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Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity

(2012) PLOS ONE. 7(10).
Author
Organization
Abstract
Analysis of the tsetse fly salivary gland EST database revealed the presence of a highly enriched cluster of putative endonuclease genes, including tsal1 and tsal2. Tsal proteins are the major components of tsetse fly (G. morsitans morsitans) saliva where they are present as monomers as well as high molecular weight complexes with other saliva proteins. We demonstrate that the recombinant tsetse salivary gland proteins 1&2 (Tsal1&2) display DNA/RNA non-specific, high affinity nucleic acid binding with K-D values in the low nanomolar range and a non-exclusive preference for duplex. These Tsal proteins exert only a residual nuclease activity with a preference for dsDNA in a broad pH range. Knockdown of Tsal expression by in vivo RNA interference in the tsetse fly revealed a partially impaired blood digestion phenotype as evidenced by higher gut nucleic acid, hematin and protein contents.
Keywords
GLOSSINA-MORSITANS-MORSITANS, SERRATIA-MARCESCENS ENDONUCLEASE, NEUTROPHIL EXTRACELLULAR TRAPS, DUPLEX-SPECIFIC NUCLEASE, FLY SALIVA, PLATELET-AGGREGATION, ESCHERICHIA-COLI, IDENTIFICATION, MECHANISM, HEPATOPANCREAS

Citation

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Chicago
Caljon, Guy, Karin De Ridder, Benoit Stijlemans, Marc Coosemans, Stefan Magez, Patrick De Baetselier, and Jan Van den Abbeele. 2012. “Tsetse Salivary Gland Proteins 1 and 2 Are High Affinity Nucleic Acid Binding Proteins with Residual Nuclease Activity.” Plos One 7 (10).
APA
Caljon, Guy, De Ridder, K., Stijlemans, B., Coosemans, M., Magez, S., De Baetselier, P., & Van den Abbeele, J. (2012). Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity. PLOS ONE, 7(10).
Vancouver
1.
Caljon G, De Ridder K, Stijlemans B, Coosemans M, Magez S, De Baetselier P, et al. Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity. PLOS ONE. 2012;7(10).
MLA
Caljon, Guy et al. “Tsetse Salivary Gland Proteins 1 and 2 Are High Affinity Nucleic Acid Binding Proteins with Residual Nuclease Activity.” PLOS ONE 7.10 (2012): n. pag. Print.
@article{8131714,
  abstract     = {Analysis of the tsetse fly salivary gland EST database revealed the presence of a highly enriched cluster of putative endonuclease genes, including tsal1 and tsal2. Tsal proteins are the major components of tsetse fly (G. morsitans morsitans) saliva where they are present as monomers as well as high molecular weight complexes with other saliva proteins. We demonstrate that the recombinant tsetse salivary gland proteins 1\&2 (Tsal1\&2) display DNA/RNA non-specific, high affinity nucleic acid binding with K-D values in the low nanomolar range and a non-exclusive preference for duplex. These Tsal proteins exert only a residual nuclease activity with a preference for dsDNA in a broad pH range. Knockdown of Tsal expression by in vivo RNA interference in the tsetse fly revealed a partially impaired blood digestion phenotype as evidenced by higher gut nucleic acid, hematin and protein contents.},
  articleno    = {e47233},
  author       = {Caljon, Guy and De Ridder, Karin and Stijlemans, Benoit and Coosemans, Marc and Magez, Stefan and De Baetselier, Patrick and Van den Abbeele, Jan},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {10},
  pages        = {14},
  title        = {Tsetse salivary gland proteins 1 and 2 are high affinity nucleic acid binding proteins with residual nuclease activity},
  url          = {http://dx.doi.org/10.1371/journal.pone.0047233},
  volume       = {7},
  year         = {2012},
}

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