Advanced search

Comparative evaluation of the nested ITS PCR against the 18S PCR-RFLP in a survey of bovine trypanosomiasis in Kwale County, Kenya

Author
Organization
Abstract
We compared the nested internal transcribed spacer (ITS) PCR and the 18S PCR-RFLP (restriction-fragment length polymorphism) pan-trypanosome assays in a cross-sectional survey of bovine trypanosomiasis in 358 cattle in Kwale County, Kenya. The prevalence of trypanosomiasis as determined by the nested ITS PCR was 19.6% (70/358) and by 18S PCR-RFLP was 16.8% (60/358). Of the pathogenic trypanosomes detected, the prevalence of Trypanosoma congolense and Trypanosoma vivax was greater than that of Trypanosoma simiae. The nested ITS PCR detected 83 parasite events, whereas the 18S PCR-RFLP detected 64; however, overall frequencies of infections and the parasite events detected did not differ between the assays ((2) = 0.8, df = 1, p > 0.05 and (2) = 2.5, df = 1, p > 0.05, respectively). The kappa statistic (0.8) showed good agreement between the tests. The nested ITS PCR and the 18S PCR-RFLP had comparable sensitivity, although the nested ITS PCR was better at detecting mixed infections ((2) = 5.4, df = 1, p < 0.05).
Keywords
Bovine trypanosomiasis, Kenya, Kwale County, nested ITS PCR, 18S PCR-RFLP, SSU-RDNA AMPLIFICATION, CATTLE, CONGOLENSE, PATHOGENICITY, DIAGNOSIS, LIVESTOCK, BLOOD

Citation

Please use this url to cite or link to this publication:

Chicago
Odongo, Steven, Vincent Delespaux, Maina Ngotho, Serkalem Mindaye Bekkele, and Stefan Magez. 2016. “Comparative Evaluation of the Nested ITS PCR Against the 18S PCR-RFLP in a Survey of Bovine Trypanosomiasis in Kwale County, Kenya.” Journal of Veterinary Diagnostic Investigation 28 (5): 589–594.
APA
Odongo, Steven, Delespaux, V., Ngotho, M., Bekkele, S. M., & Magez, S. (2016). Comparative evaluation of the nested ITS PCR against the 18S PCR-RFLP in a survey of bovine trypanosomiasis in Kwale County, Kenya. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, 28(5), 589–594.
Vancouver
1.
Odongo S, Delespaux V, Ngotho M, Bekkele SM, Magez S. Comparative evaluation of the nested ITS PCR against the 18S PCR-RFLP in a survey of bovine trypanosomiasis in Kwale County, Kenya. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. 2016;28(5):589–94.
MLA
Odongo, Steven, Vincent Delespaux, Maina Ngotho, et al. “Comparative Evaluation of the Nested ITS PCR Against the 18S PCR-RFLP in a Survey of Bovine Trypanosomiasis in Kwale County, Kenya.” JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 28.5 (2016): 589–594. Print.
@article{8131499,
  abstract     = {We compared the nested internal transcribed spacer (ITS) PCR and the 18S PCR-RFLP (restriction-fragment length polymorphism) pan-trypanosome assays in a cross-sectional survey of bovine trypanosomiasis in 358 cattle in Kwale County, Kenya. The prevalence of trypanosomiasis as determined by the nested ITS PCR was 19.6\% (70/358) and by 18S PCR-RFLP was 16.8\% (60/358). Of the pathogenic trypanosomes detected, the prevalence of Trypanosoma congolense and Trypanosoma vivax was greater than that of Trypanosoma simiae. The nested ITS PCR detected 83 parasite events, whereas the 18S PCR-RFLP detected 64; however, overall frequencies of infections and the parasite events detected did not differ between the assays ((2) = 0.8, df = 1, p {\textrangle} 0.05 and (2) = 2.5, df = 1, p {\textrangle} 0.05, respectively). The kappa statistic (0.8) showed good agreement between the tests. The nested ITS PCR and the 18S PCR-RFLP had comparable sensitivity, although the nested ITS PCR was better at detecting mixed infections ((2) = 5.4, df = 1, p {\textlangle} 0.05).},
  author       = {Odongo, Steven and Delespaux, Vincent and Ngotho, Maina and Bekkele, Serkalem Mindaye and Magez, Stefan},
  issn         = {1040-6387},
  journal      = {JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION},
  keyword      = {Bovine trypanosomiasis,Kenya,Kwale County,nested ITS PCR,18S PCR-RFLP,SSU-RDNA AMPLIFICATION,CATTLE,CONGOLENSE,PATHOGENICITY,DIAGNOSIS,LIVESTOCK,BLOOD},
  language     = {eng},
  number       = {5},
  pages        = {589--594},
  title        = {Comparative evaluation of the nested ITS PCR against the 18S PCR-RFLP in a survey of bovine trypanosomiasis in Kwale County, Kenya},
  url          = {http://dx.doi.org/10.1177/1040638716659100},
  volume       = {28},
  year         = {2016},
}

Altmetric
View in Altmetric
Web of Science
Times cited: