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Equine herpesvirus-1 myeloencephalopathy, an emerging threat of working equids in Ethiopia

Haileleul Negussie-Dubale UGent, Daniel Gizaw, Tesfaye Tessema and Hans Nauwynck UGent (2017) TRANSBOUNDARY AND EMERGING DISEASES. 64(2). p.389-397
abstract
Although equine herpesvirus myeloencephalopathy (EHM) is a sporadic and relatively uncommon manifestation of equine herpesvirus-1 (EHV-1), it has the potential for causing devastating outbreaks in horses. Up till now, there were no reported EHM outbreaks in donkeys and mules. This study describes the isolation and molecular characterization of EHV-1 from clinically EHM-affected horses (n = 6), mules (n = 3) and donkeys (n = 82) in Ethiopia during outbreaks from May 2011 to December 2013. The incidence of EHM cases was higher from April to mid-June. EHM in donkeys was more severe and death without clinical signs of paralysis, and recumbency was frequently observed. The main age of affected equines ranged from 7 to 10 years (n = 51; 56.0%), and females (n = 58; 63.7%) were more affected than males. The incidence of neuropathogenic (D752) and non-neuropathogenic (N752) variants of EHV-1 from EHM-affected equines in Ethiopia was assessed by sequencing the DNA polymerase gene (ORF30) of the EHV-1 isolates. The results indicated that from the total of 91 clinically affected equines, 90 (98.9%) of them had an ORF30 D752 genotype. An ORF30 N752 variant was only found in one donkey. Analysis of ORF68 as grouping marker for geographical differences showed that the Ethiopian EHV-1 isolates belong to geographical group 4. Due to the fatal nature of EHV-1 in donkeys, it would be interesting to examine the pathogenesis of EHM in this species. At present, there is no vaccine available in Ethiopia, and therefore, outbreaks of EHV-1 should be controlled by proper management adaptations. In addition, it is important to test the efficacy of the commercial vaccines not only in horses, but also in donkeys and mules.
Please use this url to cite or link to this publication:
author
organization
year
type
journalArticle (original)
publication status
published
subject
keyword
equine herpesvirus-1, Ethiopia, equines, equine herpesvirus myeloencephalopathy, outbreaks, POLYMERASE CHAIN-REACTION, VIROLOGICAL CHARACTERISTICS, REPLICATION KINETICS, NEUROLOGICAL DISEASE, DNA-POLYMERASE, OUTBREAK, TYPE-1, STRAINS, HORSES, EHV-1
journal title
TRANSBOUNDARY AND EMERGING DISEASES
Transbound. Emerg. Dis.
volume
64
issue
2
pages
389 - 397
Web of Science type
Article
Web of Science id
000396836000008
ISSN
1865-1674
DOI
10.1111/tbed.12377
language
English
UGent publication?
yes
classification
A1
copyright statement
I have transferred the copyright for this publication to the publisher
id
8132348
handle
http://hdl.handle.net/1854/LU-8132348
date created
2016-11-03 13:34:48
date last changed
2017-07-24 09:07:41
@article{8132348,
  abstract     = {Although equine herpesvirus myeloencephalopathy (EHM) is a sporadic and relatively uncommon manifestation of equine herpesvirus-1 (EHV-1), it has the potential for causing devastating outbreaks in horses. Up till now, there were no reported EHM outbreaks in donkeys and mules. This study describes the isolation and molecular characterization of EHV-1 from clinically EHM-affected horses (n = 6), mules (n = 3) and donkeys (n = 82) in Ethiopia during outbreaks from May 2011 to December 2013. The incidence of EHM cases was higher from April to mid-June. EHM in donkeys was more severe and death without clinical signs of paralysis, and recumbency was frequently observed. The main age of affected equines ranged from 7 to 10 years (n = 51; 56.0\%), and females (n = 58; 63.7\%) were more affected than males. The incidence of neuropathogenic (D752) and non-neuropathogenic (N752) variants of EHV-1 from EHM-affected equines in Ethiopia was assessed by sequencing the DNA polymerase gene (ORF30) of the EHV-1 isolates. The results indicated that from the total of 91 clinically affected equines, 90 (98.9\%) of them had an ORF30 D752 genotype. An ORF30 N752 variant was only found in one donkey. Analysis of ORF68 as grouping marker for geographical differences showed that the Ethiopian EHV-1 isolates belong to geographical group 4. Due to the fatal nature of EHV-1 in donkeys, it would be interesting to examine the pathogenesis of EHM in this species. At present, there is no vaccine available in Ethiopia, and therefore, outbreaks of EHV-1 should be controlled by proper management adaptations. In addition, it is important to test the efficacy of the commercial vaccines not only in horses, but also in donkeys and mules.},
  author       = {Negussie-Dubale, Haileleul and Gizaw, Daniel and Tessema, Tesfaye and Nauwynck, Hans},
  issn         = {1865-1674},
  journal      = {TRANSBOUNDARY AND EMERGING DISEASES},
  keyword      = {equine herpesvirus-1,Ethiopia,equines,equine herpesvirus myeloencephalopathy,outbreaks,POLYMERASE CHAIN-REACTION,VIROLOGICAL CHARACTERISTICS,REPLICATION KINETICS,NEUROLOGICAL DISEASE,DNA-POLYMERASE,OUTBREAK,TYPE-1,STRAINS,HORSES,EHV-1},
  language     = {eng},
  number       = {2},
  pages        = {389--397},
  title        = {Equine herpesvirus-1 myeloencephalopathy, an emerging threat of working equids in Ethiopia},
  url          = {http://dx.doi.org/10.1111/tbed.12377},
  volume       = {64},
  year         = {2017},
}

Chicago
Negussie-Dubale, Haileleul, Daniel Gizaw, Tesfaye Tessema, and Hans Nauwynck. 2017. “Equine Herpesvirus-1 Myeloencephalopathy, an Emerging Threat of Working Equids in Ethiopia.” Transboundary and Emerging Diseases 64 (2): 389–397.
APA
Negussie-Dubale, H., Gizaw, D., Tessema, T., & Nauwynck, H. (2017). Equine herpesvirus-1 myeloencephalopathy, an emerging threat of working equids in Ethiopia. TRANSBOUNDARY AND EMERGING DISEASES, 64(2), 389–397.
Vancouver
1.
Negussie-Dubale H, Gizaw D, Tessema T, Nauwynck H. Equine herpesvirus-1 myeloencephalopathy, an emerging threat of working equids in Ethiopia. TRANSBOUNDARY AND EMERGING DISEASES. 2017;64(2):389–97.
MLA
Negussie-Dubale, Haileleul, Daniel Gizaw, Tesfaye Tessema, et al. “Equine Herpesvirus-1 Myeloencephalopathy, an Emerging Threat of Working Equids in Ethiopia.” TRANSBOUNDARY AND EMERGING DISEASES 64.2 (2017): 389–397. Print.