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Transfer to the milk of rumen bypass CLA emulsions created by potato tuber peel polyphenol oxidase

Frederik Gadeyne, Nympha De Neve UGent, Bruno Vlaeminck UGent, Paul Van Der Meeren UGent and Veerle Fievez UGent (2016) Euro Fed Lipid congress, 14th, Abstracts. p.165-165
abstract
Rumen bypass technologies aim at protecting nutrients, for example polyunsaturated fatty acids against degradation, in this case biohydrogenation, to ensure an unaffected passage through the rumen and enhanced intestinal absorption. Recently, a new encapsulation technology has been proposed based on the properties of the naturally occurring enzyme polyphenol oxidase (PPO). However, up till now no in vivo approval of the PPO-based bypass technology nor evidence of post-ruminal absorption is available. Proteins and PPO in this study were extracted from potato (Solanum tuberosum L.) tuber peels and used to emulsify triacylglycerols of a mixture of conjugated linoleic acid (CLA) isomers (Tonalin TG80, BASF-AG, Ludwigshafen, Germany) in water (20 mg oil per ml extract) before encapsulation was created by the cross-linking of interfacial proteins upon addition of the diphenol 4-methylcatechol (20 mM). This rumen bypass CLA emulsion or a commercially available protected CLA product (Lutrell Combi, BASF-AG, Ludwigshafen, Germany) as positive control was used to dose 7 g trans-10, cis-12 C18:2 per day to eight dairy cows during five consecutive days in a sequential set-up. Milk fat content and fatty acid composition was monitored to assess the transfer to milk of dietary CLA. Evidence for the protection in vivo against ruminal degradation, post-ruminal uptake and transfer to the milk was given as increased proportions of trans-10, cis-12 C18:2 were found in milk fat of cows compared with the periods before and after supplementation of both additives. Extra proof for the transfer to the milk was given because both supplements resulted in similar reductions in milk fat content, since trans-10, cis-12 C18:2 is known as an inhibitor of milk fat synthesis if sufficient amounts of this CLA reach the mammary gland. However, somewhat lower transfer efficiencies of trans-10, cis-12 C18:2 from the diet to the milk in the case of the rumen bypass CLA emulsions compared with the positive control and the occurrence of non-negligible levels of CLA in faecal material indicates there is still scope for further improvement of the new technology. In conclusion, the PPO based lipid protection technology could provide a worthy alternative for current rumen bypass products as the encapsulated CLA was effectively transferred to the milk.
Please use this url to cite or link to this publication:
author
organization
year
type
conference (meetingAbstract)
publication status
published
subject
in
Euro Fed Lipid congress, 14th, Abstracts
pages
165 - 165
conference name
14th Euro Fed Lipid congress: Fats, oils and lipids : innovative approaches towards a sustainable future
conference location
Ghent, Belgium
conference start
2016-09-18
conference end
2016-09-21
language
English
UGent publication?
yes
classification
C3
id
8130663
handle
http://hdl.handle.net/1854/LU-8130663
date created
2016-10-28 10:57:33
date last changed
2017-06-21 09:42:25
@inproceedings{8130663,
  abstract     = {Rumen bypass technologies aim at protecting nutrients, for example polyunsaturated fatty acids against degradation, in this case biohydrogenation, to ensure an unaffected passage through the rumen and enhanced intestinal absorption. Recently, a new encapsulation technology has been proposed based on the properties of the naturally occurring enzyme polyphenol oxidase (PPO). However, up till now no in vivo approval of the PPO-based bypass technology nor evidence of post-ruminal absorption is available. Proteins and PPO in this study were extracted from potato (Solanum tuberosum L.) tuber peels and used to emulsify triacylglycerols of a mixture of conjugated linoleic acid (CLA) isomers (Tonalin TG80, BASF-AG, Ludwigshafen, Germany) in water (20 mg oil per ml extract) before encapsulation was created by the cross-linking of interfacial proteins upon addition of the diphenol 4-methylcatechol (20 mM). This rumen bypass CLA emulsion or a commercially available protected CLA product (Lutrell Combi, BASF-AG, Ludwigshafen, Germany) as positive control was used to dose 7 g trans-10, cis-12 C18:2 per day to eight dairy cows during five consecutive days in a sequential set-up. Milk fat content and fatty acid composition was monitored to assess the transfer to milk of dietary CLA. Evidence for the protection in vivo against ruminal degradation, post-ruminal uptake and transfer to the milk was given as increased proportions of trans-10, cis-12 C18:2 were found in milk fat of cows compared with the periods before and after supplementation of both additives. Extra proof for the transfer to the milk was given because both supplements resulted in similar reductions in milk fat content, since trans-10, cis-12 C18:2 is known as an inhibitor of milk fat synthesis if sufficient amounts of this CLA reach the mammary gland. However, somewhat lower transfer efficiencies of trans-10, cis-12 C18:2 from the diet to the milk in the case of the rumen bypass CLA emulsions compared with the positive control and the occurrence of non-negligible levels of CLA in faecal material indicates there is still scope for further improvement of the new technology. In conclusion, the PPO based lipid protection technology could provide a worthy alternative for current rumen bypass products as the encapsulated CLA was effectively transferred to the milk.},
  author       = {Gadeyne, Frederik and De Neve, Nympha and Vlaeminck, Bruno and Van Der Meeren, Paul and Fievez, Veerle},
  booktitle    = {Euro Fed Lipid congress, 14th, Abstracts},
  language     = {eng},
  location     = {Ghent, Belgium},
  pages        = {165--165},
  title        = {Transfer to the milk of rumen bypass CLA emulsions created by potato tuber peel polyphenol oxidase},
  year         = {2016},
}

Chicago
Gadeyne, Frederik, Nympha De Neve, Bruno Vlaeminck, Paul Van Der Meeren, and Veerle Fievez. 2016. “Transfer to the Milk of Rumen Bypass CLA Emulsions Created by Potato Tuber Peel Polyphenol Oxidase.” In Euro Fed Lipid Congress, 14th, Abstracts, 165–165.
APA
Gadeyne, F., De Neve, N., Vlaeminck, B., Van Der Meeren, P., & Fievez, V. (2016). Transfer to the milk of rumen bypass CLA emulsions created by potato tuber peel polyphenol oxidase. Euro Fed Lipid congress, 14th, Abstracts (pp. 165–165). Presented at the 14th Euro Fed Lipid congress: Fats, oils and lipids : innovative approaches towards a sustainable future.
Vancouver
1.
Gadeyne F, De Neve N, Vlaeminck B, Van Der Meeren P, Fievez V. Transfer to the milk of rumen bypass CLA emulsions created by potato tuber peel polyphenol oxidase. Euro Fed Lipid congress, 14th, Abstracts. 2016. p. 165–165.
MLA
Gadeyne, Frederik, Nympha De Neve, Bruno Vlaeminck, et al. “Transfer to the Milk of Rumen Bypass CLA Emulsions Created by Potato Tuber Peel Polyphenol Oxidase.” Euro Fed Lipid Congress, 14th, Abstracts. 2016. 165–165. Print.