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Anti-viral effect of Bifidobacterium adolescentis against noroviruses

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Abstract
This study aims to investigate the effect of Bifidobacterium adolescentis against noroviruses (NoVs). Murine norovirus-1 (MNV-1) used as a surrogate was detected by plaque assay and RT-qPCR. Human NoV virus like particles (VLPs) were detected by cell-binding assay. It was shown that the presence of B. adolescentis could inhibit the multiplication of MNV-1 on RAW 264.7 cells within 48 h of co-incubation period at 37 degrees C. This inhibition did not occur at the viral binding stage, as no difference was observed in MNV-1 genomic copies collected from washed RAW 264.7 cells without and with B. adolescentis after co-incubation for 1 h at room temperature. Meanwhile, the presence of B. adolescentis decreased the binding of human NoV Gl.1 VLPs to both Caco-2 cells and HT-29 cells, while no reduction was induced for the binding of human NoV Gll.4 VLPs to Caco-2 cells.
Keywords
Bifidobacterium adolescentis, Norovirus, probiotics, mechanisms, LACTIC-ACID BACTERIA, FELINE CALICIVIRUS, MURINE NOROVIRUS, VIRUS-INFECTION, CELL-LINES, PROBIOTICS, FERMENTATION, INACTIVATION, INHIBITION, PREVENTION

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Citation

Please use this url to cite or link to this publication:

Chicago
Li, Dan, Adrien Breiman, Jacques le Pendu, and Mieke Uyttendaele. 2016. “Anti-viral Effect of Bifidobacterium Adolescentis Against Noroviruses.” Frontiers in Microbiology 7.
APA
Li, Dan, Breiman, A., le Pendu, J., & Uyttendaele, M. (2016). Anti-viral effect of Bifidobacterium adolescentis against noroviruses. FRONTIERS IN MICROBIOLOGY, 7.
Vancouver
1.
Li D, Breiman A, le Pendu J, Uyttendaele M. Anti-viral effect of Bifidobacterium adolescentis against noroviruses. FRONTIERS IN MICROBIOLOGY. 2016;7.
MLA
Li, Dan, Adrien Breiman, Jacques le Pendu, et al. “Anti-viral Effect of Bifidobacterium Adolescentis Against Noroviruses.” FRONTIERS IN MICROBIOLOGY 7 (2016): n. pag. Print.
@article{8165145,
  abstract     = {This study aims to investigate the effect of Bifidobacterium adolescentis against noroviruses (NoVs). Murine norovirus-1 (MNV-1) used as a surrogate was detected by plaque assay and RT-qPCR. Human NoV virus like particles (VLPs) were detected by cell-binding assay. It was shown that the presence of B. adolescentis could inhibit the multiplication of MNV-1 on RAW 264.7 cells within 48 h of co-incubation period at 37 degrees C. This inhibition did not occur at the viral binding stage, as no difference was observed in MNV-1 genomic copies collected from washed RAW 264.7 cells without and with B. adolescentis after co-incubation for 1 h at room temperature. Meanwhile, the presence of B. adolescentis decreased the binding of human NoV Gl.1 VLPs to both Caco-2 cells and HT-29 cells, while no reduction was induced for the binding of human NoV Gll.4 VLPs to Caco-2 cells.},
  articleno    = {864},
  author       = {Li, Dan and Breiman, Adrien and le Pendu, Jacques and Uyttendaele, Mieke},
  issn         = {1664-302X},
  journal      = {FRONTIERS IN MICROBIOLOGY},
  keyword      = {Bifidobacterium adolescentis,Norovirus,probiotics,mechanisms,LACTIC-ACID BACTERIA,FELINE CALICIVIRUS,MURINE NOROVIRUS,VIRUS-INFECTION,CELL-LINES,PROBIOTICS,FERMENTATION,INACTIVATION,INHIBITION,PREVENTION},
  language     = {eng},
  pages        = {7},
  title        = {Anti-viral effect of Bifidobacterium adolescentis against noroviruses},
  url          = {http://dx.doi.org/10.3389/fmicb.2016.00864},
  volume       = {7},
  year         = {2016},
}

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