Advanced search
1 file | 2.02 MB Add to list

Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization

Author
Organization
Project
Abstract
Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol (R), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol (R) enhanced mineralization with CaP and also increased compressive modulus. Seanol (R) and ALP interacted in a non-covalent manner. Release of Seanol (R) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.
Keywords
polyphenol, mineralization, hydrogel, antibacterial, composite, BROWN-ALGAE, IN-VITRO, LIGAND-BINDING, BONE, PHOSPHATASE, BIOMATERIAL, POLYPHENOLS, BEHAVIOR, CELLS, RAT

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.02 MB

Citation

Please use this url to cite or link to this publication:

MLA
Douglas, Timothy et al. “Enrichment of Enzymatically Mineralized Gellan Gum Hydrogels with Phlorotannin-rich Ecklonia Cava Extract Seanol® to Endow Antibacterial Properties and Promote Mineralization.” BIOMEDICAL MATERIALS 11.4 (2016): n. pag. Print.
APA
Douglas, T., Dokupil, A., Reczyńska, K., Brackman, G., Krok-Borkowicz, M., Keppler, J. K., Božič, M., et al. (2016). Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization. BIOMEDICAL MATERIALS, 11(4).
Chicago author-date
Douglas, Timothy, Agnieszka Dokupil, Katarzyna Reczyńska, Gilles Brackman, Malgorzata Krok-Borkowicz, Julia K Keppler, Mojca Božič, et al. 2016. “Enrichment of Enzymatically Mineralized Gellan Gum Hydrogels with Phlorotannin-rich Ecklonia Cava Extract Seanol® to Endow Antibacterial Properties and Promote Mineralization.” Biomedical Materials 11 (4).
Chicago author-date (all authors)
Douglas, Timothy, Agnieszka Dokupil, Katarzyna Reczyńska, Gilles Brackman, Malgorzata Krok-Borkowicz, Julia K Keppler, Mojca Božič, Pascal Van Der Voort, Krzysztof Pietryga, Sangram Keshari Samal, Lieve Balcaen, Jan Van den Bulcke, Joris Van Acker, Frank Vanhaecke, Karin Schwarz, Tom Coenye, and Elżbieta Pamuła. 2016. “Enrichment of Enzymatically Mineralized Gellan Gum Hydrogels with Phlorotannin-rich Ecklonia Cava Extract Seanol® to Endow Antibacterial Properties and Promote Mineralization.” Biomedical Materials 11 (4).
Vancouver
1.
Douglas T, Dokupil A, Reczyńska K, Brackman G, Krok-Borkowicz M, Keppler JK, et al. Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization. BIOMEDICAL MATERIALS. 2016;11(4).
IEEE
[1]
T. Douglas et al., “Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization,” BIOMEDICAL MATERIALS, vol. 11, no. 4, 2016.
@article{8163840,
  abstract     = {Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol (R), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol (R) enhanced mineralization with CaP and also increased compressive modulus. Seanol (R) and ALP interacted in a non-covalent manner. Release of Seanol (R) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.},
  articleno    = {045015},
  author       = {Douglas, Timothy and Dokupil, Agnieszka and Reczyńska, Katarzyna and Brackman, Gilles and Krok-Borkowicz, Malgorzata and Keppler, Julia K and Božič, Mojca and Van Der Voort, Pascal and Pietryga, Krzysztof and Samal, Sangram Keshari and Balcaen, Lieve and Van den Bulcke, Jan and Van Acker, Joris and Vanhaecke, Frank and Schwarz, Karin and Coenye, Tom and Pamuła, Elżbieta},
  issn         = {1748-6041},
  journal      = {BIOMEDICAL MATERIALS},
  keywords     = {polyphenol,mineralization,hydrogel,antibacterial,composite,BROWN-ALGAE,IN-VITRO,LIGAND-BINDING,BONE,PHOSPHATASE,BIOMATERIAL,POLYPHENOLS,BEHAVIOR,CELLS,RAT},
  language     = {eng},
  number       = {4},
  pages        = {13},
  title        = {Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization},
  url          = {http://dx.doi.org/10.1088/1748-6041/11/4/045015},
  volume       = {11},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: