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Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay)

Author
Organization
Project
  • RENEB (Realizing the European network in Biodosimetry (RENEB))
Abstract
Purpose: Dose assessment intercomparisons within the RENEB network were performed for triage biodosimetry analyzing G0-lymphocyte PCC for harmonization, standardization and optimization of the PCC assay. Materials and methods: Comparative analysis among different partners for dose assessment included shipment of PCC-slides and captured images to construct dose-response curves for up to 6 Gy c-rays. Accident simulation exercises were performed to assess the suitability of the PCC assay by detecting speed of analysis and minimum number of cells required for categorization of potentially exposed individuals. Results: Calibration data based on Giemsa-stained fragments in excess of 46 PCC were obtained by different partners using galleries of PCC images for each dose-point. Mean values derived from all scores yielded a linear dose-response with approximately 4 excess-fragments/cell/Gy. To unify scoring criteria, exercises were carried out using coded PCC-slides and/or coded irradiated blood samples. Analysis of samples received 24 h post-exposure was successfully performed using Giemsa staining (1 excess-fragment/cell/Gy) or centromere/telomere FISH-staining for dicentrics. Conclusions: Dose assessments by RENEB partners using appropriate calibration curves were mostly in good agreement. The PCC assay is quick and reliable for whole- or partial-body triage biodosimetry by scoring excess-fragments or dicentrics in G0-lymphocytes. Particularly, analysis of Giemsa-stained excess PCC-fragments is simple, inexpensive and its automation could increase throughput and scoring objectivity of the PCC assay.
Keywords
Biodosimetry, centromere/telomere PNA FISH, cell fusion, premature chromosome condensation, PCC assay, PERIPHERAL-BLOOD LYMPHOCYTES, IRRADIATED HUMAN-LYMPHOCYTES, BIOLOGICAL DOSIMETRY, TRIAGE BIODOSIMETRY, RADIATION, FISH, COMBINATION, EXCHANGES, FRAGMENTS, PROBES

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Citation

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MLA
Terzoudi, Georgia I., et al. “Dose Assessment Intercomparisons within the RENEB Network Using G0-Lymphocyte Prematurely Condensed Chromosomes (PCC Assay).” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, vol. 93, no. 1, 2017, pp. 48–57, doi:10.1080/09553002.2016.1234725.
APA
Terzoudi, G. I., Pantelias, G., Darroudi, F., Barszczewska, K., Buraczewska, I., Depuydt, J., … Wojcik, A. (2017). Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 93(1), 48–57. https://doi.org/10.1080/09553002.2016.1234725
Chicago author-date
Terzoudi, Georgia I, Gabriel Pantelias, Firouz Darroudi, Katarzyna Barszczewska, Iwona Buraczewska, Julie Depuydt, Dimka Georgieva, et al. 2017. “Dose Assessment Intercomparisons within the RENEB Network Using G0-Lymphocyte Prematurely Condensed Chromosomes (PCC Assay).” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 93 (1): 48–57. https://doi.org/10.1080/09553002.2016.1234725.
Chicago author-date (all authors)
Terzoudi, Georgia I, Gabriel Pantelias, Firouz Darroudi, Katarzyna Barszczewska, Iwona Buraczewska, Julie Depuydt, Dimka Georgieva, Valeria Hadjidekova, Vasiliki I Hatzi, Ioanna Karachristou, Maria Karakosta, Roberta Meschini, Radhia M’Kacher, Alegria Montoro, Fabrizio Palitti, Antonio Pantelias, Gaetano Pepe, Michelle Ricoul, Laure Sabatier, Natividad Sebastià, Sylwester Sommer, Anne Vral, Demetre Zafiropoulos, and Andrzej Wojcik. 2017. “Dose Assessment Intercomparisons within the RENEB Network Using G0-Lymphocyte Prematurely Condensed Chromosomes (PCC Assay).” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 93 (1): 48–57. doi:10.1080/09553002.2016.1234725.
Vancouver
1.
Terzoudi GI, Pantelias G, Darroudi F, Barszczewska K, Buraczewska I, Depuydt J, et al. Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2017;93(1):48–57.
IEEE
[1]
G. I. Terzoudi et al., “Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay),” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, vol. 93, no. 1, pp. 48–57, 2017.
@article{8136990,
  abstract     = {{Purpose: Dose assessment intercomparisons within the RENEB network were performed for triage biodosimetry analyzing G0-lymphocyte PCC for harmonization, standardization and optimization of the PCC assay.
Materials and methods: Comparative analysis among different partners for dose assessment included shipment of PCC-slides and captured images to construct dose-response curves for up to 6 Gy c-rays. Accident simulation exercises were performed to assess the suitability of the PCC assay by detecting speed of analysis and minimum number of cells required for categorization of potentially exposed individuals.
Results: Calibration data based on Giemsa-stained fragments in excess of 46 PCC were obtained by different partners using galleries of PCC images for each dose-point. Mean values derived from all scores yielded a linear dose-response with approximately 4 excess-fragments/cell/Gy. To unify scoring criteria, exercises were carried out using coded PCC-slides and/or coded irradiated blood samples. Analysis of samples received 24 h post-exposure was successfully performed using Giemsa staining (1 excess-fragment/cell/Gy) or centromere/telomere FISH-staining for dicentrics.
Conclusions: Dose assessments by RENEB partners using appropriate calibration curves were mostly in good agreement. The PCC assay is quick and reliable for whole- or partial-body triage biodosimetry by scoring excess-fragments or dicentrics in G0-lymphocytes. Particularly, analysis of Giemsa-stained excess PCC-fragments is simple, inexpensive and its automation could increase throughput and scoring objectivity of the PCC assay.}},
  author       = {{Terzoudi, Georgia I and Pantelias, Gabriel and Darroudi, Firouz and Barszczewska, Katarzyna and Buraczewska, Iwona and Depuydt, Julie and Georgieva, Dimka and Hadjidekova, Valeria and Hatzi, Vasiliki I and Karachristou, Ioanna and Karakosta, Maria and Meschini, Roberta and M’Kacher, Radhia and Montoro, Alegria and Palitti, Fabrizio and Pantelias, Antonio and Pepe, Gaetano and Ricoul, Michelle and Sabatier, Laure and Sebastià, Natividad and Sommer, Sylwester and Vral, Anne and Zafiropoulos, Demetre and Wojcik, Andrzej}},
  issn         = {{0955-3002}},
  journal      = {{INTERNATIONAL JOURNAL OF RADIATION BIOLOGY}},
  keywords     = {{Biodosimetry,centromere/telomere PNA FISH,cell fusion,premature chromosome condensation,PCC assay,PERIPHERAL-BLOOD LYMPHOCYTES,IRRADIATED HUMAN-LYMPHOCYTES,BIOLOGICAL DOSIMETRY,TRIAGE BIODOSIMETRY,RADIATION,FISH,COMBINATION,EXCHANGES,FRAGMENTS,PROBES}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{48--57}},
  title        = {{Dose assessment intercomparisons within the RENEB network using G0-lymphocyte prematurely condensed chromosomes (PCC assay)}},
  url          = {{http://doi.org/10.1080/09553002.2016.1234725}},
  volume       = {{93}},
  year         = {{2017}},
}

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