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The impact of species and cell type on the nanosafety profile of iron oxide nanoparticles in neural cells

Freya Joris, Daniel Valdepérez, Beatriz Pelaz, Stefaan J Soenen, Bella B Manshian, Wolfgang J Parak, Stefaan De Smedt UGent and Koen Raemdonck UGent (2016) JOURNAL OF NANOBIOTECHNOLOGY. 14.
abstract
Background: While nanotechnology is advancing rapidly, nanosafety tends to lag behind since general mechanistic insights into cell-nanoparticle (NP) interactions remain rare. To tackle this issue, standardization of nanosafety assessment is imperative. In this regard, we believe that the cell type selection should not be overlooked since the applicability of cell lines could be questioned given their altered phenotype. Hence, we evaluated the impact of the cell type on in vitro nanosafety evaluations in a human and murine neuroblastoma cell line, neural progenitor cell line and in neural stem cells. Acute toxicity was evaluated for gold, silver and iron oxide (IO) NPs, and the latter were additionally subjected to a multiparametric analysis to assess sublethal effects. Results: The stem cells and murine neuroblastoma cell line respectively showed most and least acute cytotoxicity. Using high content imaging, we observed cell type-and species-specific responses to the IONPs on the level of reactive oxygen species production, calcium homeostasis, mitochondrial integrity and cell morphology, indicating that cellular homeostasis is impaired in distinct ways. Conclusions: Our data reveal cell type-specific toxicity profiles and demonstrate that a single cell line or toxicity end point will not provide sufficient information on in vitro nanosafety. We propose to identify a set of standard cell lines for screening purposes and to select cell types for detailed nanosafety studies based on the intended application and/or expected exposure.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
Multiparametric analysis, IN-VITRO CYTOTOXICITY, OXIDATIVE STRESS, SILVER NANOPARTICLES, Stem cells, Iron oxide nanoparticles, Inorganic nanoparticles, Nanosafety, High content imaging, MITOCHONDRIAL ACTIVITY, GENE-EXPRESSION, DOSE-RESPONSE, LINES, STEM, TOXICITY, CALCIUM
journal title
JOURNAL OF NANOBIOTECHNOLOGY
J. Nanobiotechnol.
volume
14
article number
69
pages
13 pages
Web of Science type
Article
Web of Science id
000383754100001
JCR category
BIOTECHNOLOGY & APPLIED MICROBIOLOGY
JCR impact factor
4.946 (2016)
JCR rank
19/158 (2016)
JCR quartile
1 (2016)
ISSN
1477-3155
DOI
10.1186/s12951-016-0220-y
language
English
UGent publication?
yes
classification
A1
additional info
the last two authors contributed equally to this work
copyright statement
I have retained and own the full copyright for this publication
id
8133095
handle
http://hdl.handle.net/1854/LU-8133095
date created
2016-11-04 14:17:26
date last changed
2018-06-22 12:40:23
@article{8133095,
  abstract     = {Background: While nanotechnology is advancing rapidly, nanosafety tends to lag behind since general mechanistic insights into cell-nanoparticle (NP) interactions remain rare. To tackle this issue, standardization of nanosafety assessment is imperative. In this regard, we believe that the cell type selection should not be overlooked since the applicability of cell lines could be questioned given their altered phenotype. Hence, we evaluated the impact of the cell type on in vitro nanosafety evaluations in a human and murine neuroblastoma cell line, neural progenitor cell line and in neural stem cells. Acute toxicity was evaluated for gold, silver and iron oxide (IO) NPs, and the latter were additionally subjected to a multiparametric analysis to assess sublethal effects. 
Results: The stem cells and murine neuroblastoma cell line respectively showed most and least acute cytotoxicity. Using high content imaging, we observed cell type-and species-specific responses to the IONPs on the level of reactive oxygen species production, calcium homeostasis, mitochondrial integrity and cell morphology, indicating that cellular homeostasis is impaired in distinct ways. 
Conclusions: Our data reveal cell type-specific toxicity profiles and demonstrate that a single cell line or toxicity end point will not provide sufficient information on in vitro nanosafety. We propose to identify a set of standard cell lines for screening purposes and to select cell types for detailed nanosafety studies based on the intended application and/or expected exposure.},
  articleno    = {69},
  author       = {Joris, Freya and Valdep{\'e}rez, Daniel and Pelaz, Beatriz and Soenen, Stefaan J and Manshian, Bella B and Parak, Wolfgang J and De Smedt, Stefaan and Raemdonck, Koen},
  issn         = {1477-3155},
  journal      = {JOURNAL OF NANOBIOTECHNOLOGY},
  keyword      = {Multiparametric analysis,IN-VITRO CYTOTOXICITY,OXIDATIVE STRESS,SILVER NANOPARTICLES,Stem cells,Iron oxide nanoparticles,Inorganic nanoparticles,Nanosafety,High content imaging,MITOCHONDRIAL ACTIVITY,GENE-EXPRESSION,DOSE-RESPONSE,LINES,STEM,TOXICITY,CALCIUM},
  language     = {eng},
  pages        = {13},
  title        = {The impact of species and cell type on the nanosafety profile of iron oxide nanoparticles in neural cells},
  url          = {http://dx.doi.org/10.1186/s12951-016-0220-y},
  volume       = {14},
  year         = {2016},
}

Chicago
Joris, Freya, Daniel Valdepérez, Beatriz Pelaz, Stefaan J Soenen, Bella B Manshian, Wolfgang J Parak, Stefaan De Smedt, and Koen Raemdonck. 2016. “The Impact of Species and Cell Type on the Nanosafety Profile of Iron Oxide Nanoparticles in Neural Cells.” Journal of Nanobiotechnology 14.
APA
Joris, F., Valdepérez, D., Pelaz, B., Soenen, S. J., Manshian, B. B., Parak, W. J., De Smedt, S., et al. (2016). The impact of species and cell type on the nanosafety profile of iron oxide nanoparticles in neural cells. JOURNAL OF NANOBIOTECHNOLOGY, 14.
Vancouver
1.
Joris F, Valdepérez D, Pelaz B, Soenen SJ, Manshian BB, Parak WJ, et al. The impact of species and cell type on the nanosafety profile of iron oxide nanoparticles in neural cells. JOURNAL OF NANOBIOTECHNOLOGY. 2016;14.
MLA
Joris, Freya, Daniel Valdepérez, Beatriz Pelaz, et al. “The Impact of Species and Cell Type on the Nanosafety Profile of Iron Oxide Nanoparticles in Neural Cells.” JOURNAL OF NANOBIOTECHNOLOGY 14 (2016): n. pag. Print.