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Characterization of a new pink-fruited tomato mutant results in the identification of a null allele of the SlMYB12 transcription factor

(2016) PLANT PHYSIOLOGY. 171(3). p.1821-1836
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Abstract
The identification and characterization of new tomato (Solanum lycopersicum) mutants affected in fruit pigmentation and nutritional content can provide valuable insights into the underlying biology, as well as a source of new alleles for breeding programs. To date, all characterized pink-pigmented tomato fruit mutants appear to result from low SlMYB12 transcript levels in the fruit skin. Two new mutant lines displaying a pink fruit phenotype (pf1 and pf2) were characterized in this study. In the pf mutants, SlMYB12 transcripts accumulated to wild-type levels but exhibited the same truncation, which resulted in the absence of the essential MYB activation domain coding region. Allelism and complementation tests revealed that both pf mutants were allelic to the y locus and showed the same recessive null allele in homozygosis: Delta y. A set of molecular and metabolic effects, reminiscent of those observed in the Arabidopsis (Arabidopsis thaliana) myb11 myb12 myb111 triple mutant, were found in the tomato Delta y mutants. To our knowledge, these have not been described previously, and our data support the idea of their being null mutants, in contrast to previously described transcriptional hypomorphic pink fruit lines. We detected a reduction in the expression of several flavonol glycosides and some associated glycosyl transferases. Transcriptome analysis further revealed that the effects of the pf mutations extended beyond the flavonoid pathway into the interface between primary and secondary metabolism. Finally, screening for Myb-binding sites in the candidate gene promoter sequences revealed that 141 of the 152 co-down-regulated genes may be direct targets of SlMYB12 regulation.
Keywords
ARABIDOPSIS-THALIANA, FLAVONOID BIOSYNTHESIS, PHENYLPROPANOID BIOSYNTHESIS, POLYKETIDE SYNTHASES, SOLANUM-LYCOPERSICON, CHALCONE SYNTHASE, MOTIF DISCOVERY, P-GENE, EXPRESSION, R2R3-MYB

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Citation

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Chicago
Fernandez-Moreno, Josefina-Patricia, Oren Tzfadia, Javier Forment, Silvia Presa, Ilana Rogachev, Sagit Meir, Diego Orzaez, Aspah Aharoni, and Antonio Granell. 2016. “Characterization of a New Pink-fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.” Plant Physiology 171 (3): 1821–1836.
APA
Fernandez-Moreno, J.-P., Tzfadia, O., Forment, J., Presa, S., Rogachev, I., Meir, S., Orzaez, D., et al. (2016). Characterization of a new pink-fruited tomato mutant results in the identification of a null allele of the SlMYB12 transcription factor. PLANT PHYSIOLOGY, 171(3), 1821–1836.
Vancouver
1.
Fernandez-Moreno J-P, Tzfadia O, Forment J, Presa S, Rogachev I, Meir S, et al. Characterization of a new pink-fruited tomato mutant results in the identification of a null allele of the SlMYB12 transcription factor. PLANT PHYSIOLOGY. 2016;171(3):1821–36.
MLA
Fernandez-Moreno, Josefina-Patricia, Oren Tzfadia, Javier Forment, et al. “Characterization of a New Pink-fruited Tomato Mutant Results in the Identification of a Null Allele of the SlMYB12 Transcription Factor.” PLANT PHYSIOLOGY 171.3 (2016): 1821–1836. Print.
@article{8157494,
  abstract     = {The identification and characterization of new tomato (Solanum lycopersicum) mutants affected in fruit pigmentation and nutritional content can provide valuable insights into the underlying biology, as well as a source of new alleles for breeding programs. To date, all characterized pink-pigmented tomato fruit mutants appear to result from low SlMYB12 transcript levels in the fruit skin. Two new mutant lines displaying a pink fruit phenotype (pf1 and pf2) were characterized in this study. In the pf mutants, SlMYB12 transcripts accumulated to wild-type levels but exhibited the same truncation, which resulted in the absence of the essential MYB activation domain coding region. Allelism and complementation tests revealed that both pf mutants were allelic to the y locus and showed the same recessive null allele in homozygosis: Delta y. A set of molecular and metabolic effects, reminiscent of those observed in the Arabidopsis (Arabidopsis thaliana) myb11 myb12 myb111 triple mutant, were found in the tomato Delta y mutants. To our knowledge, these have not been described previously, and our data support the idea of their being null mutants, in contrast to previously described transcriptional hypomorphic pink fruit lines. We detected a reduction in the expression of several flavonol glycosides and some associated glycosyl transferases. Transcriptome analysis further revealed that the effects of the pf mutations extended beyond the flavonoid pathway into the interface between primary and secondary metabolism. Finally, screening for Myb-binding sites in the candidate gene promoter sequences revealed that 141 of the 152 co-down-regulated genes may be direct targets of SlMYB12 regulation.},
  author       = {Fernandez-Moreno, Josefina-Patricia and Tzfadia, Oren and Forment, Javier and Presa, Silvia and Rogachev, Ilana and Meir, Sagit and Orzaez, Diego and Aharoni, Aspah and Granell, Antonio},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  keyword      = {ARABIDOPSIS-THALIANA,FLAVONOID BIOSYNTHESIS,PHENYLPROPANOID BIOSYNTHESIS,POLYKETIDE SYNTHASES,SOLANUM-LYCOPERSICON,CHALCONE SYNTHASE,MOTIF DISCOVERY,P-GENE,EXPRESSION,R2R3-MYB},
  language     = {eng},
  number       = {3},
  pages        = {1821--1836},
  title        = {Characterization of a new pink-fruited tomato mutant results in the identification of a null allele of the SlMYB12 transcription factor},
  url          = {http://dx.doi.org/10.1104/pp.16.00282},
  volume       = {171},
  year         = {2016},
}

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