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The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)

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Project
RENEB (Realizing the European Network in Biodosimetry)
Abstract
Purpose: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise. Materials and methods: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples. Results: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty. Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.
Keywords
gamma-H2AX assay, radiation-induced foci, biological dosimetry, RENEB, triage, RADIATION EMERGENCY, HUMAN-LYMPHOCYTES, DNA-DAMAGE, FOCI, EXPOSURE, LABORATORIES, IRRADIATION, DOSIMETRY, TRIAGE

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Citation

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Chicago
Moquet, Jayne, Stephen Barnard, Albena Staynova, Carita Lindholm, Octávia Monteiro Gil, Vanda Martins, Ute Rößler, et al. 2017. “The Second gamma-H2AX Assay Inter-comparison Exercise Carried Out in the Framework of the European Biodosimetry Network (RENEB).” International Journal of Radiation Biology 93 (1): 58–64.
APA
Moquet, J., Barnard, S., Staynova, A., Lindholm, C., Monteiro Gil, O., Martins, V., Rößler, U., et al. (2017). The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 93(1), 58–64.
Vancouver
1.
Moquet J, Barnard S, Staynova A, Lindholm C, Monteiro Gil O, Martins V, et al. The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB). INTERNATIONAL JOURNAL OF RADIATION BIOLOGY. 2017;93(1):58–64.
MLA
Moquet, Jayne, Stephen Barnard, Albena Staynova, et al. “The Second gamma-H2AX Assay Inter-comparison Exercise Carried Out in the Framework of the European Biodosimetry Network (RENEB).” INTERNATIONAL JOURNAL OF RADIATION BIOLOGY 93.1 (2017): 58–64. Print.
@article{8136614,
  abstract     = {Purpose: Within the EU RENEB project, seven laboratories have taken part in training and harmonisation activities to strengthen triage gamma H2AX-based radiation exposure assessment. This has culminated in a second triage biodosimetry exercise.
Materials and methods: Whole blood and separated lymphocyte samples were homogenously irradiated with 60Co gamma rays at 0.5, 2.5 (blind samples), 0 and 2 Gy (reference samples). Following post-exposure incubations of 4 and 24 h, 16 samples were shipped on ice packs to each partner. The samples were stained and scored for gamma-H2AX foci, using manual and/or automated fluorescence microscope scoring strategies. Dose estimates were obtained and used to assign triage categories to the samples.
Results: Average dose estimates across all the laboratories correlated well with true doses. The most accurate assignment of triage category was achieved by manual scoring of the 4-h blood and lymphocyte samples. Only three samples out of a total of 46 were miscategorized in a way that could have adversely effected the clinical management of a radiation casualty.
Conclusions: This inter-comparison exercise has demonstrated that following a recent acute radiation exposure, the gamma-H2AX assay could be a useful triage tool that can be successfully applied across a network of laboratories.},
  author       = {Moquet, Jayne and Barnard, Stephen and Staynova, Albena and Lindholm, Carita and Monteiro Gil, Oct{\'a}via and Martins, Vanda and R{\"o}{\ss}ler, Ute and Vral, Anne and Vandevoorde, Charlot and Wojew{\'o}dzka, Maria and Rothkamm, Kai},
  issn         = {0955-3002},
  journal      = {INTERNATIONAL JOURNAL OF RADIATION BIOLOGY},
  keyword      = {gamma-H2AX assay,radiation-induced foci,biological dosimetry,RENEB,triage,RADIATION EMERGENCY,HUMAN-LYMPHOCYTES,DNA-DAMAGE,FOCI,EXPOSURE,LABORATORIES,IRRADIATION,DOSIMETRY,TRIAGE},
  language     = {eng},
  number       = {1},
  pages        = {58--64},
  title        = {The second gamma-H2AX assay inter-comparison exercise carried out in the framework of the European biodosimetry network (RENEB)},
  url          = {http://dx.doi.org/10.1080/09553002.2016.1207822},
  volume       = {93},
  year         = {2017},
}

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