RESPONSES, EPITHELIAL-CELLS, DENDRITIC CELLS

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Chicago
Porte, Rémi, Laurye Van Maele, Natalia Muñoz-Wolf, Benoit Foligné, Laure Dumoutier, Julien Tabareau, Delphine Cayet, et al. 2017. “Flagellin-mediated Protection Against Intestinal Yersinia Pseudotuberculosis Infection Does Not Require Interleukin-22.” Infection and Immunity 85 (2).
APA
Porte, R., Van Maele, L., Muñoz-Wolf, N., Foligné, B., Dumoutier, L., Tabareau, J., Cayet, D., et al. (2017). Flagellin-mediated protection against intestinal Yersinia pseudotuberculosis infection does not require interleukin-22. INFECTION AND IMMUNITY, 85(2).
Vancouver
1.
Porte R, Van Maele L, Muñoz-Wolf N, Foligné B, Dumoutier L, Tabareau J, et al. Flagellin-mediated protection against intestinal Yersinia pseudotuberculosis infection does not require interleukin-22. INFECTION AND IMMUNITY. 2017;85(2).
MLA
Porte, Rémi, Laurye Van Maele, Natalia Muñoz-Wolf, et al. “Flagellin-mediated Protection Against Intestinal Yersinia Pseudotuberculosis Infection Does Not Require Interleukin-22.” INFECTION AND IMMUNITY 85.2 (2017): n. pag. Print.
@article{8174625,
  abstract     = {Signaling through Toll-like receptors (TLRs), the main receptors in innate immunity, is essential for the defense of mucosal surfaces. It was previously shown that systemic TLR5 stimulation by bacterial flagellin induces an immediate, transient interleukin-22 (IL-22)-dependent antimicrobial response to bacterial or viral infections of the mucosa. This process was dependent on the activation of type 3 innate lymphoid cells (ILCs). The objective of the present study was to analyze the effects of flagellin treatment in a murine model of oral infection with Yersinia pseudotuberculosis (an invasive, Gram-negative, enteropathogenic bacterium that targets the small intestine). We found that systemic administration of flagellin significantly increased the survival rate after intestinal infection (but not systemic infection) by Y. pseudotuberculosis. This protection was associated with a low bacterial count in the gut and the spleen. In contrast, no protection was afforded by administration of the TLR4 agonist lipopolysaccharide, suggesting the presence of a flagellin-specific effect. Lastly, we found that TLR5-and MyD88-mediated signaling was required for the protective effects of flagellin, whereas neither lymphoid cells nor IL-22 was involved.},
  articleno    = {e00806-16},
  author       = {Porte, R{\'e}mi and Van Maele, Laurye and Mu{\~n}oz-Wolf, Natalia and Folign{\'e}, Benoit and Dumoutier, Laure and Tabareau, Julien and Cayet, Delphine and Gosset, Pierre and Jonckheere, Nicolas and Van Seuningen, Isabelle and Chabalgoity, A Jos{\'e} and Simonet, Michel and Lamkanfi, Mohamed and Renauld, Jean-Christophe and Sirard, Jean-Claude and Carnoy, Christophe},
  issn         = {0019-9567},
  journal      = {INFECTION AND IMMUNITY},
  keyword      = {interleukin-22,TLR5,Yersinia pseudotuberculosis,flagellin,intestine,mouse infection,Toll-like receptors,INNATE LYMPHOID-CELLS,III SECRETION APPARATUS,BACTERIAL FLAGELLIN,ANTIMICROBIAL PEPTIDE,PNEUMONIAE INFECTION,COMMENSAL BACTERIA,NLRC4 INFLAMMASOME,IMMUNE-RESPONSES,EPITHELIAL-CELLS,DENDRITIC CELLS},
  language     = {eng},
  number       = {2},
  pages        = {12},
  title        = {Flagellin-mediated protection against intestinal Yersinia pseudotuberculosis infection does not require interleukin-22},
  url          = {http://dx.doi.org/10.1128/IAI.00806-16},
  volume       = {85},
  year         = {2017},
}

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