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The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)

Jayne Moquet, Stephen Barnard, Albena Staynova, Carita Lindholm, Octávia Monteiro Gil, Vanda Martins, Ute Rößler, Anne Vral UGent, Charlot Vandevoorde, Maria Wojewódzka, et al. (2017) INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 93(1). p.58-64
abstract
Purpose: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. Materials and methods: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples. Results: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty. Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
gamma-H2AX assay, radiation-induced foci, biological dosimetry, RENEB, triage, RADIATION EMERGENCY, HUMAN-LYMPHOCYTES, DNA-DAMAGE, FOCI, EXPOSURE, LABORATORIES, IRRADIATION, DOSIMETRY, TRIAGE
journal title
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
Int. J. Radiat. Biol.
volume
93
issue
1
issue title
Networking in biological and EPR/OSL dosimetry : the European RENEB platform for emergency preparedness and research
pages
58 - 64
Web of Science type
Article
Web of Science id
000392891400008
ISSN
0955-3002
DOI
10.1080/09553002.2016.1207822
project
RENEB (Realizing the European Network in Biodosimetry)
language
English
UGent publication?
yes
classification
A1
copyright statement
Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
id
8136614
handle
http://hdl.handle.net/1854/LU-8136614
date created
2016-11-09 11:40:15
date last changed
2017-04-25 07:01:06
@article{8136614,
  abstract     = {Purpose: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise.
Materials and methods: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples.
Results: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty.
Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.},
  author       = {Moquet, Jayne and Barnard, Stephen and Staynova, Albena and Lindholm, Carita and Monteiro Gil, Oct{\'a}via and Martins, Vanda and R{\"o}{\ss}ler, Ute and Vral, Anne and Vandevoorde, Charlot and Wojew{\'o}dzka, Maria and Rothkamm, Kai},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {gamma-H2AX assay,radiation-induced foci,biological dosimetry,RENEB,triage,RADIATION EMERGENCY,HUMAN-LYMPHOCYTES,DNA-DAMAGE,FOCI,EXPOSURE,LABORATORIES,IRRADIATION,DOSIMETRY,TRIAGE},
  language     = {eng},
  number       = {1},
  pages        = {58--64},
  title        = {The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)},
  url          = {http://dx.doi.org/10.1080/09553002.2016.1207822},
  volume       = {93},
  year         = {2017},
}

Chicago
Moquet, Jayne, Stephen Barnard, Albena Staynova, Carita Lindholm, Octávia Monteiro Gil, Vanda Martins, Ute Rößler, et al. 2017. “The Second gamma-H2AX Assay Inter-comparison Exercise Carried Out in the Framework of the European Biodosimetry Network (RENEB).” International Journal of Radiation Biology 93 (1): 58–64.
APA
Moquet, J., Barnard, S., Staynova, A., Lindholm, C., Monteiro Gil, O., Martins, V., Rößler, U., et al. (2017). The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 93(1), 58–64.
Vancouver
1.
Moquet J, Barnard S, Staynova A, Lindholm C, Monteiro Gil O, Martins V, et al. The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2017;93(1):58–64.
MLA
Moquet, Jayne, Stephen Barnard, Albena Staynova, et al. “The Second gamma-H2AX Assay Inter-comparison Exercise Carried Out in the Framework of the European Biodosimetry Network (RENEB).” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 93.1 (2017): 58–64. Print.