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p120 Catenin-mediated stabilization of E-cadherin is essential for primitive endoderm specification

Tim Pieters (UGent) , Steven Goossens (UGent) , Lieven Haenebalcke (UGent) , Vanessa Andries (UGent) , Agata Stryjewska, Riet De Rycke (UGent) , Kelly Lemeire (UGent) , Tino Hochepied (UGent) , Danny Huylebroeck, Geert Berx (UGent) , et al.
(2016) PLOS GENETICS. 12(8).
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Abstract
E-cadherin-mediated cell-cell adhesion is critical for naive pluripotency of cultured mouse embryonic stem cells (mESCs). E-cadherin-depleted mESC fail to downregulate their pluripotency program and are unable to initiate lineage commitment. To further explore the roles of cell adhesion molecules during mESC differentiation, we focused on p120 catenin (p120ctn). Although one key function of p120ctn is to stabilize and regulate cadherin-mediated cell-cell adhesion, it has many additional functions, including regulation of transcription and Rho GTPase activity. Here, we investigated the role of mouse p120ctn in early embryogenesis, mESC pluripotency and early fate determination. In contrast to the E-cadherin-null phenotype, p120ctn-null mESCs remained pluripotent, but their in vitro differentiation was incomplete. In particular, they failed to form cystic embryoid bodies and showed defects in primitive endoderm formation. To pinpoint the underlying mechanism, we undertook a structure-function approach. Rescue of p120ctn-null mESCs with different p120ctn wild-type and mutant expression constructs revealed that the long N-terminal domain of p120ctn and its regulatory domain for RhoA were dispensable, whereas its armadillo domain and interaction with E-cadherin were crucial for primitive endoderm formation. We conclude that p120ctn is not only an adaptor and regulator of E-cadherin, but is also indispensable for proper lineage commitment.
Keywords
ADHERENS JUNCTIONS, NUCLEAR-LOCALIZATION, SELF-RENEWAL, TRANSCRIPTION FACTOR, IN-VITRO, MOUSE, DIFFERENTIATION, ADHESION, RHO-FAMILY GTPASES, EMBRYONIC STEM-CELLS

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Chicago
Pieters, Tim, Steven Goossens, Lieven Haenebalcke, Vanessa Andries, Agata Stryjewska, Riet De Rycke, Kelly Lemeire, et al. 2016. “P120 Catenin-mediated Stabilization of E-cadherin Is Essential for Primitive Endoderm Specification.” Plos Genetics 12 (8).
APA
Pieters, Tim, Goossens, S., Haenebalcke, L., Andries, V., Stryjewska, A., De Rycke, R., Lemeire, K., et al. (2016). p120 Catenin-mediated stabilization of E-cadherin is essential for primitive endoderm specification. PLOS GENETICS, 12(8).
Vancouver
1.
Pieters T, Goossens S, Haenebalcke L, Andries V, Stryjewska A, De Rycke R, et al. p120 Catenin-mediated stabilization of E-cadherin is essential for primitive endoderm specification. PLOS GENETICS. 2016;12(8).
MLA
Pieters, Tim, Steven Goossens, Lieven Haenebalcke, et al. “P120 Catenin-mediated Stabilization of E-cadherin Is Essential for Primitive Endoderm Specification.” PLOS GENETICS 12.8 (2016): n. pag. Print.
@article{8132550,
  abstract     = {E-cadherin-mediated cell-cell adhesion is critical for naive pluripotency of cultured mouse embryonic stem cells (mESCs). E-cadherin-depleted mESC fail to downregulate their pluripotency program and are unable to initiate lineage commitment. To further explore the roles of cell adhesion molecules during mESC differentiation, we focused on p120 catenin (p120ctn). Although one key function of p120ctn is to stabilize and regulate cadherin-mediated cell-cell adhesion, it has many additional functions, including regulation of transcription and Rho GTPase activity. Here, we investigated the role of mouse p120ctn in early embryogenesis, mESC pluripotency and early fate determination. In contrast to the E-cadherin-null phenotype, p120ctn-null mESCs remained pluripotent, but their in vitro differentiation was incomplete. In particular, they failed to form cystic embryoid bodies and showed defects in primitive endoderm formation. To pinpoint the underlying mechanism, we undertook a structure-function approach. Rescue of p120ctn-null mESCs with different p120ctn wild-type and mutant expression constructs revealed that the long N-terminal domain of p120ctn and its regulatory domain for RhoA were dispensable, whereas its armadillo domain and interaction with E-cadherin were crucial for primitive endoderm formation. We conclude that p120ctn is not only an adaptor and regulator of E-cadherin, but is also indispensable for proper lineage commitment.},
  articleno    = {e1006243},
  author       = {Pieters, Tim and Goossens, Steven and Haenebalcke, Lieven and Andries, Vanessa and Stryjewska, Agata and De Rycke, Riet and Lemeire, Kelly and Hochepied, Tino and Huylebroeck, Danny and Berx, Geert and Stemmler, Marc P and Wirth, Dagmar and Haigh, Jody and van Hengel, Jolanda and Van Roy, Frans},
  issn         = {1553-7404},
  journal      = {PLOS GENETICS},
  language     = {eng},
  number       = {8},
  pages        = {28},
  title        = {p120 Catenin-mediated stabilization of E-cadherin is essential for primitive endoderm specification},
  url          = {http://dx.doi.org/10.1371/journal.pgen.1006243},
  volume       = {12},
  year         = {2016},
}

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