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Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability

Ranhua Xiong (UGent) , Roosmarijn Vandenbroucke (UGent) , Katleen Broos, Toon Brans (UGent) , Elien Van Wonterghem (UGent) , Claude Libert (UGent) , Jo Demeester (UGent) , Stefaan De Smedt (UGent) and Kevin Braeckmans (UGent)
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Center for nano- and biophotonics (NB-Photonics)
Abstract
Sizing nanomaterials in complex biological fluids, such as blood, remains a great challenge in spite of its importance for a wide range of biomedical applications. In drug delivery, for instance, it is essential that aggregation of protein-based drugs is avoided as it may alter their efficacy or elicit immune responses. Similarly it is of interest to determine which size of molecules can pass through biological barriers in vivo to diagnose pathologies, such as sepsis. Here, we report on continuous fluorescence recovery after photobleaching (cFRAP) as a analytical method enabling size distribution measurements of nanomaterials (1-100 nm) in undiluted biological fluids. We demonstrate that cFRAP allows to measure protein aggregation in human serum and to determine the permeability of intestinal and vascular barriers in vivo. cFRAP is a new analytical technique that paves the way towards exciting new applications that benefit from nanomaterial sizing in bio-fluids.
Keywords
SINGLE-PARTICLE TRACKING, INTESTINAL PERMEABILITY, LASER-SCANNING MICROSCOPE, FLUORESCENCE RECOVERY, DRUG-DELIVERY, SIZE, NANOPARTICLES, DIFFUSION, EFFICIENCY, THERAPY

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Citation

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Chicago
Xiong, Ranhua, Roosmarijn Vandenbroucke, Katleen Broos, Toon Brans, Elien Van Wonterghem, Claude Libert, Jo Demeester, Stefaan De Smedt, and Kevin Braeckmans. 2016. “Sizing Nanomaterials in Bio-fluids by cFRAP Enables Protein Aggregation Measurements and Diagnosis of Bio-barrier Permeability.” Nature Communications 7.
APA
Xiong, R., Vandenbroucke, R., Broos, K., Brans, T., Van Wonterghem, E., Libert, C., Demeester, J., et al. (2016). Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability. NATURE COMMUNICATIONS, 7.
Vancouver
1.
Xiong R, Vandenbroucke R, Broos K, Brans T, Van Wonterghem E, Libert C, et al. Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability. NATURE COMMUNICATIONS. 2016;7.
MLA
Xiong, Ranhua, Roosmarijn Vandenbroucke, Katleen Broos, et al. “Sizing Nanomaterials in Bio-fluids by cFRAP Enables Protein Aggregation Measurements and Diagnosis of Bio-barrier Permeability.” NATURE COMMUNICATIONS 7 (2016): n. pag. Print.
@article{8167381,
  abstract     = {Sizing nanomaterials in complex biological fluids, such as blood, remains a great challenge in spite of its importance for a wide range of biomedical applications. In drug delivery, for instance, it is essential that aggregation of protein-based drugs is avoided as it may alter their efficacy or elicit immune responses. Similarly it is of interest to determine which size of molecules can pass through biological barriers in vivo to diagnose pathologies, such as sepsis. Here, we report on continuous fluorescence recovery after photobleaching (cFRAP) as a analytical method enabling size distribution measurements of nanomaterials (1-100 nm) in undiluted biological fluids. We demonstrate that cFRAP allows to measure protein aggregation in human serum and to determine the permeability of intestinal and vascular barriers in vivo. cFRAP is a new analytical technique that paves the way towards exciting new applications that benefit from nanomaterial sizing in bio-fluids.},
  articleno    = {12982},
  author       = {Xiong, Ranhua and Vandenbroucke, Roosmarijn and Broos, Katleen and Brans, Toon and Van Wonterghem, Elien and Libert, Claude and Demeester, Jo and De Smedt, Stefaan and Braeckmans, Kevin},
  issn         = {2041-1723},
  journal      = {NATURE COMMUNICATIONS},
  language     = {eng},
  pages        = {10},
  title        = {Sizing nanomaterials in bio-fluids by cFRAP enables protein aggregation measurements and diagnosis of bio-barrier permeability},
  url          = {http://dx.doi.org/10.1038/ncomms12982},
  volume       = {7},
  year         = {2016},
}

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