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Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty acid composition of delta5 and delta6 desaturation in tissues of female piglets

Joris Missotten UGent, Stefaan De Smet UGent, Katleen Raes UGent and Olena Doran (2009) ANIMAL. 3(8). p.1196-1204
abstract
The present study investigated whether enrichment of the pig maternal diet with n-3 polyunsaturated fatty acids (PUFA) affects the fatty-acid composition of female piglets via enhancing of expression of the lipogenic enzymes Delta 5-desaturase (Delta 5d) and Delta 6-desaturase (Delta 6d). The sows (50% Landrace X 50% Large White) were fed a control diet or one of the experimental diets starting at day 45 in gestation. The experimental diets were supplemented either with linseed oil or fish oil, whereas the control diet contained palm oil. Expression of Delta 5d and Delta 6d, and fatty-acid composition was determined by Western blotting and gas-liquid chromatography, respectively, in muscle, subcutaneous adipose tissue and liver. The highest Delta 5d protein expression was observed in the piglets' muscle, followed by subcutaneous adipose tissue, with the lowest level in the liver Expression of Delta 6d in the piglets' tissues followed an opposite pattern, and was highest in the liver followed by subcutaneous adipose tissue, with the lowest level in muscle. Supplementation of the maternal diet with fish oil or linseed oil increased the level of n-3 PUFA of the piglets in a tissue-specific manner The response of Delta 6d and Delta 5d protein expression in female piglets, with average birth weight 2.4 kg, to the dietary manipulation was also tissue-specific. It is suggested that the increase in n-3 PUFA content in the progeny was related, at least partially, to the activation of Delta 6d and Delta 5d expression.
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author
organization
alternative title
Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty-acid composition and expression of Delta 5-and Delta 6-desaturase in tissues of female piglets
year
type
journalArticle (original)
publication status
published
subject
keyword
ADIPOSE-TISSUE, LIPID-COMPOSITION, MEAT QUALITY, N-3, DESATURASE, DOCOSAHEXAENOIC ACID, CONJUGATED LINOLEIC-ACID, ENZYME GENE-EXPRESSION, lipogenic enzyme, protein expression, fatty-acid composition, diet, pig, NUTRITIONAL REGULATION, SUDDEN CARDIAC DEATH
journal title
ANIMAL
Animal
volume
3
issue
8
pages
1196 - 1204
Web of Science type
Article
Web of Science id
000268079600015
JCR category
VETERINARY SCIENCES
JCR impact factor
1.461 (2009)
JCR rank
34/141 (2009)
JCR quartile
1 (2009)
ISSN
1751-7311
DOI
10.1017/S1751731109004455
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
814823
handle
http://hdl.handle.net/1854/LU-814823
date created
2009-12-21 14:26:40
date last changed
2010-09-01 00:30:18
@article{814823,
  abstract     = {The present study investigated whether enrichment of the pig maternal diet with n-3 polyunsaturated fatty acids (PUFA) affects the fatty-acid composition of female piglets via enhancing of expression of the lipogenic enzymes Delta 5-desaturase (Delta 5d) and Delta 6-desaturase (Delta 6d). The sows (50\% Landrace X 50\% Large White) were fed a control diet or one of the experimental diets starting at day 45 in gestation. The experimental diets were supplemented either with linseed oil or fish oil, whereas the control diet contained palm oil. Expression of Delta 5d and Delta 6d, and fatty-acid composition was determined by Western blotting and gas-liquid chromatography, respectively, in muscle, subcutaneous adipose tissue and liver. The highest Delta 5d protein expression was observed in the piglets' muscle, followed by subcutaneous adipose tissue, with the lowest level in the liver Expression of Delta 6d in the piglets' tissues followed an opposite pattern, and was highest in the liver followed by subcutaneous adipose tissue, with the lowest level in muscle. Supplementation of the maternal diet with fish oil or linseed oil increased the level of n-3 PUFA of the piglets in a tissue-specific manner The response of Delta 6d and Delta 5d protein expression in female piglets, with average birth weight 2.4 kg, to the dietary manipulation was also tissue-specific. It is suggested that the increase in n-3 PUFA content in the progeny was related, at least partially, to the activation of Delta 6d and Delta 5d expression.},
  author       = {Missotten, Joris and De Smet, Stefaan and Raes, Katleen and Doran, Olena},
  issn         = {1751-7311},
  journal      = {ANIMAL},
  keyword      = {ADIPOSE-TISSUE,LIPID-COMPOSITION,MEAT QUALITY,N-3,DESATURASE,DOCOSAHEXAENOIC ACID,CONJUGATED LINOLEIC-ACID,ENZYME GENE-EXPRESSION,lipogenic enzyme,protein expression,fatty-acid composition,diet,pig,NUTRITIONAL REGULATION,SUDDEN CARDIAC DEATH},
  language     = {eng},
  number       = {8},
  pages        = {1196--1204},
  title        = {Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty acid composition of delta5 and delta6 desaturation in tissues of female piglets},
  url          = {http://dx.doi.org/10.1017/S1751731109004455},
  volume       = {3},
  year         = {2009},
}

Chicago
Missotten, Joris, Stefaan De Smet, Katleen Raes, and Olena Doran. 2009. “Effect of Supplementation of the Maternal Diet with Fish Oil or Linseed Oil on Fatty Acid Composition of Delta5 and Delta6 Desaturation in Tissues of Female Piglets.” Animal 3 (8): 1196–1204.
APA
Missotten, J., De Smet, S., Raes, K., & Doran, O. (2009). Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty acid composition of delta5 and delta6 desaturation in tissues of female piglets. ANIMAL, 3(8), 1196–1204.
Vancouver
1.
Missotten J, De Smet S, Raes K, Doran O. Effect of supplementation of the maternal diet with fish oil or linseed oil on fatty acid composition of delta5 and delta6 desaturation in tissues of female piglets. ANIMAL. 2009;3(8):1196–204.
MLA
Missotten, Joris, Stefaan De Smet, Katleen Raes, et al. “Effect of Supplementation of the Maternal Diet with Fish Oil or Linseed Oil on Fatty Acid Composition of Delta5 and Delta6 Desaturation in Tissues of Female Piglets.” ANIMAL 3.8 (2009): 1196–1204. Print.