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Wheat bran components modulate intestinal bacteria and gene expression of barrier function relevant proteins in a piglet model

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Abstract
The objective of this study was to determine the impact of wheat bran and its main polysaccharides on intestinal bacteria and gene expression of intestinal barrier function relevant proteins. Thirty freshly weaned male piglets were assigned randomly to five dietary treatment groups with six piglets per group. Accordingly, five synthetic diets including a basal control diet without fiber components (CON), wheat bran diet (10% wheat bran, WB), arabinoxylan diet (AX), cellulose diet (CEL) and combined diet of arabinoxylan and cellulose (CB) were studied. The piglets were fed ad libitum for 30 d. Lower Escherichia coli (E. coli) populations in WB group and higher probiotic (Lactobacillus and Bifidobacterium) populations in groups fed diets containing arabinoxylan (WB, AX and CB) were observed and compared with CON group. Compared with CON group, the gene expressions of cystic fibrosis transmembrane conductance regulator (CFTR), calcium-activated chloride channel regulator 1 (CLCA1) and voltage-gated chloride channel 2 (CIC2) were suppressed in the WB group. And wheat bran down-regulated gene expression of pro-inflammation (TNF-alpha, IL-1 beta, IL-6) and TLRs/MyD88/NF-kappa B pathway compared with CON group. In conclusion, wheat bran and its main polysaccharides could change intestinal microflora and down-regulate the gene expression of intestinal barrier function relevant proteins in the distal small intestinal mucosa.
Keywords
Arabinoxylan, cellulose, chloride channel, gene expression, NF-KAPPA-B, GALANIN-1 RECEPTOR EXPRESSION, CHLORIDE SECRETION, DIETARY FIBER, ACTIVATION, COLITIS, ARABINOXYLAN, TUBERCULOSIS, PATHWAY, ALPHA

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Chicago
Chen, Hong, Daiwen Chen, Wen Qin, Yuntao Liu, Lianqiang Che, Zhiqing Huang, Yuheng Luo, et al. 2017. “Wheat Bran Components Modulate Intestinal Bacteria and Gene Expression of Barrier Function Relevant Proteins in a Piglet Model.” International Journal of Food Sciences and Nutrition 68 (1): 65–72.
APA
Chen, Hong, Chen, D., Qin, W., Liu, Y., Che, L., Huang, Z., Luo, Y., et al. (2017). Wheat bran components modulate intestinal bacteria and gene expression of barrier function relevant proteins in a piglet model. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 68(1), 65–72.
Vancouver
1.
Chen H, Chen D, Qin W, Liu Y, Che L, Huang Z, et al. Wheat bran components modulate intestinal bacteria and gene expression of barrier function relevant proteins in a piglet model. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION. 2017;68(1):65–72.
MLA
Chen, Hong, Daiwen Chen, Wen Qin, et al. “Wheat Bran Components Modulate Intestinal Bacteria and Gene Expression of Barrier Function Relevant Proteins in a Piglet Model.” INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION 68.1 (2017): 65–72. Print.
@article{8133532,
  abstract     = {The objective of this study was to determine the impact of wheat bran and its main polysaccharides on intestinal bacteria and gene expression of intestinal barrier function relevant proteins. Thirty freshly weaned male piglets were assigned randomly to five dietary treatment groups with six piglets per group. Accordingly, five synthetic diets including a basal control diet without fiber components (CON), wheat bran diet (10\% wheat bran, WB), arabinoxylan diet (AX), cellulose diet (CEL) and combined diet of arabinoxylan and cellulose (CB) were studied. The piglets were fed ad libitum for 30 d. Lower Escherichia coli (E. coli) populations in WB group and higher probiotic (Lactobacillus and Bifidobacterium) populations in groups fed diets containing arabinoxylan (WB, AX and CB) were observed and compared with CON group. Compared with CON group, the gene expressions of cystic fibrosis transmembrane conductance regulator (CFTR), calcium-activated chloride channel regulator 1 (CLCA1) and voltage-gated chloride channel 2 (CIC2) were suppressed in the WB group. And wheat bran down-regulated gene expression of pro-inflammation (TNF-alpha, IL-1 beta, IL-6) and TLRs/MyD88/NF-kappa B pathway compared with CON group. In conclusion, wheat bran and its main polysaccharides could change intestinal microflora and down-regulate the gene expression of intestinal barrier function relevant proteins in the distal small intestinal mucosa.},
  author       = {Chen, Hong and Chen, Daiwen and Qin, Wen and Liu, Yuntao and Che, Lianqiang and Huang, Zhiqing and Luo, Yuheng and Zhang, Qing and Lin, Derong and Liu, Yaowen and Han, Guoquan and De Smet, Stefaan and Michiels, Joris},
  issn         = {0963-7486},
  journal      = {INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION},
  language     = {eng},
  number       = {1},
  pages        = {65--72},
  title        = {Wheat bran components modulate intestinal bacteria and gene expression of barrier function relevant proteins in a piglet model},
  url          = {http://dx.doi.org/10.1080/09637486.2016.1212817},
  volume       = {68},
  year         = {2017},
}

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