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Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization

Timothy Douglas, Agnieszka Dokupil, Katarzyna Reczyńska, Gilles Brackman, Malgorzata Krok-Borkowicz, Julia K Keppler, Mojca Božič, Pascal Van Der Voort UGent, Krzysztof Pietryga, Sangram Keshari Samal, et al. (2016) BIOMEDICAL MATERIALS. 11(4).
abstract
Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol (R), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP_min, GG/Seanol_min and GG/Seanol/ALP_min. Seanol (R) enhanced mineralization with CaP and also increased compressive modulus. Seanol (R) and ALP interacted in a non-covalent manner. Release of Seanol (R) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP_min, but not GG/Seanol_min, retained some antibacterial activity. Eluates taken from groups GG/ALP_min, GG/Seanol_min and GG/ALP/Seanol_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.
Please use this url to cite or link to this publication:
author
organization
alternative title
Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol (R) to endow antibacterial properties and promote mineralization
year
type
journalArticle (original)
publication status
published
subject
keyword
polyphenol, mineralization, hydrogel, antibacterial, composite, BROWN-ALGAE, IN-VITRO, LIGAND-BINDING, BONE, PHOSPHATASE, BIOMATERIAL, POLYPHENOLS, BEHAVIOR, CELLS, RAT
journal title
BIOMEDICAL MATERIALS
Biomed. Mater.
volume
11
issue
4
article number
045015
pages
13 pages
Web of Science type
Article
Web of Science id
000383288600016
JCR category
ENGINEERING, BIOMEDICAL
JCR impact factor
2.469 (2016)
JCR rank
27/77 (2016)
JCR quartile
2 (2016)
ISSN
1748-6041
DOI
10.1088/1748-6041/11/4/045015
project
Center for nano- and biophotonics (NB-Photonics)
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8163840
handle
http://hdl.handle.net/1854/LU-8163840
date created
2016-11-22 17:06:21
date last changed
2018-01-29 12:12:54
@article{8163840,
  abstract     = {Hydrogels offer several advantages as biomaterials for bone regeneration, including ease of incorporation of soluble substances such as mineralization-promoting enzymes and antibacterial agents. Mineralization with calcium phosphate (CaP) increases bioactivity, while antibacterial activity reduces the risk of infection. Here, gellan gum (GG) hydrogels were enriched with alkaline phosphatase (ALP) and/or Seanol (R), a seaweed extract rich in phlorotannins (brown algae-derived polyphenols), to induce mineralization with CaP and increase antibacterial activity, respectively. The sample groups were unmineralized hydrogels, denoted as GG, GG/ALP, GG/Seanol and GG/Seanol/ALP, and hydrogels incubated in mineralization medium (0.1 M calcium glycerophosphate), denoted as GG/ALP\_min, GG/Seanol\_min and GG/Seanol/ALP\_min. Seanol (R) enhanced mineralization with CaP and also increased compressive modulus. Seanol (R) and ALP interacted in a non-covalent manner. Release of Seanol (R) occurred in a burst phase and was impeded by ALP-mediated mineralization. Groups GG/Seanol and GG/ALP/Seanol exhibited antibacterial activity against methicillin-resistant Staphylococcus aureus. GG/Seanol/ALP\_min, but not GG/Seanol\_min, retained some antibacterial activity. Eluates taken from groups GG/ALP\_min, GG/Seanol\_min and GG/ALP/Seanol\_min displayed comparable cytotoxicity towards MG-63 osteoblast-like cells. These results suggest that enrichment of hydrogel biomaterials with phlorotannin-rich extracts is a promising strategy to increase mineralizability and antibacterial activity.},
  articleno    = {045015},
  author       = {Douglas, Timothy and Dokupil, Agnieszka and Reczy\'{n}ska, Katarzyna and Brackman, Gilles and Krok-Borkowicz, Malgorzata and Keppler, Julia K and Bo\v{z}i\v{c}, Mojca and Van Der Voort, Pascal and Pietryga, Krzysztof and Samal, Sangram Keshari and Balcaen, Lieve and Van den Bulcke, Jan and Van Acker, Joris and Vanhaecke, Frank and Schwarz, Karin and Coenye, Tom and Pamu\unmatched{0142}a, El\.{z}bieta},
  issn         = {1748-6041},
  journal      = {BIOMEDICAL MATERIALS},
  keyword      = {polyphenol,mineralization,hydrogel,antibacterial,composite,BROWN-ALGAE,IN-VITRO,LIGAND-BINDING,BONE,PHOSPHATASE,BIOMATERIAL,POLYPHENOLS,BEHAVIOR,CELLS,RAT},
  language     = {eng},
  number       = {4},
  pages        = {13},
  title        = {Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol{\textregistered} to endow antibacterial properties and promote mineralization},
  url          = {http://dx.doi.org/10.1088/1748-6041/11/4/045015},
  volume       = {11},
  year         = {2016},
}

Chicago
Douglas, Timothy, Agnieszka Dokupil, Katarzyna Reczyńska, Gilles Brackman, Malgorzata Krok-Borkowicz, Julia K Keppler, Mojca Božič, et al. 2016. “Enrichment of Enzymatically Mineralized Gellan Gum Hydrogels with Phlorotannin-rich Ecklonia Cava Extract Seanol® to Endow Antibacterial Properties and Promote Mineralization.” Biomedical Materials 11 (4).
APA
Douglas, Timothy, Dokupil, A., Reczyńska, K., Brackman, G., Krok-Borkowicz, M., Keppler, J. K., Božič, M., et al. (2016). Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization. BIOMEDICAL MATERIALS, 11(4).
Vancouver
1.
Douglas T, Dokupil A, Reczyńska K, Brackman G, Krok-Borkowicz M, Keppler JK, et al. Enrichment of enzymatically mineralized gellan gum hydrogels with phlorotannin-rich Ecklonia cava extract Seanol® to endow antibacterial properties and promote mineralization. BIOMEDICAL MATERIALS. 2016;11(4).
MLA
Douglas, Timothy, Agnieszka Dokupil, Katarzyna Reczyńska, et al. “Enrichment of Enzymatically Mineralized Gellan Gum Hydrogels with Phlorotannin-rich Ecklonia Cava Extract Seanol® to Endow Antibacterial Properties and Promote Mineralization.” BIOMEDICAL MATERIALS 11.4 (2016): n. pag. Print.